Trib3 (NM_175093) Mouse Tagged ORF Clone
CAT#: MG226343
- TrueORF®
Trib3 (tGFP-tagged) - Mouse tribbles homolog 3 (Drosophila) (Trib3), (10ug)
ORF Plasmid: DDK
"NM_175093" in other vectors (3)
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CNY 3,900.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Ifld2; Nipk; SINK; SKIP3; TRB-3; Trb3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG226343 representing NM_175093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGCTACACCTCTGGCTGCTTCTGCTGATGTTTCCTGCAGGAAGAAACCGTTGGAGTTTGATGACA ATATTGATGCCAAGTGTCCAGTCCTAAAACGAGTGAGAGATGAGCCTGAGCCCGGACCACTCCCCAGCCT GCTGCCCCCCAGCCCACCTCCCGCCTCAGACTTGTCACCTGCTGTGGCCCCTGCAACTCGACTGGGGCCT TATATCCTTTTGGAACGAGAGCAAGGCAGCTGCAGCTATCGAGCCCTGCACTGCCCCACAGGCACAGAGT ACACCTGCAAGGTGTACCCTGCCAGCGAGGCCCAGGCGGTGCTGGCACCTTATGCCCGGCTGCCTACCCA CCAGCATGTGGCCCGTCCCACAGAGGTCCTGCTGGGCTCTCGGCTCCTTTACATCTTTTTCACGAAGACC CATGGGGACTTGCACAGCCTGGTGCGCAGCCGCCGCGGTATCCCAGAGTCCGAGGCTGCCGGGCTCTTCC GGCAGATGGCTAGTGCCGTGGCACACTGCCACAAGCACGGGCTTGTCTTGCGCGACCTCAAGCTGCGTCG CTTTGTCTTCAGCAACTGTGAGAGGACGAAGCTGGTGCTGGAGAACCTGGAAGATGCCTGCGTGATGACT GGATCAGATGACTCTCTGTGGGACAAGCATGCGTGCCCTGCCTACGTGGGACCAGAGATACTCAGCTCCC GGCCATCCTACTCTGGCAAAGCGGCTGATGTCTGGAGCCTGGGTGTGGCGCTCTTCACCATGCTGGCTGG CAGATACCCATTCCACGACTCTGAGCCAGTCCTGCTCTTTGGCAAGATCCGTAGAGGGACCTTTGCCCTG CCTGAGGGCCTATCAGCCCCAGCCCGCTGTCTGATCCGCTGTCTCCTCCGCAAGGAACCTTCAGAGCGAC TTGTGGCCCTGGGAATCCTCTTGCATCCCTGGTTGAGAGAGGATCACGGCCGAGTCTCTCCTCCACAGTC TGACCGAAGGGAGATGGACCAGGTGGTCCCAGATGGGCCACAGCTGGAGGAGGCTGAGGAAGGGGAGGTG GGGCTGTACGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226343 representing NM_175093
Red=Cloning site Green=Tags(s) MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGP YILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKT HGDLHSLVRSRRGIPESEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMT GSDDSLWDKHACPAYVGPEILSSRPSYSGKAADVWSLGVALFTMLAGRYPFHDSEPVLLFGKIRRGTFAL PEGLSAPARCLIRCLLRKEPSERLVALGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEV GLYG TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175093 |
ORF Size | 1062 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175093.2, NP_780302.2 |
RefSeq Size | 2051 bp |
RefSeq ORF | 1065 bp |
Locus ID | 228775 |
UniProt ID | Q8K4K2 |
Gene Summary | Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). Can inhibit APOBEC3A editing of nuclear DNA.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A Drosophila model of insulin resistance associated with the human Trib3 Q/R polymorphism
,Fischer, Z;Das, R;Shipman, A;Fan, JY;Pence, L;Bouyain, S;Dobens, LL;,
Dis Model Mech
,PubMed ID 29025897
[TRIB3]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR226343 | Trib3 (Myc-DDK-tagged) - Mouse tribbles homolog 3 (Drosophila) (Trib3) |
CNY 3,520.00 |
|
MR226343L3 | Lenti ORF clone of Trib3 (Myc-DDK-tagged) - Mouse tribbles homolog 3 (Drosophila) (Trib3) |
CNY 5,420.00 |
|
MR226343L4 | Lenti ORF clone of Trib3 (mGFP-tagged) - Mouse tribbles homolog 3 (Drosophila) (Trib3) |
CNY 5,420.00 |