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Adamts5 (NM_011782) Mouse Tagged ORF Clone
CAT＃: MG224554
- TrueORF®
Adamts5 (tGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif 5 (aggrecanase-2) (Adamts5), (10ug)
ORF Plasmid: DDK
"NM_011782" in other vectors (4)
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CNY 11,816.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9530092O11Rik; ADAM-; ADAM-TS5; ADAMTS1; ADAMTS11; ADMP-2; AI481094; ASMP-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224554 representing NM_011782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCGAGTGGGCGCCCTTGTTGCTGCTACTGCTGCTGCTGAGCGCGTCCTGCCTGTCCCTGGCCG CTGACAGCCCCGCCGCGGCACCTGCCCAGGATAAAACCAGGCAGCCTCAGGCTGCAGCAGCGGCCGCCGA GCCGGACCAGCCGCAGGGGGAGGAAACACGGGAGCGAGGCCATTTACAACCCTTGGCCGGGCAGCGCAGG AGCGGCGGGCTGGTCCAGAATATAGACCAACTCTACTCTGGCGGTGGCAAAGTGGGCTACCTTGTCTACG CGGGCGGCCGGAGGTTCCTGCTGGACCTGGAGAGAGATGACACAGTGGGTGCTGCTGGTAGCATCGTTAC TGCAGGAGGAGGGCTGAGCGCATCCTCTGGCCACCGGGGTCACTGTTTCTACAGAGGCACCGTGGACGGC AGCCCTCGATCCCTAGCTGTCTTTGACCTCTGCGGGGGTCTCGATGGCTTCTTTGCAGTCAAGCATGCGC GCTACACTCTAAAGCCACTCCTGCGTGGGTCCTGGGCAGAGTATGAACGAATTTATGGGGATGGATCTTC CCGCATCCTGCATGTCTACAACCGCGAGGGCTTTAGCTTCGAGGCCCTGCCGCCACGCGCCAGTTGCGAG ACTCCTGCATCCCCATCTGGGCCCCAAGAGAGCCCCTCGGTGCACAGTAGATCTAGGAGACGCTCAGCGC TGGCCCCGCAGCTGCTGGACCACTCAGCTTTCTCGCCATCTGGGAACGCGGGACCTCAGACTTGGTGGAG GCGTAGGCGCCGTTCCATCTCCAGGGCCCGCCAGGTGGAGCTCCTCTTGGTGGCTGACTCGTCCATGGCC AGGATGTATGGGCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAACAGGCTGTACAGTC ATGCAAGCATTGAGAACCACATCCGCCTGGCGGTGGTGAAGGTGGTTGTGCTGACGGACAAGGACACGAG TCTGGAGGTGAGCAAGAATGCGGCCACGACCCTCAAGAACTTTTGCAAATGGCAGCACCAACATAACCAG CTAGGGGATGATCACGAAGAGCACTACGATGCAGCCATCCTGTTCACCCGAGAGGATTTATGTGGGCATC ATTCATGTGACACCCTGGGAATGGCAGACGTTGGGACCATATGTTCTCCGGAGCGCAGCTGTGCAGTGAT TGAAGATGATGGCCTCCATGCAGCCTTCACTGTGGCTCATGAAATTGGGCATCTACTTGGCCTTTCTCAT GACGATTCCAAATTCTGTGAAGAGAACTTCGGTACTACAGAAGACAAGCGTTTAATGTCTTCAATCCTTA CCAGCATCGATGCATCCAAGCCCTGGTCCAAATGCACGTCAGCCACCATCACAGAATTCCTGGATGATGG TCATGGTAATTGTTTGCTAGACCTACCACGGAAGCAGATTTTGGGTCCCGAGGAACTCCCAGGACAGACC TACGATGCCACCCAGCAGTGCAACTTGACATTTGGGCCTGAGTACTCGGTGTGCCCTGGCATGGATGTCT GTGCGCGGCTGTGGTGTGCTGTGGTGCGCCAAGGCCAAATGGTGTGTCTGACCAAGAAGCTGCCGGCTGT GGAGGGCACTCCCTGTGGGAAGGGAAGAGTCTGCCTTCAAGGCAAATGTGTGGACAAAACTAAGAAAAAA TATTACTCGACATCAAGCCATGGAAATTGGGGGTCCTGGGGCCCCTGGGGTCAGTGTTCTCGCTCATGCG GGGGAGGAGTGCAGTTTGCCTACCGCCATTGTAATAACCCTGCACCTCGAAACAGTGGCCGCTACTGCAC AGGGAAGAGGGCCATATACCGTTCCTGCAGTGTTACACCCTGCCCACCCAATGGTAAATCTTTTCGCCAT GAGCAGTGTGAAGCCAAAAATGGCTATCAGTCTGATGCAAAAGGAGTCAAAACATTTGTAGAATGGGTTC CCAAATATGCAGGTGTCCTGCCGGCAGATGTGTGCAAGCTTACCTGCAGAGCTAAGGGCACAGGCTACTA TGTGGTCTTTTCTCCAAAGGTTACGGATGGGACTGAATGCAGGCCGTACAGCAACTCTGTGTGTGTCCGA GGACGGTGTGTGAGAACTGGATGTGACGGCATTATTGGCTCAAAGCTACAATATGACAAGTGTGGAGTGT GCGGAGGGGATAACTCCAGTTGTACAAAGATTATCGGAACCTTCAATAAAAAAAGCAAGGGTTATACTGA CGTTGTGAGGATCCCTGAAGGAGCAACCCACATAAAAGTCCGACAGTTCAAAGCCAAAGACCAGACTAGA TTCACTGCCTACTTAGCCCTGAAGAAGAAAACTGGCGAGTACCTTATCAATGGCAAGTACATGATTTCCA CTTCAGAGACCATCATCGACATCAATGGTACCGTCATGAACTACAGTGGATGGAGCCACAGAGATGATTT TTTACATGGGATGGGCTATTCAGCCACAAAAGAAATCCTGATCGTGCAGATCCTTGCCACAGACCCAACT AAAGCGCTAGACGTCCGTTACAGCTTTTTTGTTCCCAAGAAGACCACTCAAAAAGTAAACTCTGTCATCA GCCATGGCAGCAACAAGGTGGGACCACACTCTACACAGCTGCAGTGGGTGACAGGTCCATGGCTGGCCTG CTCCAGGACCTGTGACACAGGCTGGCACACTAGGACCGTGCAGTGCCAGGATGGAAACAGGAAATTAGCT AAAGGATGCCTTCTCTCTCAGAGGCCTTCTGCATTTAAGCAATGTCTGCTGAAGAAATGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224554 representing NM_011782
Red=Cloning site Green=Tags(s) MRLEWAPLLLLLLLLSASCLSLAADSPAAAPAQDKTRQPQAAAAAAEPDQPQGEETRERGHLQPLAGQRR SGGLVQNIDQLYSGGGKVGYLVYAGGRRFLLDLERDDTVGAAGSIVTAGGGLSASSGHRGHCFYRGTVDG SPRSLAVFDLCGGLDGFFAVKHARYTLKPLLRGSWAEYERIYGDGSSRILHVYNREGFSFEALPPRASCE TPASPSGPQESPSVHSRSRRRSALAPQLLDHSAFSPSGNAGPQTWWRRRRRSISRARQVELLLVADSSMA RMYGRGLQHYLLTLASIANRLYSHASIENHIRLAVVKVVVLTDKDTSLEVSKNAATTLKNFCKWQHQHNQ LGDDHEEHYDAAILFTREDLCGHHSCDTLGMADVGTICSPERSCAVIEDDGLHAAFTVAHEIGHLLGLSH DDSKFCEENFGTTEDKRLMSSILTSIDASKPWSKCTSATITEFLDDGHGNCLLDLPRKQILGPEELPGQT YDATQQCNLTFGPEYSVCPGMDVCARLWCAVVRQGQMVCLTKKLPAVEGTPCGKGRVCLQGKCVDKTKKK YYSTSSHGNWGSWGPWGQCSRSCGGGVQFAYRHCNNPAPRNSGRYCTGKRAIYRSCSVTPCPPNGKSFRH EQCEAKNGYQSDAKGVKTFVEWVPKYAGVLPADVCKLTCRAKGTGYYVVFSPKVTDGTECRPYSNSVCVR GRCVRTGCDGIIGSKLQYDKCGVCGGDNSSCTKIIGTFNKKSKGYTDVVRIPEGATHIKVRQFKAKDQTR FTAYLALKKKTGEYLINGKYMISTSETIIDINGTVMNYSGWSHRDDFLHGMGYSATKEILIVQILATDPT KALDVRYSFFVPKKTTQKVNSVISHGSNKVGPHSTQLQWVTGPWLACSRTCDTGWHTRTVQCQDGNRKLA KGCLLSQRPSAFKQCLLKKC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011782 |
| ORF Size | 2790 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011782.2, NP_035912.2 |
| RefSeq Size | 8104 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 23794 |
| UniProt ID | Q9R001 |
| Gene Summary | This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. Mice lacking the encoded protein are protected from surgery-induced osteoarthritis and antigen-induced arthritis. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222688 | Adamts5 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5), (10ug) |
CNY 10,224.00 |
|
| MR224554 | Adamts5 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5) |
CNY 10,216.00 |
|
| MR224554L3 | Lenti ORF clone of Adamts5 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5) |
CNY 11,210.00 |
|
| MR224554L4 | Lenti ORF clone of Adamts5 (mGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) (Adamts5) |
CNY 11,210.00 |
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