United States
Germany
Japan
United Kingdom
China
Piwil2 (NM_021308) Mouse Tagged ORF Clone
CAT＃: MG223877
- TrueORF®
Piwil2 (tGFP-tagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2), (10ug)
ORF Plasmid: DDK
"NM_021308" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mili; Piwil1l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223877 representing NM_021308
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCTGTCAGGCCGTTGTTCAGGGGGCCCACCCCAGTCCACCCATCTCAGTGTGTGCGGATGCCAG GCTGTTGGCCTCAAGCTCCTAGACCTTTGGAACCAGCTTGGGGTAGGGCAGGACCTGCAGGCAGAGGCCT TGTGTTTAGAAAACCAGAAGACTCCAGCCCACCACTCCAGCCAGTACAAAAGGATTCTGTAGGTTTGGTG TCCATGTTCCGTGGCATGGGTCTTGACACAGCATTCCGGCCTCCTTCAAAACGAGAAGTGCCTCCTTTAG GCAGAGGAGTTCTAGGTCGAGGCTTGTCTGCTAACATGGTCCGCAAGGACAGAGAAGAACCCCGTTCCTC TTTGCCTGATCCTTCGGTGCTGGCAGCTGGGGACAGCAAACTGGCAGAGGCTTCTGTTGGTTGGAGTAGA ATGCTGGGAAGAGGTAGTTCTGAGGTCTCTCTGTTACCACTGGGACGAGCAGCCAGTAGTATAGGCAGAG GAATGGACAAACCTCCCAGTGCCTTTGGCCTCACAGCTCGGGATCCCCCACGGCTGCCACAGCCTCCAGC TCTGTCTCCAACTTCACTGCACTCTGCCGATCCCCCTCCGGTCCTGACTATGGAACGAAAGGAAAAAGAG CTTTTGGTCAAGCAAGGATCAAAAGGAACTCCTCAGTCTTTGGGACTGAACCTCATCAAAATCCAGTGTC ATAACGAAGCAGTTTATCAGTACCATGTGACTTTCAGCCCCAGTGTGGAATGCAAAAGCATGAGGTTTGG CATGTTGAAGGACCACCAGTCTGTCACTGGAAACGTCACTGCTTTTGATGGCTCTATTCTTTATCTTCCT GTTAAGCTTCAACAAGTTGTTGAGTTAAAAAGTCAGAGGAAAACTGACGATGCCGAGATCAGTATCAAGA TTCAGCTGACAAAGATCCTGGAGCCGTGTTCTGACCTGTGCATCCCCTTCTACAATGTTGTCTTCCGGCG GGTAATGAAACTTCTGGATATGAAGCTTGTGGGGAGAAACTTCTATGACCCTACAAGTGCCATGGTACTG CAGCAACACAGATTGCAGATCTGGCCTGGCTATGCGGCTAGTATCCGGAGGACAGACGGGGGTCTCTTCC TGCTCGCTGATGTCTCTCATAAGGTCATTCGGAACGACTCTGTGCTGGATGTCATGCATGCTATCTACCA GCAGAACAAGGAGCACTTCCAGGACGAGTGCAGCAAGCTTCTGGTTGGCAGCATTGTCATCACGCGCTAC AACAATCGTACCTACCGAATCGATGATGTGGACTGGAACAAGACCCCTAAAGACAGCTTTGTCATGTCGG ACGGGAAGGAAATCACATTCCTGGAATACTACAGCAAAAACTATGGGATCACAGTCAAGGAAGATGACCA GCCGCTGCTGATCCACCGGCCCAGTGAGAGACAGAATAACCATGGCATGTTGCTGAAGGGCGAGATCCTG CTGCTGCCCGAGCTCTCCTTCATGACGGGGATCCCTGAGAAGATGAAGAAGGACTTCAGGGCCATGAAGG ACTTGACTCAGCAGATTAACCTGAGCCCCAAGCAGCACCACGGTGCTTTGGAATGCCTGCTGCAGAGAAT TTCACAAAACGAGACAGCCAGCAATGAGCTGACCCGCTGGGGGCTCAGTCTGCATAAAGATGTCCACAAG ATTGAAGGTCGGCTTCTGCCAATGGAGAGGATCAACTTAAGGAACACTTCATTTGTCACATCGGAGGACC TGAACTGGGTTAAGGAAGTGACCAGAGATGCTTCCATTCTAACTATTCCCATGCATTTCTGGGCACTCTT TTATCCAAAGAGAGCAATGGACCAAGCCAGAGAACTGGTTAACATGTTGGAAAAGATTGCCGGGCCCATT GGCATGCGCATAAGCCCCCCAGCCTGGGTTGAGCTGAAGGATGACCGAATAGAGACCTATATCAGGACCA TTCAGTCCTTACTGGGAGTTGAGGGGAAGATACAAATGGTCGTTTGCATCATCATGGGCACACGTGATGA TCTCTATGGAGCCATCAAGAAGCTGTGCTGCGTGCAGTCCCCAGTGCCCTCACAGGTCATCAATGTCCGA ACCATTGGTCAGCCCACCAGGCTTCGGAGCGTGGCTCAGAAAATTTTACTTCAGATGAACTGTAAACTGG GTGGTGAGCTCTGGGGAGTGGATATTCCGCTGAAACAACTAATGGTGATTGGAATGGATGTGTACCATGA CCCCAGCAGAGGCATGCGCTCTGTGGTCGGCTTCGTGGCCAGCATAAATCTCACACTCACCAAATGGTAC TCGAGGGTGGTGTTCCAGATGCCACATCAGGAGATTGTGGACAGCCTGAAGCTCTGCCTGGTGGGTTCCT TGAAAAAGTATTATGAGGTGAACCATTGTCTCCCAGAGAAAATTGTGGTGTACCGAGATGGAGTGTCTGA TGGCCAGCTAAAGACAGTTGCCAACTACGAGATCCCTCAGCTGCAGAAGTGTTTTGAAGCCTTTGATAAC TACCACCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGCACCAATCTGTACCTTGCTGCTCCTG ATCACTTCGTAACCCCCTCCCCCGGGACTGTGGTTGATCATACCATAACCAGCTGTGAGTGGGTGGATTT CTACCTTCTTGCCCATCATGTGCGACAGGGCTGTGGCATACCTACACACTACATCTGTGTTCTGAACACT GCAAATCTGAGCCCTGATCACATGCAGAGGTTGACTTTCAAACTATGCCACATGTACTGGAATTGGCCTG GTACCATCCGAGTTCCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCCGGACAGATTTTGCA TCATGAGCCAGCCATCCAGCTGTGTGGGAACCTGTTCTTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223877 representing NM_021308
Red=Cloning site Green=Tags(s) MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSPPLQPVQKDSVGLV SMFRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSR MLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKE LLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSILYLP VKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVL QQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRY NNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEIL LLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHK IEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPI GMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVR TIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWY SRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDN YHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNT ANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021308 |
| ORF Size | 2913 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021308.2 |
| RefSeq Size | 4913 bp |
| RefSeq ORF | 2916 bp |
| Locus ID | 57746 |
| UniProt ID | Q8CDG1 |
| Gene Summary | Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:14736746, PubMed:17446352, PubMed:18381894, PubMed:18922463, PubMed:26669262). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (PubMed:22020280, PubMed:23706823, PubMed:26669262). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (PubMed:28633017). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (PubMed:18381894, PubMed:18922463, PubMed:26669262). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:19114715). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (PubMed:16261612). Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins (PubMed:28903391).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222906 | Piwil2 (untagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2), (10ug) |
CNY 12,540.00 |
|
| MR223877 | Piwil2 (Myc-DDK-tagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2) |
CNY 7,112.00 |
|
| MR223877L3 | Lenti ORF clone of Piwil2 (Myc-DDK-tagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2) |
CNY 9,030.00 |
|
| MR223877L4 | Lenti ORF clone of Piwil2 (mGFP-tagged) - Mouse piwi-like homolog 2 (Drosophila) (Piwil2) |
CNY 9,030.00 |
