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Asrgl1 (NM_025610) Mouse Tagged ORF Clone
CAT＃: MG220307
- TrueORF®
Asrgl1 (tGFP-tagged) - Mouse asparaginase like 1 (Asrgl1), (10ug)
ORF Plasmid: DDK
"NM_025610" in other vectors (4)
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CNY 3,520.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410004D18Rik; ALP; ALP1; AU040643; AW060375 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220307 representing NM_025610
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGCGCCCGCGGCACCGTGGCGCCGCCAGTCCGAGCCTCCATCGATGTGAGTCTCGTGGTGGTAG TCCATGGTGGCGGAGCAAGCAACATCTCAGCCAACCGGAAAGAGCTGGTGCGCGAGGGCATCGCCAGAGC GGCAACCGAGGGCTACAAAATCCTCAAGGCGGGAGGGAGCGCGGTAGATGCTGTGGAAGGAGCCGTGACT GTCCTGGAAAACGACCCAGAGTTCAACGCAGGTTATGGGTCTGTCCTGAATGTAAACGGTGATATTGAGA TGGATGCTAGTATCATGGATGGGAAGGACCTGTCTGCAGGTGCAGTGTCTGCCGTGCGCTGTATCGCAAA TCCCGTTAAACTTGCACGGCTTGTTATGGAAAAGACACCTCATTGCTTTCTGACTGGCCATGGTGCAGAG AAATTTGCAGAAGACATGGGGATTCCACAGGTCCCTGTAGAAAAACTGATAACTGAGAGAACCAAGAAGC ACCTGGAGAAAGAGAAGCTTGAGAAGGGAGCACAGAATGCTGACTGCCCTAAAAACTCAGGAACTGTGGG TGCTGTTGCCTTGGACTGCAGAGGAAACTTGGCTTACGCAACCTCTACTGGGGGGATTGTCAATAAAATG GTTGGCCGAGTTGGAGACTCGCCTTGCATAGGAGCTGGAGGTTACGCAGATAATAACCTTGGAGCCGTTT CAACCACAGGACATGGGGAAAGTATCCTGAAGGTGAATCTGGCCAGACTTGCCCTCTTCCATGTAGAGCA AGGAAAGACCGTAGAGGAGGCTGCTCAGTTGGCATTGGATTACATGAAGTCAAAACTCAAAGGTCTAGGT GGCCTCATCTTGGTCAACAAAACAGGAGACTGGGTGGCAAAGTGGACCTCTGCCTCCATGCCCTGGGCAG CGGTGAAGAATGGCAAGCTGCAGGCCGGCATTGACCTCTGTGAGACCAGGACAAGGGACCTACCTTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220307 representing NM_025610
Red=Cloning site Green=Tags(s) MACARGTVAPPVRASIDVSLVVVVHGGGASNISANRKELVREGIARAATEGYKILKAGGSAVDAVEGAVT VLENDPEFNAGYGSVLNVNGDIEMDASIMDGKDLSAGAVSAVRCIANPVKLARLVMEKTPHCFLTGHGAE KFAEDMGIPQVPVEKLITERTKKHLEKEKLEKGAQNADCPKNSGTVGAVALDCRGNLAYATSTGGIVNKM VGRVGDSPCIGAGGYADNNLGAVSTTGHGESILKVNLARLALFHVEQGKTVEEAAQLALDYMKSKLKGLG GLILVNKTGDWVAKWTSASMPWAAVKNGKLQAGIDLCETRTRDLPC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025610 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025610.3, NP_079886.2 |
| RefSeq Size | 2274 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 66514 |
| UniProt ID | Q8C0M9 |
| Gene Summary | Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210384 | Asrgl1 (untagged) - Mouse asparaginase like 1 (Asrgl1), (10ug) |
CNY 3,990.00 |
|
| MR220307 | Asrgl1 (Myc-DDK-tagged) - Mouse asparaginase like 1 (Asrgl1) |
CNY 3,230.00 |
|
| MR220307L3 | Lenti ORF clone of Asrgl1 (Myc-DDK-tagged) - Mouse asparaginase like 1 (Asrgl1) |
CNY 5,130.00 |
|
| MR220307L4 | Lenti ORF clone of Asrgl1 (mGFP-tagged) - Mouse asparaginase like 1 (Asrgl1) |
CNY 5,130.00 |
