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Flt4 (NM_008029) Mouse Tagged ORF Clone
CAT＃: MG225313
- TrueORF®
Flt4 (tGFP-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4), (10ug)
ORF Plasmid: DDK
"NM_008029" in other vectors (6)
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CNY 15,592.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI323512; Chy; Flt-4; VEGFR-3; VEGFR3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225313 representing NM_008029
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGGGCGCTGCGCTGAACCTGCGCCTGTGGCTCTGCCTCGGACTCCTCCAAGGCCTGGCAAATG GTTACTCCATGACCCCTCCAACCCTGAACATCACAGAGGATTCATATGTGATTGACACCGGGGACAGCCT ATCCATATCCTGCAGGGGACAGCACCCCCTCGAGTGGACCTGGCCAGGGGCCCAGGAGGTACTGACCACA GGTGGGAAGGACAGTGAAGACACACGGGTTGTGCATGACTGTGAAGGCACAGAAGCTAGGCCCTACTGCA AGGTGCTACTGCTGGCCCAGACTCACGCCAACAACACGGGCAGCTACCACTGCTACTACAAGTACATCAA GGCCCGGATTGAGGGCACCACAGCTGCCAGCACCTATGTGTTTGTAAGAGACTTTAAACACCCTTTCATC AACAAACCTGACACGCTCCTGGTCAACAGGAAGGACTCGATGTGGGTGCCCTGCTTGGTGTCCATTCCCG GCCTCAACATCACACTGCGCTCGCAAAGCTCAGCGCTGCACCCCGACGGGCAGGAGGTGCTGTGGGATGA CCGCCGGGGCATGCGGGTGCCCACTCAACTGTTGCGCGATGCCCTGTACCTGCAGTGCGAGACCACCTGG GGTGACCAGAACTTCCTTTCCAATCTCTTCGTCGTGCACATCACAGGCAATGAGCTCTATGACATCCAGC TGTACCCCAAGAAGTCAATGGAGCTGTTGGTTGGAGAGAAGCTGGTTTTGAACTGTACAGTGTGGGCTGA GTTCGACTCAGGTGTCACCTTCGACTGGGATTATCCAGGGAAGCAGGCAGAGCGGGCTAAGTGGGTACCT GAGCGGCGTTCCCAGCAGACCCACACAGAACTCTCCAGCATCCTGACCATCCACAATGTCAGCCAGAATG ACCTGGGCCCCTATGTGTGTGAGGCCAACAATGGGATTCAGCGGTTCCGGGAAAGCACAGAGGTCATTGT GCACGAAAAGCCCTTCATCAGTGTCGAGTGGCTCAAAGGACCTGTCCTGGAGGCCACAGCCGGTGACGAG CTGGTGAAGCTACCCGTGAAGCTGGCAGCTTATCCCCCACCGGAGTTCCAATGGTACAAGGACAGAAAGG CAGTGACTGGGCGCCACAATCCCCATGCTCTGGTGCTCAAAGAGGTGACCGAGGCCAGCGCAGGGGTCTA CACTCTCGCCCTGTGGAACTCTGCAGCTGGTCTGAGGCAAAACATCAGTCTGGAGCTGGTGGTGAATGTG CCTCCTCACATCCACGAAAAGGAAGCCTCTTCACCCAGCATCTACTCCCGCCACAGTCGCCAGACCCTCA CCTGCACCGCCTATGGAGTACCCCAACCCCTCAGTGTCCAGTGGCACTGGAGGCCCTGGACACCCTGCAA GACGTTTGCCCAGCGCAGCCTCCGGAGGCGGCAGCAGCGGGATGGCATGCCACAGTGCCGAGACTGGAAG GAGGTGACCACTCAGGATGCTGTGAACCCCATCGAGAGTCTGGACAGCTGGACGGAGTTTGTGGAGGGGA AGAATAAGACGGTGAGCAAGCTGGTGATCCAGGATGCCAATGTGTCAGCCATGTACAAGTGTGTGGTCGT CAACAAAGTGGGCCAGGATGAGAGACTCATCTACTTCTATGTGACCACCATCCCGGACGGTTTCAGTATC GAGTCGGAGCCTTCTGAGGATCCCTTAGAAGGCCAGTCCGTGCGCCTCAGCTGCCGGGCGGACAACTACA CGTACGAACATCTGCGCTGGTACCGGCTCAACCTCTCCACACTGCACGATGCTCAAGGGAACCCCCTATT GCTGGACTGCAAAAACGTGCACCTGTTTGCCACGCCCCTAGAGGCCAACCTAGAGGAGGCAGAGCCCGGG GCCCGCCACGCCACCCTCAGTTTGAATATCCCCCGAGTGGCGCCCGAGGACGAGGGTGACTACGTGTGTG AAGTGCAGGATAGGCGCAGCCAGGACAAGCACTGCCACAAGAAGTACCTGTCCGTGCAGGCCCTGGAAGC TCCTCGGCTCACGCAGAACTTGACCGACCTCCTGGTGAACGTGAGTGACTCCCTGGAGATGCGATGCCCG GTGGCTGGAGCGCATGTGCCCAGTATTGTGTGGTACAAAGATGAAAGGCTCCTGGAGAAAGAGTCGGGAA TCGACCTGGCAGACTCCAATCAGAGGCTGAGCATCCAGCGCGTGCGCGAGGAGGACGCAGGTCGTTATCT GTGCAGCGTGTGCAATGCCAAGGGCTGCGTAAACTCCTCTGCCAGCGTGGCAGTGGAAGGCTCTGAAGAT AAAGGCAGCATGGAGATTGTGATACTCATTGGCACTGGCGTCATCGCAGTTTTCTTCTGGGTCCTCCTCC TGCTCATCTTCTGTAACATGAAAAGGCCTGCCCATGCAGACATCAAGACGGGCTACCTGTCCATCATCAT GGACCCCGGGGAGGTGCCTTTGGAGGAGCAGTGTGAATACCTGTCCTATGACGCCAGCCAGTGGGAGTTC CCCAGGGAAAGGTTGCACCTCGGGAGAGTCCTAGGCCACGGGGCTTTTGGGAAGGTGGTGGAAGCCTCAG CTTTTGGCATCAATAAAGGCAGCAGCTGTGACACCGTGGCTGTGAAGATGCTGAAAGAGGGCGCTACTGC CAGCGAGCACCGTGCCCTGATGTCGGAGCTCAAGATCCTAATTCACATCGGCAACCATCTCAACGTGGTC AACCTCCTAGGGGCGTGCACCAAGCCCAACGGCCCTCTCATGGTGATCGTGGAGTTTTGCAAATACGGCA ACCTCTCCAACTTCTTGCGTGTCAAGCGGGACACGTTCAACCCCTACGCGGAGAAGTCTCCGGAGCAACG CAGGCGCTTCCGCGCCATGGTAGAAGGCGCCAAAGCTGATAGGAGGAGACCTGGAAGCAGCGACAGGGCC CTGTTCACGCGGTTCCTGATGGGCAAAGGAAGTGCACGGCGAGCCCCACTTGTCCAAGAAGCTGAGGACC TATGGCTGAGCCCACTGACCATGGAAGACCTTGTATGCTACAGCTTCCAAGTTGCCCGGGGAATGGAGTT CCTGGCTTCCCGCAAGTGCATTCACAGAGACCTGGCTGCTCGGAACATCCTACTGTCAGAAAGTGACATA GTGAAGATCTGCGACTTTGGCCTCGCTCGGGACATCTACAAAGACCCCGACTATGTCCGAAAGGGCAGTG CCCGACTTCCTCTGAAATGGATGGCCCCCGAGAGCATCTTTGATAAGGTGTACACCACGCAGAGTGATGT GTGGTCCTTCGGCGTGCTGCTGTGGGAGATCTTCTCATTGGGGGCCTCTCCATACCCTGGGGTACAGATC AATGAGGAGTTCTGCCAGCGGCTGAAGGATGGCACTCGAATGAGAGCCCCGGAACTGGCCACTCCTGCCA TACGCCACATCATGCAGAGTTGCTGGTCTGGAGACCCTAAAGCAAGACCTGCTTTCTCTGACCTAGTGGA GATCCTGGGGGACCTGCTTCAGGGCGGAGGCTGGCAGGAGGAGGAAGAGGAGCGCATGGCCCTGCACAGC TCTCAGAGCTCCGAGGAGGATGGCTTCATGCAGGCATCCACCACAGCTCTACATATCACCGAAGCAGACG CTGATGATAGTCCACCCAGCATGCATTGCCACAGCCTGGCAGCCAGATATTACAACTGTGTGTCCTTTCC TGGGCGCCTGGCCAGAGGCACTAAGACTCCAGGCTCTTCCAGGATGAAGACATTTGAAGAATTGCCCATG ACCCCTACAACCTACAAAGCCTCCATGGATAACCAGACAGACAGCGGGATGGTGCTGGCCTCAGAAGAGT TTGAGGAGCTAGAAAGCAGGCATAGACCAGAAGGCAGCTTCAGCTGTAAAGGTCCTGGCCAGCACATGGA TATTCCCAGAGGACACCCTGACCCCCAGGGGAGGCGGCGACGGCCCACTCAAGGGGCACAAGGAGGCAAG GTGTTTTATAACAACGAGTATGGGGAGGTCTCCCAGCCATGTACAGAAGGTGACTGCTGCCCGTCTGCTG GCTCCACCTTCTTCGCAGACAGCAGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225313 representing NM_008029
Red=Cloning site Green=Tags(s) MQPGAALNLRLWLCLGLLQGLANGYSMTPPTLNITEDSYVIDTGDSLSISCRGQHPLEWTWPGAQEVLTT GGKDSEDTRVVHDCEGTEARPYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTAASTYVFVRDFKHPFI NKPDTLLVNRKDSMWVPCLVSIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPTQLLRDALYLQCETTW GDQNFLSNLFVVHITGNELYDIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFDWDYPGKQAERAKWVP ERRSQQTHTELSSILTIHNVSQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISVEWLKGPVLEATAGDE LVKLPVKLAAYPPPEFQWYKDRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSAAGLRQNISLELVVNV PPHIHEKEASSPSIYSRHSRQTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLRRRQQRDGMPQCRDWK EVTTQDAVNPIESLDSWTEFVEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDERLIYFYVTTIPDGFSI ESEPSEDPLEGQSVRLSCRADNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHLFATPLEANLEEAEPG ARHATLSLNIPRVAPEDEGDYVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMRCP VAGAHVPSIVWYKDERLLEKESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSED KGSMEIVILIGTGVIAVFFWVLLLLIFCNMKRPAHADIKTGYLSIIMDPGEVPLEEQCEYLSYDASQWEF PRERLHLGRVLGHGAFGKVVEASAFGINKGSSCDTVAVKMLKEGATASEHRALMSELKILIHIGNHLNVV NLLGACTKPNGPLMVIVEFCKYGNLSNFLRVKRDTFNPYAEKSPEQRRRFRAMVEGAKADRRRPGSSDRA LFTRFLMGKGSARRAPLVQEAEDLWLSPLTMEDLVCYSFQVARGMEFLASRKCIHRDLAARNILLSESDI VKICDFGLARDIYKDPDYVRKGSARLPLKWMAPESIFDKVYTTQSDVWSFGVLLWEIFSLGASPYPGVQI NEEFCQRLKDGTRMRAPELATPAIRHIMQSCWSGDPKARPAFSDLVEILGDLLQGGGWQEEEEERMALHS SQSSEEDGFMQASTTALHITEADADDSPPSMHCHSLAARYYNCVSFPGRLARGTKTPGSSRMKTFEELPM TPTTYKASMDNQTDSGMVLASEEFEELESRHRPEGSFSCKGPGQHMDIPRGHPDPQGRRRRPTQGAQGGK VFYNNEYGEVSQPCTEGDCCPSAGSTFFADSSY TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008029 |
| ORF Size | 4089 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008029.3, NP_032055.1 |
| RefSeq Size | 5853 bp |
| RefSeq ORF | 4092 bp |
| Locus ID | 14257 |
| UniProt ID | P35917 |
| Gene Summary | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224341 | Flt4 (untagged) - Mouse FMS-like tyrosine kinase 4 (Flt4), (10ug) |
CNY 17,480.00 |
|
| MR225313 | Flt4 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4) |
CNY 13,992.00 |
|
| MR225313L1 | Lenti ORF clone of Flt4 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4) |
CNY 15,490.00 |
|
| MR225313L2 | Lenti ORF clone of Flt4 (mGFP-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4) |
CNY 16,392.00 |
|
| MR225313L3 | Lenti ORF clone of Flt4 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4) |
CNY 15,490.00 |
|
| MR225313L4 | Lenti ORF clone of Flt4 (mGFP-tagged) - Mouse FMS-like tyrosine kinase 4 (Flt4) |
CNY 15,490.00 |
