Sipa1l1 (BC058681) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4931426N11Rik; AW213287; mKIAA0440; Spar
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212077 representing BC058681 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGTTTGAAGCGGTCGCAGACCGAAAGACCTGTCACCGCTGACAGAGCCTCTGTTGTCAGCACAG ATGGCGCCCCCAAAGTCCACACCGATGACTTCTACATGCGTCGCTTCCGCTCTCAGAATGGCAGCCTAGG ATCATCAGTCATGGCTGCAGTGGGGCCCCCTCGAAGTGAAGGCCCTCACCATATCACCTCAACCCCCGGG GTCCCCAAGATGGGGGTTAGGGCAAGAATAGCAGATTGGCCTCCGAGAAAGGAAAATGTAAAAGAATCCA GCCGTTCAAGCCAGGAAATAGAAACCTCAAGTTGCCTTGAGAGCCTGTCCTCCAAAGGCAGTCCTGTGAG TCAGGGGAGTTCTGTTAGCCTCAATTCCAATGACTCAGCCATGCTGAAGAGCATACAGAACACCCTGAAG AACAAGACAGGGCCAGCGGAGAGCATGGACTCCAGATTCCTCATGCCTGAAGCCTACCCCAGTTCCCCCA GGAAAGCCCTTCGCAGAATTCGGCAGCGCAGCAACAGTGATATCACCATAAGTGAGCTTGATGTGGATAG CTTCGATGAATGTATCTCCCCAACCTACAAGTCGGGGCCATCATTGCACAGGGAATATGGCAGCACATCT TCAATCGACAAGCAGGGAACATCCGGAGACAGCTTCTTCGATTTGTTAAAGGGCTATAAAGATGACAGAT CTGACCGAGGTCCAACTCCAACCAAACTCAGTGACTTCCTCATCACTGGTGGGGGCAAGGGTTCTGGTTT CTCCTTGGATGTGATCGATGGCCCCATCTCACAGAGAGAGAACCTCAGGCTTTTCAAGGAAAGGGAAAAA CCACTCAAGCGACGCTCTAAGTCTGAGACTGGAGACTCGTCCATTTTTCGTAAATTGCGCAATGCCAAAG GTGAAGAACTCGGGAAATCATCAGACCTTGAAGACAACAGATCAGAAGATTCTGTGAGGCCCTGGACATG TCCAAAGTGCTTTGCCCACTATGATGTCCAGAGCATATTGTTTGACTTGAATGAAGCCATTATGAACAGA CATAATGTGATTAAGAGGAGAAACACCACAACAGGAGCTTCGGCGGCTGCGGTGGCATCCTTGGTCTCCG GACCTCTGTCTCACTCAGCCAGCTTCAGCTCTCCCATGGGCAGCACAGAGGACCTCAACTCCAAAGGAAG CCTTGGCATGGACCAGGGAGATGACAAGAGCAATGAACTCGTCATGAGCTGTCCGTATTTTCGGAATGAG ATTGGGGGAGAAGGTGAGAGGAAGATCAGCCTGTCCAAGTCGAATTCTGGCTCATTTAGTGGGTGTGAGA GCACATCCTTTGAGTCTGCCCTCAGCTCTCACTGCACCAACGCGGGCGTGGCAGTTCTCGAAGTGCCCAA GGAAAGCTTGATGCTGCATCTGGACAGGGTGAAAAGGTACACCGTGGAACACGTGGATCTTGGCGCATAC TATTACAGGAAGTTCTTCTACCAGAAGGAACACTGGAACTATTTTGGGGCTGATGAGAACCTCGGTCCAG TGGCTGTGAGCATTCGAAGGGAAAAACCAGAAGACATGAAGGAAAACGGATCTCCATACAACTACCGAAT AATATTCAGGACTAGTGAGCTCATGACGCTGAGGGGGTCTGTCCTGGAGGATGCCATTCCCTCCACGGCC AAGCACTCGACAGCCAGGGGATTGCCTCTGAAAGAGGTGCTGGAACACGTGATCCCAGAGCTCAACGTGC AGTGCCTGCGCTTGGCCTTCAACACACCCAAAGTCACAGAGCAGCTCATGAAACTGGACGAGCAAGGGCT GAACTATCAGCAGAAAGTAGGCATCATGTACTGCAAAGCAGGCCAGAGCACGGAGGAGGAGATGTACAAC AACGAGTCTGCAGGCCCAGCCTTTGAGGAGTTCCTTCAGCTGCTGGGGGAACGAGTCCGGCTAAAAGGAT TCGAGAAGTATCGTGCGCAGCTTGACACCAAAACTGACTCCACTGGAACCCACTCTCTGTACACAACCTA CAAAGACTATGAGATAATGTTCCACGTCTCCACCATGCTGCCCTACACACCTAACAACAAGCAACAGCTC CTGAGGAAGCGGCACATTGGGAATGACATTGTGACAATAGTTTTCCAAGAGCCTGGAGCACAACCATTCA GCCCGAAAAACATCCGGTCTCACTTTCAGCATGTTTTTGTCATTGTCCGGGCTCACAACCCTTGCACTGA GAGTGTCTGTTACAGTGTGGCAGTCACCAGGTCCAGAGATGTACCTTCTTTTGGACCTCCCATCCCTAAA GGGGTCACCTTCCCCAAGTCAAATGTGTTCAGGGACTTCCTTTTGGCCAAAGTGATAAATGCAGAAAATG CTGCTCATAAATCAGAGAAGTTCCGGGCCATGGCGACAAGGACCCGCCAGGAATACCTGAAAGATCTGGC AGAAAAGAATGTCACCAACACACCTATTGACCCTTCTGGCAAGTTTCCATTTATTTCTCTGGCCTCCAAG AAGAAGGAAAAGTCTAAGCCTTATCCAGGAGCTGAGCTCAGTAGCATGGGGGCCATTGTGTGGGCTGTCC GGGCCAAAGACTACAACAAGGCCATGGAGTTCGACTGCCTCCTTGGGATCTCCAGCGAGTTCATCGTCCT CATTGAGCAGGAGACAAAGAGTGTGGCTTTCAATTGCTCCTGCAGAGATGTCATAGGGTGGACTTCCAGC GACACCAGCCTCAAAATCTTCTATGAGCGGGGAGAATGTGTGTCGGTGGAGAGCTTCATTAGCGGTGAAG ATATCAAAGAAATTGTCAGAAGGCTGCAGTTTGTTTCAAAAGGTTGTGAATCTGTGGAAATGACTCTGCG AAGAAATGGGCTGGGGCAGCTTGGCTTCCATGTCAACTATGAGGGCATTGTGGCGGATGTAGAACCCTAC GGCTACGCATGGCAAGCAGGGCTGAGGCAGGGCAGCCGCCTGGTGGAGATCTGCAAGGTAGCAGTGGCCA CCCTGAGCCATGAACAGATGATCGATCTCTTGAGAACATCAGTCACAGTGAAGGTTGTCATTATCCCTCC CCATGACGACTGCACCCCACGGAGGAGTTGCTCAGAAACCTACCGCATGCCAGTGATGGAGTACCAGATG AATGAAGGCATTTCCTACGAGTTCAAGTTTCCCTTCCGGAATAATAACAAATGGCAGCGGAATGCCAGCA AGGGTGCTCATTCGCCCCAGGTTCCATCTCAGCTGCAGAGTCCCATGACCTCACGACTGAATGCTGGGAA GGGAGATGGGAAAATGCCCCCTCCAGAAAGAGCTGCCAACATCCCTCGAAGCATCTCCAGTGACGGGCGC CCACTGGAAAGGAGGCTGTCTCCTGGTTCGGACATCTATGTGACAGTCTCATCCATGGCTTTGGCGAGAT CCCAGTGCCGTAACTCTCCCAGCAACTTGTCTTCGTCCAGTGAGACTGGCTCTGGAGGTGGTACCTACAG ACAAAAATCCATGCCCGAAGGGTTTGGGGTGAGCCGAAGATCCCCAGCTTCCATCGACAGGCAGAACACC CAGTCGGATATAAGTGGCAGTGGAAAATCCACTCCCAGCTGGCAGAGAAGTGAGGACAGCCTTGCCGACC AGATGGAGCCGACGTGCCATCTCCCAGCAGTATCGAAGGTACTGCCTGCTTTCCGAGAGAGCCCCAGTGG GAGATTGATGCGGCAGGATCCAGTGGTTCACTTGTCTCCAAACAAACAAGGCCATTCTGACAGCCACTAC TCCAGCCACTCCAGCAGCAACACGCTCTCCAGCAACGCCTCGAGTGCACACAGTGACGAGAAGTGGTACG ATGGGGACCGCACGGAGTCCGACCTCAACAGCTACAACTACCTACAGGGCACGTCTGCCGACAGCGGGAT TGACACCGCCTCCTACGGCCCCAGCCATGGCAGCACGGCCTCCCTGGGGGCCTCCACATCCTCACCTCGT TCAGGGCCAGGCAAAGAAAAGGTGGCTCCCCTGTGGCACAGCTCCAGTGAAGTGCTCTCCCTGGCAGATC GGACCTTAGAGACTGAGGGCCACGGCATGGACAGGAAAGCAGAGTCCTCCCTGAGCCTGGACATCCACAG CAAGAGCCAGGGCGGGTCAAGCCCGCTGAGCAGGGAGAACAGCACCTTCAGCATAAATGATGCTGCGTCC CACACCAGTACCATGAGCTCCCGACACTCTGCCAGCCCAGTGGTATTCTCCAGTGCCAGAAGTTCCCCCA AAGAGGAGCTTCACCCCACCGCATCCTCCCAGCTCGCACCGTCCTTTTCCTCTTCTTCCTCATCCTCCTC TGGACCTAGGACTTTCTACCCTCGCCAGGGCGCCACTAGCAAATATCTGATTGGATGGAAAAAGCCAGAA GGAACCATTAACTCCGTGGGATTTATGGACACACGAAAGCGACATCAGAGTGATGGCAATGAGATAGCCC ACACTAGGCTTCGAGCCTCAACCAGGGACCTGCAGGCATCCCCAAAGCCGACCTCCAAGTCTACCATTGA GGAAGATCTAAAGAAACTCATCGACCTTGAGAGCCCAACTCCCGAATCCCAGAAGAATTTCAAGTTCCAT GCACTGTCCTCCCCGCAGTCCCCGTTCCCCACTACCCCTACCTCCCGGCGGGCCCTGCACAGGACTCTGT CAGATGAGAGCATTTACAGCAGCCAGAGGGAGCATTTCTTCACCTCCAGGGCTTCGCTTCTAGACCAAGC CCTGCCCAACGATGTCCTCTTCAGCAGTACCTACCCATCTCTCCCCAAGTCACTTCCACTGAGGAGGCCA TCTTACACGTTGGGAATGAAGTCATTGCATGGAGAGTTCTCTGCCTCGGACAGCTCCCTCACCGACATCC AGGAGACCCGAAGGCAGCCTATCCCTGACCCTGGCCTGATGCCCCTGCCTGATGCAGCTTCAGATTTGGA CTGGTCCAACCTAGTAGATGCCGCCAAAGCCTATGAGGTCCAGAGAGCCTCATTTTTTGCTGCTAGTGAT GAAAACCATCGCCCCCTGAGCGCGGCCTCCAACAGTGACCAGCTGGAGGAGCAGGCCCTGGTCCAGATGA AGTCCTACAGCAGTAAGGACCCCTCTCCCACTCTGGCTTCTAAGGTGGACCAGCTGGAAGGTATGCTGAA AATGCTTCGAGAAGATTTGAAGAAGGTAAATGCTCTCAGAAAAAGAAGACAAGGCCCAGCTGCAGGCGGA AGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212077 representing BC058681 Red=Cloning site Green=Tags(s) MTSLKRSQTERPVTADRASVVSTDGAPKVHTDDFYMRRFRSQNGSLGSSVMAAVGPPRSEGPHHITSTPG VPKMGVRARIADWPPRKENVKESSRSSQEIETSSCLESLSSKGSPVSQGSSVSLNSNDSAMLKSIQNTLK NKTGPAESMDSRFLMPEAYPSSPRKALRRIRQRSNSDITISELDVDSFDECISPTYKSGPSLHREYGSTS SIDKQGTSGDSFFDLLKGYKDDRSDRGPTPTKLSDFLITGGGKGSGFSLDVIDGPISQRENLRLFKEREK PLKRRSKSETGDSSIFRKLRNAKGEELGKSSDLEDNRSEDSVRPWTCPKCFAHYDVQSILFDLNEAIMNR HNVIKRRNTTTGASAAAVASLVSGPLSHSASFSSPMGSTEDLNSKGSLGMDQGDDKSNELVMSCPYFRNE IGGEGERKISLSKSNSGSFSGCESTSFESALSSHCTNAGVAVLEVPKESLMLHLDRVKRYTVEHVDLGAY YYRKFFYQKEHWNYFGADENLGPVAVSIRREKPEDMKENGSPYNYRIIFRTSELMTLRGSVLEDAIPSTA KHSTARGLPLKEVLEHVIPELNVQCLRLAFNTPKVTEQLMKLDEQGLNYQQKVGIMYCKAGQSTEEEMYN NESAGPAFEEFLQLLGERVRLKGFEKYRAQLDTKTDSTGTHSLYTTYKDYEIMFHVSTMLPYTPNNKQQL LRKRHIGNDIVTIVFQEPGAQPFSPKNIRSHFQHVFVIVRAHNPCTESVCYSVAVTRSRDVPSFGPPIPK GVTFPKSNVFRDFLLAKVINAENAAHKSEKFRAMATRTRQEYLKDLAEKNVTNTPIDPSGKFPFISLASK KKEKSKPYPGAELSSMGAIVWAVRAKDYNKAMEFDCLLGISSEFIVLIEQETKSVAFNCSCRDVIGWTSS DTSLKIFYERGECVSVESFISGEDIKEIVRRLQFVSKGCESVEMTLRRNGLGQLGFHVNYEGIVADVEPY GYAWQAGLRQGSRLVEICKVAVATLSHEQMIDLLRTSVTVKVVIIPPHDDCTPRRSCSETYRMPVMEYQM NEGISYEFKFPFRNNNKWQRNASKGAHSPQVPSQLQSPMTSRLNAGKGDGKMPPPERAANIPRSISSDGR PLERRLSPGSDIYVTVSSMALARSQCRNSPSNLSSSSETGSGGGTYRQKSMPEGFGVSRRSPASIDRQNT QSDISGSGKSTPSWQRSEDSLADQMEPTCHLPAVSKVLPAFRESPSGRLMRQDPVVHLSPNKQGHSDSHY SSHSSSNTLSSNASSAHSDEKWYDGDRTESDLNSYNYLQGTSADSGIDTASYGPSHGSTASLGASTSSPR SGPGKEKVAPLWHSSSEVLSLADRTLETEGHGMDRKAESSLSLDIHSKSQGGSSPLSRENSTFSINDAAS HTSTMSSRHSASPVVFSSARSSPKEELHPTASSQLAPSFSSSSSSSSGPRTFYPRQGATSKYLIGWKKPE GTINSVGFMDTRKRHQSDGNEIAHTRLRASTRDLQASPKPTSKSTIEEDLKKLIDLESPTPESQKNFKFH ALSSPQSPFPTTPTSRRALHRTLSDESIYSSQREHFFTSRASLLDQALPNDVLFSSTYPSLPKSLPLRRP SYTLGMKSLHGEFSASDSSLTDIQETRRQPIPDPGLMPLPDAASDLDWSNLVDAAKAYEVQRASFFAASD ENHRPLSAASNSDQLEEQALVQMKSYSSKDPSPTLASKVDQLEGMLKMLREDLKKVNALRKRRQGPAAGG S TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC058681
ORF Size	5253 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058681, AAH58681
RefSeq Size	6195 bp
RefSeq ORF	5255 bp
Locus ID	217692
Gene Summary	Stimulates the GTPase activity of RAP2A. Promotes reorganization of the actin cytoskeleton and recruits DLG4 to F-actin. Contributes to the regulation of dendritic spine morphogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

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