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Klhl15 (NM_001039061) Mouse Tagged ORF Clone
CAT＃: MG213950
- TrueORF®
Klhl15 (tGFP-tagged) - Mouse kelch-like 15 (Drosophila) (Klhl15) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001039061" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330500C13Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG213950 representing NM_001039061
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCATCCATGACACCAGTGTCTCTGCTGGGTTCAGAGCAC TGTATGAGGAGGGATTGCTTCTCGATGTCACTCTGGTTATTGAAGACCATCAGTTCCAGGCCCATAAAGC TCTCTTGGCCACCCAGAGTGATTACTTCAGAATTATGTTCACAGCAGACATGAGGGAACGAGATCAGGAC AAAATTCATTTGAAAGGTCTAACAGCTACTGGTTTCAGCCACGTTCTTCAGTTTATGTATTATGGAACTA TAGAACTAAGTATGAACACTGTACATGAAATCCTTCAGGCAGCAATGTATGTTCAGCTTATAGAAGTGGT GAAATTCTGCTGCTCCTTTCTCTTAGCCAAAATCTGCTTAGAAAATTGTGCGGAAATTATGAGACTGTTA GATGATTTCGGTGTTAACATCGAGGGAGTCAGGGAGAAGCTGGATGCCTTCCTGCTAGACAACTTCGTGC CACTAATGTCCAGGCCTGACTTTTTGTCTTATCTGAGCTTCGAGAAGCTCATGTCTTATTTGGATAATGA CCATCTGAGCAGGTTTCCAGAAATTGAGCTGTACGAGGCTGTGCAGTCTTGGCTGCGACATGATAGACGA CGGTGGAGACACACAGATACCATCATTCAGAACATCAGGTTTTGTCTGATGACTCCATCCAGCGTCTTTG AGAAGGTTAAAACATCAGAATTTTATAGATACTCCCGACAGCTCCGATATGAAGTTGACCAAGCATTGAA TTACTTCCAAAATGTTCACCAGCAGCCCTTGTTGGACATGAAATCAAGCCGTATTCGTTCTGCCAAACCC CAAACTACAGTATTCCGAGGAATGATTGGACATAGCATGGTTAATAGCAAAATACTTCTGTTAAAAAAAC CAAGAGTCTGGTGGGAACTGGAGGGCCCCCAAGTACCTCTAAGGCCAGACTGCCTTGCTATTGTCAATAA TTTTGTATTCCTGCTAGGTGGAGAAGAACTGGGCCCTGATGGTGAATTCCATGCCTCTTCGAAAGTGTTC AGGTATGATCCAAGACAAAATTCTTGGCTGCGTATGGCAGACATGTCTGTACCCCGTTCAGAATTTGCTG TTGGTGTTATTGGAAAGTTTATTTATGCTGTAGCAGGCAGAACAAGAGATGAAACTTTCTACTCAACAGA AAGATATGATATCACTAATGATAAATGGGAATTTGTAGATCCTTATCCAGTTAACAAATATGGACATGAG GGGACAGTGCTCAATAACAAGTTGTTTATCACTGGAGGAATTACTTCATCTTCCACATCTAAACAGGTGT GTGTGTTTGACCCCAGTAAAGAAGGGACTATAGAGCAGCGGACCAGGAGAACGCAAGTAGTCACCAACTG CTGGGAGAATAAGAGCAAGATGAATTATGCCAGATGTTTTCACAAGATGATCTCTTACAACGGCAAGCTT TATGTCTTTGGTGGTGTCTGTGTGATCTTGAGGGCCTCTTTTGAATCTCAGGGCTGCCCTTCCACAGAGG TGTACAACCCAGATACCGATCAGTGGACCATCTTGGCCTCCATGCCAATTGGTAGAAGTGGTCATGGTGT GACTGTGCTGGACAAACAGATAATGGTCCTTGGAGGCCTTTGTTATAATGGTCATTACAGTGATTCCATT CTCACCTTTGACCCAGATGAAAATAAGTGGAAAGAAGATGAGTACCCTCGGATGCCCTGCAAGCTGGATG GTTTACAGGTGTGCAACCTACATTTTCCGGACTATGTACTGGATGAGGTTAGGCGTTGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG213950 representing NM_001039061
Red=Cloning site Green=Tags(s) MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQD KIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLL DDFGVNIEGVREKLDAFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRR RWRHTDTIIQNIRFCLMTPSSVFEKVKTSEFYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKP QTTVFRGMIGHSMVNSKILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVF RYDPRQNSWLRMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHE GTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCFHKMISYNGKL YVFGGVCVILRASFESQGCPSTEVYNPDTDQWTILASMPIGRSGHGVTVLDKQIMVLGGLCYNGHYSDSI LTFDPDENKWKEDEYPRMPCKLDGLQVCNLHFPDYVLDEVRRCN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039061 |
| ORF Size | 1812 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039061.1, NP_001034150.1 |
| RefSeq Size | 2363 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 236904 |
| UniProt ID | A2AAX3 |
| Gene Summary | Substrate-specific adapter for CUL3 E3 ubiquitin-protein ligase complex. Acts as an adapter for CUL3 to target the serine/threonine-protein phosphatase 2A (PP2A) subunit PPP2R5B for ubiquitination and subsequent proteasomal degradation, thus promoting exchange with other regulatory subunits and regulating PP2A holoenzyme composition. Acts as an adapter for CUL3 to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, plays a key role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219661 | Klhl15 (untagged) - Mouse kelch-like 15 (Drosophila) (Klhl15), transcript variant 1, (10ug) |
CNY 7,790.00 |
|
| MR213950 | Klhl15 (Myc-DDK-tagged) - Mouse kelch-like 15 (Drosophila) (Klhl15), transcript variant 1 |
CNY 4,496.00 |
|
| MR213950L3 | Lenti ORF clone of Klhl15 (Myc-DDK-tagged) - Mouse kelch-like 15 (Drosophila) (Klhl15), transcript variant 1 |
CNY 6,460.00 |
|
| MR213950L4 | Lenti ORF clone of Klhl15 (mGFP-tagged) - Mouse kelch-like 15 (Drosophila) (Klhl15), transcript variant 1 |
CNY 6,460.00 |
