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Ank1 (NM_001110783) Mouse Tagged ORF Clone
CAT＃: MG221878
- TrueORF®
Ank1 (tGFP-tagged) - Mouse ankyrin 1 erythroid (Ank1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001110783" in other vectors (2)
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CNY 22,990.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ank-1; nb; pale |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221878 representing NM_001110783
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAGGAGCAGAAGG AGAAGGAGGAATCCAACAGGAAGCGGCGGAACCGCTCCCGTGACCGCAAGAAGAAGGCCGATGCTGCTAC CAGCTTTCTGCGGGCGGCACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGAC ATTAACACCTGTAACCAGAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGG TGGTTGAACTTCTGCACAAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACAT CGCTGCCCTTGCTGGTCAGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAG TCTCAGAAAGGCTTTACTCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTAC TGGAGAATGGAGCCAATCAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCA GGGTCACGAGAATGTGGTGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTG CACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATG TGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTT GCTCCTCAACAGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCC CGCAGGGGGAACGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGG ATGAATTGACACCGCTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCA CGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCAC CTCGACTGTGTCCGACTTCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTC CTCTCCATGTGGCAGCCCACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCC CAACTCCAGAGCCCTGAATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATG GAGTTGCTGCTGAAGACAGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAG CCTCCTTCATGGGACACCTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAA TGTGAAAGTAGAAACCCCCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTC CAGAACAAAGCCAAAGCCAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCG GCCACACAGGCATGGTGAAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCA CACACCCCTACACACCGCAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCA TCCCAAGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGT TGGCGGAGCTGCTGCTGGAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCA TGTGGCCGTCCATCACAACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGC CCTGCCTGGAATGGCTACACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTC TACTGCAGTACGGAGGGTCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCA AGAGGGCCACACAGAAATGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGT GGACTCACTCCCCTCCACCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATG GCGTCACCGTGGATGCTACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACAT CAAGCTGGTGAAGTTCTTGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCC TTGCACCAGGCAGCTCAACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAA ATGAGGTCAGCTCGAACGGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGA CGTGCTCAAGGTGGTCACAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCT GAGACGGTGGACGAGATCCTGGACGTGTCTGAAGATGAAGGAACTGCTCACATATCTATAATGGGTGACG AACTTGTCGGCTCGAAGGCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTT TGTGCCGAAGCTAGACCAAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACC GTTGTGATCCGATCTGAAGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCA GCCCGGCCACAGAGACCTCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAG CTTCATGGTGGATGCCCGAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCT CCCCGGACATGCGCAGCACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCAC CCCCACTGGCTGAGGAAGAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCT TAGCCCTGTGATCGTGGAAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTG AGGAGTGAAAACGGCTCTGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCC TTAATGGCATGGATGAAGAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCAC CACCGACTTCCCTCTGTACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAA GGGGGTTCCCTGAGAAGCAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGA AAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGC CACATTCAGCCCCATTGTGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATC CCGCTCCCTCCCTCATGGACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTAC TGTGTAGCGTCATCGGTGGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATA CGCCAATGAGTGTGCCAACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACT GCGGAGGCTGTGCACTTTGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTG TCATTTTTGCCAAGATGAACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGT GGACAAGACCCTGGAGCAGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAA GGGATGCCTCTGTTTGCAGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCT TCCATTTCCAGTCGTTTCGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCC AGGAGGGTTCTTGTCGTTCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTG AATATCACCATGCCCCCCTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGA CCCTTCGATACAGCATTCTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAG GATGGCTGTCATCAGAGAACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTG GAAGACATCAACCGGATCCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCC TCTGGGTGGACCGTGAAGGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAG GAGTGAGATTGTTAACATGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACAT GGGGACCGGGAGTACTCATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGT CCCCCGCCTCCCTGCAGTACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGT CATAGACGCCATCCCCCTGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGG TCTGCGGGCCTCACACCCTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGAGGACT CTGACGCCATACCAGAGTGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTC TCAGGACCTGGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGTCAG GAGGAAGGTCAGAGGTCAGAAAAGAAGAGGCAGGAAGTCTCAGGCACAGAGCAGGACACGGAGACTGAAG TGTCTCTTGTTTCAGGCCAGCAGCGAGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAGGTGCT GGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAGAAGCC ACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCACCATCC AAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTGCAGAG GGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCGCCTTC TCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATTCACAG ATGAACAGGGCAACATTGTTACCAAGAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCTGGTGC CATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAGAGGGC AGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGCAAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221878 representing NM_001110783
Red=Cloning site Green=Tags(s) MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVD INTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQ SQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPAL HIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIAS RRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDH LDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVM ELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL QNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEA SQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS PAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKS GLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSP LHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYP ETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPET VVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIP PRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVL RSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPE GGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRI PLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRT AEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLE GMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHL NITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSV EDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRH GDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVW SAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQ EEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEA TQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAF SQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEG RKGAQIVKRASLKRGKQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001110783 |
| ORF Size | 5721 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001110783.3, NP_001104253.1 |
| RefSeq Size | 8218 bp |
| RefSeq ORF | 5724 bp |
| Locus ID | 11733 |
| UniProt ID | Q02357 |
| Gene Summary | Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[UniProtKB/Swiss-Prot Function] |
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