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Pcsk5 (NM_001190483) Mouse Tagged ORF Clone
CAT＃: MG222006
- TrueORF®
Pcsk5 (tGFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001190483" in other vectors (2)
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CNY 20,620.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | b2b585C; b2b585Clo; b2b1549C; b2b1549Clo; PC; PC5; PC5/; PC6; SP; SPC6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222006 representing NM_001190483
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGGACTGGGGGAACCGCTGCAGCCGCCCGGGACGGCGGGACCTGCTGTGCGTGCTGGCACTGC TCGCCGGCTGTCTGCTCCCGGTATGCCGGACGCGCGTCTACACCAACCACTGGGCAGTGAAGATCGCCGG CGGCTTCGCGGAGGCAGATCGCATAGCCAGCAAGTACGGATTCATCAACGTAGGACAGATCGGTGCACTG AAGGACTACTATCACTTCTACCATAGTAGGACCATTAAAAGGTCTGTTCTCTCGAGCAGAGGAACCCACA GTTTCATTTCAATGGAACCAAAGGTGGAGTGGATCCAACAGCAAGTGGTGAAAAAAAGAACCAAGAGGGA TTATGACCTCAGCCATGCCCAGTCAACCTACTTCAATGATCCCAAGTGGCCAAGTATGTGGTACATGCAC TGCAGTGACAACACCCATCCTTGCCAGTCAGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTACACGG GGAAGAATATCGTGGTGACTATCCTGGATGACGGCATCGAGAGAACCCACCCAGATCTGATGCAAAACTA CGATGCTCTGGCAAGTTGCGATGTGAATGGGAATGACTTGGACCCGATGCCTCGTTATGATGCAAGCAAT GAGAACAAGCATGGGACCCGCTGTGCCGGAGAAGTGGCAGCCACTGCAAACAACTCTCACTGCACTGTCG GGATCGCTTTCAACGCCAAGATTGGAGGTGTGAGAATGCTGGATGGTGATGTCACTGACATGGTGGAGGC AAAGTCTGTCAGCTACAACCCACAGCATGTGCACATCTACAGTGCCAGCTGGGGACCAGATGACGATGGC AAGACTGTGGATGGGCCAGCTCCCCTCACCCGGCAAGCCTTTGAGAACGGCGTGAGAATGGGGCGGAGAG GCCTTGGATCTGTGTTTGTGTGGGCATCTGGCAATGGTGGACGGAGCAAGGATCACTGTTCTTGTGATGG CTATACCAACAGCATCTATACCATCTCCATCAGCAGTACGGCCGAAAGTGGAAAGAAACCTTGGTACTTG GAAGAGTGTTCATCTACACTGGCTACAACCTACAGCAGTGGAGAATCCTATGATAAGAAAATAATCACTA CTGATCTAAGGCAGCGATGCACAGACAATCACACTGGAACATCAGCCTCAGCCCCCATGGCTGCTGGAAT CATTGCCCTGGCCCTAGAAGCCAATCCGTTTCTGACCTGGAGAGACGTGCAGCATGTTATTGTCAGGACT TCCCGTGCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCATCTCT ATGGATTTGGACTGATGGATGCCGAAGCCATGGTGATGGAAGCAGAGAAGTGGACAACTGTTCCTCAGCA GCACGTGTGTGTGGAAAGCACAGACCGACAAATCAAGACCATTCGACCAAACAGTGCAGTGCGCTCCATC TACAAAGCCTCAGGCTGCTCGGATAATCCCAACCATCACGTCAATTACCTGGAGCATGTAGTTGTGCGTA TTACCATCACACACCCACGGAGGGGAGACCTGGCCATCTATCTGACATCACCCTCAGGAACCAGATCCCA GCTCTTGGCCAACAGGCTCTTTGATCATTCCATGGAAGGGTTTAAGAACTGGGAGTTCATGACTATTCAT TGCTGGGGAGAACGGGCTGCTGGGGACTGGGTCCTCGAAGTTTATGATACGCCATCTCAGCTGAGGAACT TCAAGACTCCAGGTAAATTGAAAGAATGGTCCTTAGTCCTCTATGGCACGTCCGTACAGCCATACTCCCC AACCAACGAGTTTCCCAAAGTGGAACGCTTCCGCTACAGCCGAGTGGAAGACCCCACAGATGACTACGGT GCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTGACGGGCCAGGACCAGATC ACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAATCTGTGTCTCCAGCTGCCC TCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAACTGCGAGTCCTGCTTTGGC AGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAGAAACTAGCAGCTGTGTTA CTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAAATGCAGTGAGAACTGCAA GGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTTCAGGGATCCCGCTGTTCG GTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCCATCGCTTCTGTGCTACTT GCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCATGGAAGAGGGAAGGTGTGT ACAAAGTTGTAGTGTGAGCTACTACTTGGACCACTCTTCAGAGGGTGGCTACAAATCCTGCAAGAGATGT GATAACAGCTGTTTGACATGCAATGGGCCAGGATTCAAGAACTGTTCCAGCTGCCCCAGTGGATATCTTT TAGACTTAGGAACGTGTCAGATGGGAGCCATCTGCAAGGATGGAGAATACATTGATGACCAAGGCCACTG CCAAACCTGTGAAGCCTCATGTGCCAAGTGCTGGGGACCAACTCAGGAAGACTGTATCAGCTGCCCCGTA ACAAGGGTCTTGGATGATGGTCGCTGTGTTATGAACTGTCCTTCCTGGAAGTTCGAATTTAAGAAGCAAT GCCATCCCTGCCACTACACTTGTCAAGGATGCCAAGGTAGTGGCCCTTCCAACTGCACCTCCTGTAGAGC AGACAAGCATGGTCAGGAGCGCTTCCTGTACCACGGGGAATGTCTGGAGAACTGCCCTGTGGGCCATTAT CCTGCCAAGGGACACGCCTGCCTACCCTGCCCAGACAACTGTGAGCTTTGCTACAACCCACACGTCTGCA GTCGATGCATGAGTGGCTATGTCATCATTCCCCCCAACCACACCTGCCAGAAGCTGGAGTGCAGACAAGG TGAATTCCAGGATTCTGAGTATGAAGAATGCATGCCCTGTGAGGAAGGATGTCTGGGATGCACTGAGGAT GATCCAGGAGCCTGTACCTCGTGTGCTACAGGATATTACATGTTTGAGCGGCATTGCTATAAAGCCTGTC CTGAGAAGACCTTCGGTGTGAAATGGGAATGCAGGGCCTGTGGTACTAACTGTGGCAGCTGCGACCAACA TGAGTGCTACTGGTGTGAGGAGGGCTTCTTTCTCTCCGGTGGCAGCTGTGTGCAGGATTGTGGCCCTGGC TTCCATGGAGACCAAGAGTTGGGAGAATGCAAGCCCTGCCACCGAGCCTGTGAGAATTGCACTGGCTCGG GCTACAACCAATGCAGCAGCTGTCAAGAAGGGTTGCAGCTATGGCATGGGACGTGTCTCTGGTCCACCTG GCCTCAGGTGGAAGGCAAGGACTGGAATGAAGCCGTGCCCACTGAAAAGCCATCTTTGGTGAGGAGTCTG CTGCAGGATCGACGAAAGTGGAAAGTTCAAATCAAAAGAGATGCAACGAGCCAGAATCAACCTTGTCACT CTTCTTGTAAAACCTGCAATGGATCTCTCTGCGCTTCATGTCCCACAGGTATGTACCTGTGGCTGCAGGC CTGTGTTCCTTCCTGTCCCCAAGGCACTTGGCCATCAGTCACCAGTGGCAGCTGTGAAAAGTGTTCCGAG GACTGTGTCTCCTGCTCCGGTGCCGACCTTTGCCAACAGTGCCTGAGCCAGCCGGACAACACTCTGCTTC TCCATGAGGGCAGGTGCTACCACAGTTGCCCAGAGGGCTTTTATGCAAAAGATGGTGTTTGTGAACACTG TAGTTCCCCTTGCAAAACATGCGAAGGAAATGCCACCAGCTGTAACTCTTGTGAAGGAGACTTCGTCCTA GACCATGGGGTGTGCTGGAAAACTTGCCCTGAAAAGCACGTGGCCGTGGAAGGAGTCTGCAAGCACTGTC CAGAGAGGTGCCAGGACTGCATCCACGAGAAAACTTGCAAAGAGTGCATGCCTGACTTCTTTCTATACAA TGACATGTGCCATCGTTCCTGTCCCAAGAGCTTCTACCCTGACATGCGCCAGTGTGTCCCCTGCCACAAA AACTGTCTGGAGTGCAATGGCCCCAAGGAAGATGACTGTAAGGTCTGTGCTGATACTTCTAAGGCTCTCC ACAATGGATTGTGCTTGGATGAGTGCCCTGAGGGAACCTACAAAGAAGAAGAGAATGATGAATGCAGAGA TTGCCCCGAGTCCTGCCTGATCTGCTCATCAGCTTGGACCTGCCTGGCCTGTCGGGAAGGCTTCACAGTA GTGCATGATGTCTGCACGGCACCCAAGGAGTGTGCAGCCGTCGAGTACTGGGATGAGGGCTCCCACAGGT GCCAGCCGTGTCACAAGAAATGCTCCCGCTGCTCAGGGCCTTCTGAGGACCAGTGCTATACCTGTCCCAG AGAGACTTTTCTTCTCAATACAACCTGTGTGAAAGAGTGCCCAGAGGGCTACCACACTGACAAGGACAGC CAGCAGTGTGTGCTCTGCCACAGCTCTTGCAGGACCTGCGAAGGACCACACAGCATGCAGTGCCTCTCCT GCCGACCTGGCTGGTTTCAACTGGGCAAGGAGTGCTTGCTGCAATGCAGGGATGGATATTATGGAGAAAG CACCAGCGGTAGGTGTGAGAAGTGTGACAAGAGCTGCAAGTCATGCCGAGGTCCACGGCCCACAGACTGC CAGTCTTGCGATACATTTTTCTTTCTCCTACGCTCCAAGGGACAGTGCCACCGCGCTTGCCCTGAGCACT ACTATGCAGACCAACACGCCCAGACCTGTGAGAGGTGCCACCCCACTTGTGACAAGTGCAGCGGAAAGGA GGCGTGGAGTTGCTTGTCCTGTGTGTGGAGCTACCACCTTCTGAAAGGAATCTGCATCCCAGAATGTATC GTAGGGGAATACAGAGAGGGAAAGGGAGAAAACTTTAACTGTAAAAAATGCCACGAGAGCTGCATGGAAT GCAAGGGTCCAGGCAGCAAGAACTGCACCGGGTGCTCAGCTGGCCTGCTGCTGGACATGGACGACAACCG CTGCCTCCATTGCTGCAATGCCTCCCACTCCCGCAGATCCCAGGACTGCTGCGACTGCCAGAGTTCCACA GATGAGTGCATCCTTCCAGCCAGGGAGGCTGAGTTTTACGAGCACACCAAGACTGCTCTGCTAGTGACCT CTGGCGCCATGCTGTTGCTGCTGCTGGGGGCTGCTGCGGTCGTGTGGCGGAAGTCTCGAAGCAGACCTGT GGCAAAGGGGCGGTACGAAAAGCTGGCAGAACCTACCGTGTCATACTCCTCCTACAGGAGCAGCTATCTT GACGAGGACCAGGTGATTGAGTACAGGGACCGGGACTACGATGAGGACGATGAGGACGACATCGTCTATA TGGGCCAAGATGGCACTGTCTACCGGAAGTTCAAGTATGGGCTGCTGGATGAGACGGAAGATGATGAGTT GGAGTACGATGATGAGAGCTACTCTTACCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222006 representing NM_001190483
Red=Cloning site Green=Tags(s) MDWDWGNRCSRPGRRDLLCVLALLAGCLLPVCRTRVYTNHWAVKIAGGFAEADRIASKYGFINVGQIGAL KDYYHFYHSRTIKRSVLSSRGTHSFISMEPKVEWIQQQVVKKRTKRDYDLSHAQSTYFNDPKWPSMWYMH CSDNTHPCQSDMNIEGAWKRGYTGKNIVVTILDDGIERTHPDLMQNYDALASCDVNGNDLDPMPRYDASN ENKHGTRCAGEVAATANNSHCTVGIAFNAKIGGVRMLDGDVTDMVEAKSVSYNPQHVHIYSASWGPDDDG KTVDGPAPLTRQAFENGVRMGRRGLGSVFVWASGNGGRSKDHCSCDGYTNSIYTISISSTAESGKKPWYL EECSSTLATTYSSGESYDKKIITTDLRQRCTDNHTGTSASAPMAAGIIALALEANPFLTWRDVQHVIVRT SRAGHLNANDWKTNAAGFKVSHLYGFGLMDAEAMVMEAEKWTTVPQQHVCVESTDRQIKTIRPNSAVRSI YKASGCSDNPNHHVNYLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANRLFDHSMEGFKNWEFMTIH CWGERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSVQPYSPTNEFPKVERFRYSRVEDPTDDYG AEDYAGPCDPECSEVGCDGPGPDHCSDCLHYYYKLKNNTRICVSSCPPGHYHADKKRCRKCAPNCESCFG SHGNQCLSCKYGYFLNEETSSCVTQCPDGSYEDIKKNVCGKCSENCKACIGFHNCTECKGGLSLQGSRCS VTCEDGQFFNGHDCQPCHRFCATCSGAGADGCINCTEGYVMEEGRCVQSCSVSYYLDHSSEGGYKSCKRC DNSCLTCNGPGFKNCSSCPSGYLLDLGTCQMGAICKDGEYIDDQGHCQTCEASCAKCWGPTQEDCISCPV TRVLDDGRCVMNCPSWKFEFKKQCHPCHYTCQGCQGSGPSNCTSCRADKHGQERFLYHGECLENCPVGHY PAKGHACLPCPDNCELCYNPHVCSRCMSGYVIIPPNHTCQKLECRQGEFQDSEYEECMPCEEGCLGCTED DPGACTSCATGYYMFERHCYKACPEKTFGVKWECRACGTNCGSCDQHECYWCEEGFFLSGGSCVQDCGPG FHGDQELGECKPCHRACENCTGSGYNQCSSCQEGLQLWHGTCLWSTWPQVEGKDWNEAVPTEKPSLVRSL LQDRRKWKVQIKRDATSQNQPCHSSCKTCNGSLCASCPTGMYLWLQACVPSCPQGTWPSVTSGSCEKCSE DCVSCSGADLCQQCLSQPDNTLLLHEGRCYHSCPEGFYAKDGVCEHCSSPCKTCEGNATSCNSCEGDFVL DHGVCWKTCPEKHVAVEGVCKHCPERCQDCIHEKTCKECMPDFFLYNDMCHRSCPKSFYPDMRQCVPCHK NCLECNGPKEDDCKVCADTSKALHNGLCLDECPEGTYKEEENDECRDCPESCLICSSAWTCLACREGFTV VHDVCTAPKECAAVEYWDEGSHRCQPCHKKCSRCSGPSEDQCYTCPRETFLLNTTCVKECPEGYHTDKDS QQCVLCHSSCRTCEGPHSMQCLSCRPGWFQLGKECLLQCRDGYYGESTSGRCEKCDKSCKSCRGPRPTDC QSCDTFFFLLRSKGQCHRACPEHYYADQHAQTCERCHPTCDKCSGKEAWSCLSCVWSYHLLKGICIPECI VGEYREGKGENFNCKKCHESCMECKGPGSKNCTGCSAGLLLDMDDNRCLHCCNASHSRRSQDCCDCQSST DECILPAREAEFYEHTKTALLVTSGAMLLLLLGAAAVVWRKSRSRPVAKGRYEKLAEPTVSYSSYRSSYL DEDQVIEYRDRDYDEDDEDDIVYMGQDGTVYRKFKYGLLDETEDDELEYDDESYSYQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190483 |
| ORF Size | 5631 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190483.2, NP_001177412.1 |
| RefSeq Size | 6675 bp |
| RefSeq ORF | 5634 bp |
| Locus ID | 18552 |
| UniProt ID | Q04592 |
| Gene Summary | This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015] |
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