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P2ry1 (NM_008772) Mouse Tagged ORF Clone
CAT＃: MG226766
- TrueORF®
P2ry1 (tGFP-tagged) - Mouse purinergic receptor P2Y G-protein coupled 1 (P2ry1), (10ug)
ORF Plasmid: DDK
"NM_008772" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | P2Y1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226766 representing NM_008772
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAGGTGCCTTGGTCGGTTGTCCCCAACGGGACGGATGCTGCCTTTCTGGCTGGCCTGGGCTCGC TTTGGGGAAACAGTACTGTCGCCTCAACTGCAGCAGTTTCCTCTTCATTCCAATGTGCCCTGACCAAGAC CGGTTTCCAGTTCTACTACCTGCCGGCCGTCTACATTTTAGTGTTCATCATAGGCTTCCTAGGCAACAGC GTGGCTATCTGGATGTTCGTTTTCCACATGAAGCCTTGGAGCGGCATCTCCGTGTACATGTTCAATTTGG CTCTGGCTGACTTTTTATACGTGCTCACCCTACCAGCCCTCATCTTCTACTACTTCAACAAGACTGACTG GATCTTCGGGGATGCTATGTGCAAGCTGCAGAGATTCATCTTCCACGTAAACCTCTACGGTAGCATCTTG TTCCTCACCTGCATCAGCGCACACAGGTACAGTGGCGTGGTGTACCCTCTCAAGTCTCTGGGCAGGCTCA AGAAGAAGAATGCCATTTATGTTAGCGTGCTGGTGTGGCTCATTGTGGTGGTGGCCATCTCCCCTATTCT CTTCTACTCTGGCACTGGGACTCGGAAAAACAAAACTGTCACCTGCTATGACACCACGTCCAATGATTAC CTGCGAAGTTATTTCATCTACAGTATGTGCACGACTGTGGCCATGTTCTGCATCCCTTTGGTGCTGATCT TGGGCTGTTATGGATTAATTGTTAAAGCTTTGATTTACAATGACCTGGACAACTCTCCGCTCCGGAGGAA ATCCATTTACCTGGTGATAATTGTCCTGACGGTGTTTGCTGTGTCTTATATCCCTTTCCATGTGATGAAA ACGATGAATTTGCGAGCACGGTTGGATTTCCAGACCCCAGAAATGTGTGATTTCAACGACAGGGTTTATG CCACTTATCAGGTAACAAGAGGTCTAGCAAGTCTCAACAGCTGTGTGGACCCCATTCTTTATTTCTTGGC TGGAGATACATTCAGAAGGAGACTGTCCCGAGCCACCAGGAAAGCTTCCAGGAGGAGTGAGGCCAATTTA CAATCCAAGAGTGAAGAAATGACTCTCAATATTTTATCTGAGTTCAAGCAGAATGGAGACACGAGTTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226766 representing NM_008772
Red=Cloning site Green=Tags(s) MTEVPWSVVPNGTDAAFLAGLGSLWGNSTVASTAAVSSSFQCALTKTGFQFYYLPAVYILVFIIGFLGNS VAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSIL FLTCISAHRYSGVVYPLKSLGRLKKKNAIYVSVLVWLIVVVAISPILFYSGTGTRKNKTVTCYDTTSNDY LRSYFIYSMCTTVAMFCIPLVLILGCYGLIVKALIYNDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMK TMNLRARLDFQTPEMCDFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANL QSKSEEMTLNILSEFKQNGDTSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008772 |
| ORF Size | 1119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008772.4, NP_032798.1 |
| RefSeq Size | 3886 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 18441 |
| UniProt ID | P49650 |
| Gene Summary | Receptor for extracellular adenine nucleotides such as ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209137 | P2ry1 (untagged) - Mouse purinergic receptor P2Y, G-protein coupled 1 (P2ry1), (10ug) |
CNY 3,990.00 |
|
| MR226766 | P2ry1 (Myc-DDK-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 1 (P2ry1) |
CNY 3,330.00 |
|
| MR226766L3 | Lenti ORF clone of P2ry1 (Myc-DDK-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 1 (P2ry1) |
CNY 5,230.00 |
|
| MR226766L4 | Lenti ORF clone of P2ry1 (mGFP-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 1 (P2ry1) |
CNY 5,230.00 |
