United States
Germany
Japan
United Kingdom
China
Pkd2l1 (NM_181422) Mouse Tagged ORF Clone
CAT＃: MG225337
- TrueORF®
Pkd2l1 (tGFP-tagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1), (10ug)
ORF Plasmid: DDK
"NM_181422" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B830002B15; BC046386; PCL; PKD2L; Pkdl; TRPP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225337 representing NM_181422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAGTATGGAAAGCCCCAAGAATCAGGAGCTACAAACCCTGGGGAACAGAGCCTGGGACAATCCTG CCTACAGCGACCCTCCTTCCCCGAACAGGACGCTGAGGATCTGCACTGTCTCCAGTGTGGCTCTCCCTGA GACTCAACCCAAAAAGCCAGAAGTCAGATGCCAGGAGAAGACACAGAGAACCCTGGTGTCCAGCTGCTGT CTCCATATCTGTCGGAGCATCAGAGGACTGTGGGGGACAACGCTGACTGAGAACACAGCCGAGAACAGGG AGCTTTATGTCAAGACCACCCTAAGGGAGCTTGTGGTATACATAGTGTTCCTCGTGGACGTCTGTCTGTT GACCTACGGAATGACAAGTTCTAGTGCCTATTACTACACCAAAGTGATGTCTGAATTGTTCCTACACACC CCATCCGACTCTGGAGTCTCCTTCCAAACCATCAGCAGCATGTCAGACTTCTGGGATTTTGCTCAGGGCC CACTCCTGGACAGTTTGTACTGGACAAAGTGGTACAACAACCAGAGCCTGGGGCGTGGCTCCCACTCCTT CATCTACTATGAGAACCTGCTCCTGGGAGCCCCAAGGTTGCGGCAGCTGCGCGTGCGCAATGACTCCTGT GTGGTTCATGAAGACTTCCGGGAGGACATTTTGAACTGTTATGATGTGTACTCGCCGGACAAAGAAGATC AGCTCCCCTTTGGACCTCAGAACGGCACAGCGTGGACATACCATTCCCAGAATGAGCTGGGTGGCTCCTC CCACTGGGGCAGGCTCACAAGCTACAGCGGGGGTGGCTACTACTTGGATCTTCCAGGATCCCGACAAGCC AGTGCAGAGGCCCTCCAAGGACTCCAGGAGGGACTGTGGCTGGACAGGGGCACTCGGGTGGTCTTTATCG ACTTCTCCGTCTACAATGCCAACATCAATCTTTTCTGTATTCTGAGACTGGTGGTAGAGTTTCCAGCCAC AGGAGGGACCATCCCATCCTGGCAGATCCGCACAGTTAAGCTGATCCGCTATGTGAATAACTGGGACTTC TTCATTGTGGGCTGTGAAGTTGTCTTCTGTGTCTTCATCTTCTATTATGTGGTGGAGGAAATCCTGGAAA TCCACCTGCATCGGCTTCGCTACCTCAGCAGCGTCTGGAACATTCTGGACCTGGTGGTCATCTTGCTCTC CATCGTGGCTGTGGGTTTCCACATATTCCGAACCCTGGAAGTGAACCGACTGATGGGAAAGCTTCTGCAA CAGCCAGACACGTATGCAGACTTTGAGTTCCTGGCCTTCTGGCAGACTCAGTACAATAACATGAACGCGG TCAACCTTTTCTTTGCTTGGATCAAGATATTCAAGTATATCAGCTTCAACAAGACCATGACACAGCTCTC CTCCACCCTGGCTCGATGTGCCAAGGACATCCTGGGCTTCGCCATCATGTTCTTCATTGTCTTCTTCGCT TACGCCCAGCTTGGTTACCTGCTTTTTGGGACCCAAGTGGAAAACTTTAGCACTTTCGTCAAGTGCATTT TCACTCAGTTCCGGATAATCCTTGGGGATTTTGACTACAATGCCATCGACAATGCCAACAGAATCCTGGG CCCTGTGTACTTTGTCACCTATGTCTTCTTCGTCTTCTTCGTGCTCCTGAACATGTTCCTGGCCATCATC AACGACACATACTCCGAGGTCAAGGAGGAGCTGGCTGGCCAGAAGGATCAGTTGCAGCTTTCTGACTTCC TGAAACAGAGCTACAACAAGACCCTACTAAGGCTGCGCCTGAGGAAAGAGCGGGTTTCTGATGTGCAGAA GGTCCTGAAGGGTGGGGAACCAGAGATCCAGTTTGAAGATTTCACCAGCACCTTGAGGGAACTGGGGCAC GAGGAGCACGAGATCACCGCTGCCTTCACCAGGTTTGATCAGGATGGGGACCACATACTGGATGAGGAGG AGCAGGAACAGATGCGGCAGGGACTGGAAGAGGAGAGGGTGACCCTCAATGCTGAGATTGAGAACCTAGG CCGGTCTGTTGGACACAGCCCCCCAGGCGAATTGGGCGCGGAGGCTGCCAGAGGACAAAGCTGGGTTTCT GGAGAAGAATTCGACATGCTCACAAGGAGAGTTCTGCAGCTGCAGTGTGTTCTGGAAGGAGTTGTGTCCC AGATTGATGCTGTAGGCTCAAAGCTGAAGATGCTGGAGAGGAAAGGGGAGCTGGCTCCCTCCCCAGGAAT GGGGGAACCAGCTGTTTGGGAGAACCCGTATAATCCGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225337 representing NM_181422
Red=Cloning site Green=Tags(s) MNSMESPKNQELQTLGNRAWDNPAYSDPPSPNRTLRICTVSSVALPETQPKKPEVRCQEKTQRTLVSSCC LHICRSIRGLWGTTLTENTAENRELYVKTTLRELVVYIVFLVDVCLLTYGMTSSSAYYYTKVMSELFLHT PSDSGVSFQTISSMSDFWDFAQGPLLDSLYWTKWYNNQSLGRGSHSFIYYENLLLGAPRLRQLRVRNDSC VVHEDFREDILNCYDVYSPDKEDQLPFGPQNGTAWTYHSQNELGGSSHWGRLTSYSGGGYYLDLPGSRQA SAEALQGLQEGLWLDRGTRVVFIDFSVYNANINLFCILRLVVEFPATGGTIPSWQIRTVKLIRYVNNWDF FIVGCEVVFCVFIFYYVVEEILEIHLHRLRYLSSVWNILDLVVILLSIVAVGFHIFRTLEVNRLMGKLLQ QPDTYADFEFLAFWQTQYNNMNAVNLFFAWIKIFKYISFNKTMTQLSSTLARCAKDILGFAIMFFIVFFA YAQLGYLLFGTQVENFSTFVKCIFTQFRIILGDFDYNAIDNANRILGPVYFVTYVFFVFFVLLNMFLAII NDTYSEVKEELAGQKDQLQLSDFLKQSYNKTLLRLRLRKERVSDVQKVLKGGEPEIQFEDFTSTLRELGH EEHEITAAFTRFDQDGDHILDEEEQEQMRQGLEEERVTLNAEIENLGRSVGHSPPGELGAEAARGQSWVS GEEFDMLTRRVLQLQCVLEGVVSQIDAVGSKLKMLERKGELAPSPGMGEPAVWENPYNPS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181422 |
| ORF Size | 2280 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181422.3, NP_852087.2 |
| RefSeq Size | 3321 bp |
| RefSeq ORF | 2283 bp |
| Locus ID | 329064 |
| UniProt ID | A2A259 |
| Gene Summary | Pore-forming subunit of a heteromeric, non-selective cation channel that is permeable to Ca(2+) (PubMed:16891422, PubMed:15548533, PubMed:19464260, PubMed:20538909, PubMed:21185261, PubMed:22420714, PubMed:25820328, PubMed:28904867, PubMed:29567962). Pore-forming subunit of a calcium-permeant ion channel formed by PKD1L2 and PKD1L1 in primary cilia, where it controls cilium calcium concentration, but does not affect cytoplasmic calcium concentration (PubMed:24336288, PubMed:24336289). The channel formed by PKD1L2 and PKD1L1 in primary cilia regulates sonic hedgehog/SHH signaling and GLI2 transcription (PubMed:24336288). Pore-forming subunit of a channel formed by PKD1L2 and PKD1L3 that contributes to sour taste perception in gustatory cells (PubMed:16891422, PubMed:16929298, PubMed:20406802, PubMed:21098668, PubMed:21625513). The heteromeric channel formed by PKD1L2 and PKD1L3 is activated by low pH, but opens only when the extracellular pH rises again (PubMed:18535624, PubMed:19464260, PubMed:20538909, PubMed:20406802, PubMed:22420714, PubMed:28904867, PubMed:29567962). May play a role in the perception of carbonation taste (PubMed:19833970). May play a role in the sensory perception of water, via a mechanism that activates the channel in response to dilution of salivary bicarbonate and changes in salivary pH (PubMed:28553944).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221408 | Pkd2l1 (untagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1), (10ug) |
CNY 9,790.00 |
|
| MR225337 | Pkd2l1 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1) |
CNY 5,568.00 |
|
| MR225337L3 | Lenti ORF clone of Pkd2l1 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1) |
CNY 7,700.00 |
|
| MR225337L4 | Lenti ORF clone of Pkd2l1 (mGFP-tagged) - Mouse polycystic kidney disease 2-like 1 (Pkd2l1) |
CNY 7,700.00 |
