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Ireb2 (NM_022655) Mouse Tagged ORF Clone
CAT＃: MG226153
- TrueORF®
Ireb2 (tGFP-tagged) - Mouse iron responsive element binding protein 2 (Ireb2), (10ug)
ORF Plasmid: DDK
"NM_022655" in other vectors (4)
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CNY 12,176.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D9Ertd85e; Irp2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226153 representing NM_022655
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCCCAAGTGCAGGATACACCTTTGAGTACCTTATTGAAACATTAAATGGCAATTCACAGAAGA AGTTCTTCAATGTACCTAAACTTGGCGGCACCAAGTATGATATTCTGCCTTACTCAATACGGGTCCTATT GGAAGCTGCTGTACGAAATTGTGATGGCTTTTTAATGAAAAAGGAAGATGTTATGAACATTTTGGACTGG AAAACCAAACAGAGCAATGTTGAAGTGCCCTTTTTCCCTGCCCGTGTTGTTCTTCAAGATTTCACTGGAA TACCGGCAATGGTGGATTTTGCTGCTATGAGGGAGGCAGTGAAAACTCTTGGAGGTGATCCTAAGAAAGT CCACCCTGCCTGTCCGACAGATCTCACAGTGGACCACTCTTTACAGATTGACTTCAGTAAATGTGCAATA CAGAATGCACCAAATCCTGGAGGTGGTGACCTACAGAAAGCAGGAAAGCTCTCTCCACTTAAAGTGCAGT CTAAGAAGCTTCCATGTCGAGGCCAGACTACCTGCCGGGGATCGTGTGATTCTGGAGAGCTAAGCCGGAA CTCAGGAACATTTTCTTCACAGATTGAGAATACACCTGTCTTGTGTCCTTTTCATTTGCAACCAGTGCCT GAACCTGAGACAGTGTTAAAAAATCAAGAAGTAGAATTTGGCAGAAATCGAGAGAGGCTTCAGTTTTTCA AGTGGAGCTCAGGAGCTTTTAAGAATGTGGCAGTCATCCCGCCTGGAACTGGAATGGCTCATCAAGTGAA CTTAGAATATTTATCAAGAGTAGTTTTTGAAGAAACGGACCTGCTCTTCCCAGACAGTGTAGTTGGCACA GATTCTCATATAACCATGGTGAACGGATTGGGGATTCTTGGGTGGGGAGTTGGTGGCATTGAGACAGAGG CAGTTATGCTTGGCCTGCCAGTTACTCTTACTTTACCAGAGGTGGTTGGATGTGAGCTAACTGGGTCATC CAATGCTTTTGTTACATCCATAGATATTGTCCTTGGCATTACAAAGCACCTCAGGCAAGTAGGCGTGGCT GGAAAGTTTGTTGAGTTCTTTGGAAGTGGAGTTTCACAATTATCTATTGTCGATAGAACTACCATAGCAA ACATGTGTCCAGAATATGGTGCTATCCTCAGCTTTTTCCCTGTTGACAATGTGACACTACGACATTTAGA ACATACAGGTTTTGACAAAACCAAACTTGAGTCAATGGAAAAATACCTTAAAGCTGTGAAATTGTTTCGA AATGATGAGAATTCTTCAGAACCTGAATATTCTCAGGTGATACAGATTAATCTGAATTCAATAGTTGCAT CTGTCAGTGGTCCAAAAAGGCCTCAGGATAGGGTTGCTGTAACAGATATGAAAAGTGATTTTCAGGCTTG CTTAAATGAAAAGGTTGGATTTAAAGGCTTCCAAGTTGCAGCAGAAAAACAAAGTGACACTGTCTCTGTT CGTTATGATGGAAGTGAGTATAAGCTGTCCCATGGATCCGTGGTCATTGCTGCAGTTATCAGTTGTACCA ATAATTGCAATCCATCTGTGATGCTTGCTGCAGGTCTTCTGGCTAAAAAGGCTGTTGAAATTGGTCTACG AGTTAAACCTTATATACGAACAAGTTTGTCTCCAGGCAGTGGGATGGTTACACATTACCTCAGTTCAAGT GGAGTGCTACCCTATCTTAGCAAGCTAGGGTTTGACATAGTTGGCTATGGATGTTCAACATGTGTAGGAA ATACAGCACCTTTATCAGAAGCAGTTTTGAATGCAGTAAAACAGGGTGATTTGGTTACCTGTGGAGTTTT ATCTGGAAACAAACATTTCGAAGGCCGTCTCTGTGATTGTGACCGTGCCAATTATCTCGCCTCTCCGCCC TTAGTGGTGGCTTATGCCATAGCAGGCACAGTGAATATAGACTTCCAGACAGAGCCTTTAGGTACTGACT CTACGGGCAAGGAAATTTACCTGCATGACATTTGGCCTAGTCGAGAAGAAGTTCATCAGATGGAGGAAGA ACATGTTATATTGTCAATGTTTAAAACACTAAAAGAGAAAGTAGAGATGGGAAATAAACGGTGGAATTCG TTAGAAGCTCCAGACTCCGTGCTGTTTCCATGGGATGTGAAGTCTACTTACATCAGATGCCCTTCATTTT TTGATAAGCTTACCAAAGAGCCAGCTGCATCCCAGCCTATTGAGAATGCCCATGTCCTGTTGTATTTGGG AGACTCTGTCACTACAGATCATATATCACCCGCTGGAAGTATTGCTAGGAGTAGTGCTGCTGCTAAGTAT TTGACAAACAGAGGCCTTACTCCCCGAGAGTTCAACTCTTATGGAGCTCGAAGAGGTAATGATGCTGTGA TGACAAGAGGCACATTTGCAAACATCAAGCTTTTTAATAAGTTTATTGGGAAGCCAGCTCCCAAAACAAT TCATTTTCCATCGGGACAAACGCTCGATGTATTTGAAGCTGCAGAACTGTACCAGAAAGAAGGTATCCCA CTGATAATTTTAGCAGGAAAAAAATATGGTTCAGGAAATTCAAGAGATTGGGCTGCCAAGGGACCCTATT TGCTGGGTGTAAAAGCTGTTTTGGCTGAGAGCTATGAAAAAATACACAAAGATCATTTGATTGGAATTGG TATAGCACCACTTGAATTCCTCCCAGGAGAAAATGCAGATTCCTTGGGCCTCTCTGGCAGAGAAGTATTT TCTTTATCATTTCCTGAAGAACTCTTTCCTGGAATTACATTAAATATAAAGACAAGCACTGGAAAAGAGT TCAGCGTGATTGCATCATTTGCAAATGACGTGGAGATAACTTTGTACAAACATGGAGGCTTATTAAACTT TGTGGCTCGAAAATTCTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226153 representing NM_022655
Red=Cloning site Green=Tags(s) MDSPSAGYTFEYLIETLNGNSQKKFFNVPKLGGTKYDILPYSIRVLLEAAVRNCDGFLMKKEDVMNILDW KTKQSNVEVPFFPARVVLQDFTGIPAMVDFAAMREAVKTLGGDPKKVHPACPTDLTVDHSLQIDFSKCAI QNAPNPGGGDLQKAGKLSPLKVQSKKLPCRGQTTCRGSCDSGELSRNSGTFSSQIENTPVLCPFHLQPVP EPETVLKNQEVEFGRNRERLQFFKWSSGAFKNVAVIPPGTGMAHQVNLEYLSRVVFEETDLLFPDSVVGT DSHITMVNGLGILGWGVGGIETEAVMLGLPVTLTLPEVVGCELTGSSNAFVTSIDIVLGITKHLRQVGVA GKFVEFFGSGVSQLSIVDRTTIANMCPEYGAILSFFPVDNVTLRHLEHTGFDKTKLESMEKYLKAVKLFR NDENSSEPEYSQVIQINLNSIVASVSGPKRPQDRVAVTDMKSDFQACLNEKVGFKGFQVAAEKQSDTVSV RYDGSEYKLSHGSVVIAAVISCTNNCNPSVMLAAGLLAKKAVEIGLRVKPYIRTSLSPGSGMVTHYLSSS GVLPYLSKLGFDIVGYGCSTCVGNTAPLSEAVLNAVKQGDLVTCGVLSGNKHFEGRLCDCDRANYLASPP LVVAYAIAGTVNIDFQTEPLGTDSTGKEIYLHDIWPSREEVHQMEEEHVILSMFKTLKEKVEMGNKRWNS LEAPDSVLFPWDVKSTYIRCPSFFDKLTKEPAASQPIENAHVLLYLGDSVTTDHISPAGSIARSSAAAKY LTNRGLTPREFNSYGARRGNDAVMTRGTFANIKLFNKFIGKPAPKTIHFPSGQTLDVFEAAELYQKEGIP LIILAGKKYGSGNSRDWAAKGPYLLGVKAVLAESYEKIHKDHLIGIGIAPLEFLPGENADSLGLSGREVF SLSFPEELFPGITLNIKTSTGKEFSVIASFANDVEITLYKHGGLLNFVARKFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022655 |
| ORF Size | 2889 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022655.2 |
| RefSeq Size | 5808 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 64602 |
| UniProt ID | Q811J3 |
| Gene Summary | RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3' UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222876 | Ireb2 (untagged) - Mouse iron responsive element binding protein 2 (Ireb2), (10ug) |
CNY 12,350.00 |
|
| MR226153 | Ireb2 (Myc-DDK-tagged) - Mouse iron responsive element binding protein 2 (Ireb2) |
CNY 10,576.00 |
|
| MR226153L3 | Lenti ORF clone of Ireb2 (Myc-DDK-tagged) - Mouse iron responsive element binding protein 2 (Ireb2) |
CNY 11,500.00 |
|
| MR226153L4 | Lenti ORF clone of Ireb2 (mGFP-tagged) - Mouse iron responsive element binding protein 2 (Ireb2) |
CNY 11,500.00 |
