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Ptpn14 (NM_008976) Mouse Tagged ORF Clone
CAT＃: MG222223
- TrueORF®
Ptpn14 (tGFP-tagged) - Mouse protein tyrosine phosphatase non-receptor type 14 (Ptpn14), (10ug)
ORF Plasmid: DDK
"NM_008976" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C130080N23Rik; OTTMUSG00000022087; PTP36 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222223 representing NM_008976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTCGGCCTGAAGCTCCGCAGGACTCGGCGCTACAACGTCCTGAGCAAGAACTGCTTTGTTACCC GGATCCGCCTGCTGGACAGCAATGTCATCGAGTGCACGCTGTCGGTGGAAAGCACGGGGCAAGAGTGCCT GGAGGCCGTGGCCCAGAGGCTGGAGCTGAGGGAGACGCACTACTTCGGCCTTTGGTTTCTCAGCAAGAGC CAGCAGGCGAGATGGGTAGAGCTGGAGAAGCCACTGAAGAAACATCTGGACAAGTTTGCTAACGAGCCTC TGCTTTTCTTCGGAGTCATGTTCTATGTGCCAAATGTGTCACGGCTTCAGCAGGAGGCCACAAGATATCA GTATTACCTGCAAGTCAAAAAAGACGTGCTTGAAGGACGGTTGCGGTGCTCCCTGGAACAAGTGATCCGG CTGGCTGGCTTAGCTGTGCAAGCTGACTTCGGAGATTATAACCAGTTTGATTCCCAAGAGTTCCTCCGAG AGTATGTGCTCTTTCCTATGGATTTGGCCATGGAGGAGGCGGCTCTGGAGGAGCTAACCCAGAAGGTGGC CCAGGAACACAAAGCTCATAGCGGGATCCTGCCGGCTGAAGCTGAACTGATGTACATCAACGAGGTAGAG CGTTTGGATGGATTTGGACAGGAGATCTTCCCCGTGAAGGACAGTCATGGCAACAGCGTGCACCTCGGCA TCTTCTTCATGGGGATTTTTGTGAGGAACAGGGTCGGGAGACAGGCAGTGATATACAGGTGGAATGACAT TGGGAGTGTTACTCACAGCAAAGCAGCCATCCTGTTGGAGCTGATTGACAAGGAGGAGACCGCGCTCTTC CATACAGATGATATTGAAAATGCCAAGTACATTTCTCGGTTGTTTACCACTCGGCACAAATTTTACAAAC AGAACAAGATCTGCACTGAACAGTCAAATTCTCCACCCCCAATTAGACGCCAGCCCACCTGGAGCCGGTC CTCACTGCCAAGGCAGCAGCCGTATATCTTGCCTCCCATGCATGTCCAGTGCAGTGAGCACTACTCGGAG ACCCATACTTCCCAAGACAGCATTTTCCCCGGGAACGAAGAAGCCTTGTACTGCCGTTCTCACAACAGCC TGGACCTTAATTACTTGAACGGCACCGTCACCAATGGCAGCGTGTGCAGCGTTCACAGCGTCAACTCCCT CAGCTGCTCTCAGAGCTTCATCCAGGCGTCTCCAGTGTCCTCCAACCTTAGCATCCCTGGGAGTGACATC ATGAGGGCCGATTACATCCCCAGCCACCGCCACAGCACCATCATCGTGCCGTCTTACAGGCCGACCCCAG ATTACGAGACGGTCATGAGGCAGATGAAGAGGGGTCTGATGCACGCAGACAGCCAGAGCCGGTCTCTGCG TAACCTCAATATCATCAACACCCATGCCTATAACCAGCCCGAGGAACTGGTGTACAGCCAGCCGGAGATG CGGGAGAGGCATCCCTACACGGTCCCCTATGCACACCAGGGGTGCTACGGTCACAAACTTGTAAGTCCGT CTGACCAGATGAACCCCCAAAATTGTGCGATGCCTATCAAGCCAGGGGCCAGTTCCATCTCACACACAGT GAGCACTCCAGAACTAGCCAACATGCAGCTCCAAGGAGCACAACACTATAGCACAGCCCACATGCTCAAG AACTATCTATTCAGGCCGCCACCCCCTTACCCTCGGCCCCGTCCTGCCACCAGCACCCCAGACCTCGCCA GCCACCGCCACAAGTACGTCAGCGGCAGCAGCCCTGATCTGGTAACTCGGAAGGTGCAGCTCTCCGTAAA GACCTTCCAGGAGGACAGCTCACCTGTGGTCCATCAGTCTCTGCAGGAGGTGAGCGAACCCCTTACAGCC ACCAAGCACCATGGCGGCGGCGGTGGCACGGTGAATAAACGCCACAGCCTGGAGGTGATGAACAGCATGG TGAGAGGCATGGAGGCCATGACACTGAAGTCACTCAATATCCCCATGGCTCGCCGCAACACCCTTCGGGA GCAGGGCCCTTCCGAGGAGACGGGCGGCCACGAAGTGCACGGTCTCCCCCAGTATCACCACAAGAAGACA TTCTCGGATGCCACCATGCTGATCCACAGCAGTGAGAGCGAGGAAGAGGAGGAGACCCTGGAGGCTGCAC CTCAGGTTCCTGTGCTTCGAGAGAAAGTAGAATACAGTGCCCAGCTGCAGGCTGCCCTGGCCCGCATCCC CAACAGGCCCCCACCTGAGTACCCAGGGCCAAGAAAAAGTGTCAGTAATGGGGCACTGAGACAGGACCAG GGAACCCCTCTTCCTGCCATGGCCAGGTGCAGGGTGCTGAGACACGGACCATCCAAGGCCCTCAGTGTCT CCCGGGCAGAGCAGCTGGCTGTCAACGGTGCCTCTCTGGGTCCCTCCATCTCTGAGCCTGACCTAACCAG CGTGAAGGAGCGGGTCAAGAAAGAGCCTGTGAAGGAAAGGCCGGTGTCAGAGATGTTCTCCCTGGAGGAC AGCATTATAGAGAGAGAGATGATGATCAGGAATCTAGAGAAGCAGAAGATGACGGGCCCGCAGGCACAGA AGAGACCGCTGATGTTGGCAGCGCTGAATGGGCTCTCGGTGGCCCGAGTGTCGGGGCGGGAAGACGGTCA CCATGATGCCACCCGAGTCCCCATAGACGAGAGGCTCAGAGCCCTGAAGAAGAAGCTGGAAGATGGAATG GTGTTCACAGAATATGAGCAGATTCCAAACAAAAAGGCCAACGGCGTCTTCAGCACCGCCACTCTGCCTG AGAATGCCGAGCGCAGCCGGATCCGAGAAGTTGTCCCATACGAGGAGAATCGAGTGGAGCTCATCCCGAC CAAAGAAAACAACACAGGCTATATCAACGCCTCCCACATCAAGGTGGTGGTCGGCGGATCAGAATGGCAC TACATCGCCACCCAGGGGCCCTTGCCACATACGTGCCATGACTTCTGGCAGATGGTGTGGGAGCAGGGGG TGAATGTGATCGCCATGGTCACTGCAGAGGAGGAGGGTGGACGGACCAAAAGCCATCGATACTGGCCCAA ACTGGGGTCCAAGCATAGTTCTGCCACCTACGGCAAGTTCAAGGTCACCACAAAGTTCCGGACAGATTCT GGTTGCTATGCAACGACGGGCCTAAAGGTGAAGCACCTGCTGTCCGGGCAGGAGAGGACCGTGTGGCACT TGCAGTACACGGACTGGCCCCACCACGGCTGTCCAGAAGACGTCCAAGGATTTTTGTCCTACTTGGAGGA AATCCAGTCAGTCCGACGCCACACCAACAGCGTGCTGGAAGGCATCAGGACCAGGCACCCCCCCATCGTG GTTCACTGCAGCGCGGGTGTGGGAAGGACTGGTGTGGTTATCCTCTCTGAGCTCATGATCTACTGCCTGG AACACAACGAAAAGGTGGAGGTGCCCACGATGCTGCGATTCCTCAGGGAGCAGAGGATGTTCATGATCCA GACCATTGCGCAGTACAAGTTCGTCTACCAAGTCCTCGTCCAGTTCCTGCAGAATTCCAGGCTCATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222223 representing NM_008976
Red=Cloning site Green=Tags(s) MPFGLKLRRTRRYNVLSKNCFVTRIRLLDSNVIECTLSVESTGQECLEAVAQRLELRETHYFGLWFLSKS QQARWVELEKPLKKHLDKFANEPLLFFGVMFYVPNVSRLQQEATRYQYYLQVKKDVLEGRLRCSLEQVIR LAGLAVQADFGDYNQFDSQEFLREYVLFPMDLAMEEAALEELTQKVAQEHKAHSGILPAEAELMYINEVE RLDGFGQEIFPVKDSHGNSVHLGIFFMGIFVRNRVGRQAVIYRWNDIGSVTHSKAAILLELIDKEETALF HTDDIENAKYISRLFTTRHKFYKQNKICTEQSNSPPPIRRQPTWSRSSLPRQQPYILPPMHVQCSEHYSE THTSQDSIFPGNEEALYCRSHNSLDLNYLNGTVTNGSVCSVHSVNSLSCSQSFIQASPVSSNLSIPGSDI MRADYIPSHRHSTIIVPSYRPTPDYETVMRQMKRGLMHADSQSRSLRNLNIINTHAYNQPEELVYSQPEM RERHPYTVPYAHQGCYGHKLVSPSDQMNPQNCAMPIKPGASSISHTVSTPELANMQLQGAQHYSTAHMLK NYLFRPPPPYPRPRPATSTPDLASHRHKYVSGSSPDLVTRKVQLSVKTFQEDSSPVVHQSLQEVSEPLTA TKHHGGGGGTVNKRHSLEVMNSMVRGMEAMTLKSLNIPMARRNTLREQGPSEETGGHEVHGLPQYHHKKT FSDATMLIHSSESEEEEETLEAAPQVPVLREKVEYSAQLQAALARIPNRPPPEYPGPRKSVSNGALRQDQ GTPLPAMARCRVLRHGPSKALSVSRAEQLAVNGASLGPSISEPDLTSVKERVKKEPVKERPVSEMFSLED SIIEREMMIRNLEKQKMTGPQAQKRPLMLAALNGLSVARVSGREDGHHDATRVPIDERLRALKKKLEDGM VFTEYEQIPNKKANGVFSTATLPENAERSRIREVVPYEENRVELIPTKENNTGYINASHIKVVVGGSEWH YIATQGPLPHTCHDFWQMVWEQGVNVIAMVTAEEEGGRTKSHRYWPKLGSKHSSATYGKFKVTTKFRTDS GCYATTGLKVKHLLSGQERTVWHLQYTDWPHHGCPEDVQGFLSYLEEIQSVRRHTNSVLEGIRTRHPPIV VHCSAGVGRTGVVILSELMIYCLEHNEKVEVPTMLRFLREQRMFMIQTIAQYKFVYQVLVQFLQNSRLI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008976 |
| ORF Size | 3567 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008976.2, NP_033002.2 |
| RefSeq Size | 10766 bp |
| RefSeq ORF | 3570 bp |
| Locus ID | 19250 |
| UniProt ID | Q62130 |
| Gene Summary | Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223884 | Ptpn14 (untagged) - Mouse protein tyrosine phosphatase, non-receptor type 14 (Ptpn14), (10ug) |
CNY 15,300.00 |
|
| MR222223 | Ptpn14 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 14 (Ptpn14) |
CNY 8,280.00 |
|
| MR222223L3 | Lenti ORF clone of Ptpn14 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 14 (Ptpn14) |
CNY 11,500.00 |
|
| MR222223L4 | Lenti ORF clone of Ptpn14 (mGFP-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 14 (Ptpn14) |
CNY 10,680.00 |
