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Scn10a (NM_009134) Mouse Tagged ORF Clone
CAT＃: MG226793
- TrueORF®
Scn10a (tGFP-tagged) - Mouse sodium channel voltage-gated type X alpha (Scn10a), (10ug)
ORF Plasmid: DDK
"NM_009134" in other vectors (2)
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CNY 23,660.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Nav1.8; PN3; SNS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226793 representing NM_009134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCTTTGGGTCCGTGGGAACTACCAACTTCAGACGGTTCACTCCAGAGTCGCTGGCAGAGA TCGAGAAGCAGATCGCTGCCCACCGCGCCGCCAAGAAGGGCAGACCTAAGCAAAGAGGACAGAAGGACAA GAGTGAGAAGCCCAGGCCTCAGTTGGACTTGAAGGCCTGTAACCAGCTGCCCAGGTTCTATGGCGAGCTC CCAGCAGAGCTGGTCGGGGAGCCCCTGGAGGACCTGGATCCTTTCTACAGCACACACCGGACATTCATAG TGTTGGATAAAAGCAGGACCATTTCCAGATTCAGTGCCACTTGGGCTCTGTGGCTCTTCAGTCCCTTCAA CCTGATCAGAAGAACAGCCATCAAAGTGTCCGTCCACTCCTGGTTCTCCATATTTATCACTGTCACTATT TTGGTCAACTGTGTGTGCATGACCCGAACTGATCTTCCAGAGAAACTCGAGTATGCCTTCACTGTTGTTT ACACCTTCGAGGCTCTGATAAAGATACTGGCAAGAGGGTTTTGTCTAAATGAATTCACTTATCTTCGAGA TCCCTGGAACTGGCTGGACTTCAGTGTCATTACCCTGGCGTATGTGGGTGCAGCGATAGACCTCCGAGGA ATCTCAGGCCTGCGGACATTCCGAGTTCTCAGGGCCCTGAAGACTGTTTCTGTGATCCCAGGACTGAAGG TCATCGTGGGAGCCCTGATCCACTCAGTGAGGAAGCTGGCCGACGTGACCATCCTCACAGTCTTCTGCCT GAGTGTCTTTGCCTTGGTGGGCCTGCAGCTCTTCAAGGGGAACCTCAAGAATAAATGCATTAAGAACGGC ACAGATCCGCACAAGGCTGACAATCTCTCATCTGAAATGGCAGGAGACATCTTCATCAAGCCCGGTACTA CGGATCCCTTGTTGTGTGGCAATGGATCTGATGCTGGCCACTGCCCTAATGATTATGTCTGCCGGAAAAC TTCTGACAACCCGGATTTTAACTACACCAGCTTTGATTCCTTCGCGTGGGCGTTCCTCTCACTGTTCCGT CTCATGACGCAGGACTCCTGGGAACGGCTGTACCAGCAGACACTCCGGGCTTCCGGGAAAATGTACATGG TCTTTTTTGTGCTGGTCATCTTCCTTGGATCATTCTACCTGGTCAATTTGATCTTGGCTGTGGTCACCAT GGCATATGAGGAACAGAGCCAGGCAACAATTGCAGAAATCGAAGCCAAGGAGAAGAAGTTCAAGGAAGCC CTCGAGGTGCTGCAGAAAGAACAGGAGGTGCTGGCAGCGCTGGGCATTGACACAACCTCGCTCTATTCCC ACAACGGATCACCCTTAGCCCCCAAAAACGCCAATGAGAGAAGACCCAGGGTGAAATCAAGGATGTCAGA AGGCTCGACAGATGACAACAGATCACTACAATCCGACCCTTACAACCAGCGCAGGATGTCTTTCCTAGGC CTTTCTTCTGGAAGACGCAGGGCTAGCCACAGCAGTGTGTTCCACTTCCGAGCACCCAGCCAAGACGTCT CATTTCCTGATGGGATCTTGGATGACGGGGTCTTTCATGGAGATCAGGAAAGCCGTCGAAGTTCCATATT GCTGGGCAGGGGTGCCGGGCAGGCAGGTCCTCTCCCCAGGAGTCCACTGCCTCAGTCCCCCAACCCTGGC CCTAGACGTGGAGAAGAGGGACAGCGTGGAGTGCCCACTGGTGAGCTTGCCACTGGAGCGCCTGAAGGCC CGGCACTCGATGCTGCAGGACAGAAGAACTTCCTGTCTGCAGACTACTTGAATGAACCTTTCCGAGCACA GAGGGCAATGAGTGTTGTCAGTATTATGACTTCTGTCATTGAGGAGCTGGAAGAGTCTAAGCTGAAGTGC CCACCCTGCTTGATCAGCTTAGCCCAGAAGTACCTGATATGGGAGTGCTGCCCCAAGTGGAAGAAATTCA AGATGGTGCTCTTCGAGCTGGTGACTGACCCCTTCGCAGAGCTCACCATCACCCTGTGCATTGTGGTGAA TACCGTCTTCATGGCCATGGAACACTACCCCATGACTGATGCCTTCGATGCCATGCTCCAAGCCGGCAAC ATTGTCTTTACTGTGTTTTTTACAATGGAGATGGCCTTCAAGATCATTGCCTTCGACCCCTACTACTACT TCCAGAAGAAGTGGAACATCTTCGACTGTGTCATCGTCACCGTGAGCCTGCTGGAGCTGAGCACATCCAA GAAGGGCAGCTTGTCTGTGCTCCGCACCTTCCGCTTGCTTCGGGTCTTCAAGCTGGCCAAGTCCTGGCCC ACCCTGAACATGCTCATCAAGATCATCGGGAACTCTGTGGGGGCCCTGGGCAACCTGACCTTCATCCTGG CCATCATCGTCTTTATCTTCGCCCTGGTGGGAAAGCAGCTCCTCTCAGAGAACTATGGGTGCCGCAGGGA TGGCATCTCCGTGTGGAATGGTGAGAGGCTGCGCTGGCACATGTGTGACTTCTTCCATTCCTTCCTCGTC GTCTTCCGGATCCTCTGCGGGGAGTGGATCGAGAACATGTGGGTCTGCATGGAGGTCAGCCAGGACTACA TCTGCCTCACCCTCTTCTTGACAGTGATGGTGCTAGGCAACCTGGTGGTGCTCAACCTATTCATCGCTTT ACTGCTGAACTCCTTCAGTGCGGACAACCTCACAGCCCCAGAGGATGACGGGGAGGTGAACAACTTGCAG GTAGCACTGGCCCGGATTCAGGTATTTGGCCATCGGGCCAGTCGGGCCATTACCAGTTACATCAGAAGCC ACTGCCGGCTCCGCTGGCCCAAGGTGGAGACCCAGCTGGGGATGAAACCCCCACTCACCAGCTGCAAAGC TGAGAACCACATTGCTACTGATGCTGTCAATGCTGCAGTGGGGAACCTGGCAAAGCCAGCTCTTGGTGGC CCCAAGGAGAACCACGGGGACTTCATCACTGATCCTAACGTGTGGGTCTCTGTGCCCATTGCTGAGGGGG AGTCCGACCTTGATGAGCTCGAGGAAGATGTGGAGCATGCTTCTCAGAGCTCCTGGCAGGAAGAGAGCCC CAAAGGGCAGGAGCTGCTGCAGCAAGTCCAAAAGTGTGAAGATCACCAGGCAGCCCGAAGCCCACCCTCC GGGATGTCCTCTGAGGACCTGGCTCCATACCTGGGGGAGAGATGGCAGAGGGAGGAGAGCCCTCGGGTCC CTGCCGAGGGAGTGGATGACACAAGCTCCTCCGAGGGCAGCACGGTGGACTGCCCGGACCCAGAGGAGAT CCTGAGGAAGATCCCTGAGCTGGCGGAGGAGCTGGACGAGCCCGATGACTGTTTCCCAGAAGGCTGCACT CGCCGCTGTCCCTGCTGCAAAGTGAACACCAGTAAGTTTCCTTGGGCCACGGGCTGGCAGGTGCGCAAAA CCTGTTACCGCATTGTGGAGCACAGCTGGTTTGAGAGTTTCATTATCTTCATGATCCTGCTCAGCAGTGG AGCGCTGGCCTTTGAGGATAACTACCTGGAAGAAAAGCCCCGAGTGAAGTCTGTGCTGGAGTACACTGAC AGAGTGTTCACCTTCATCTTTGTATTCGAGATGTTGCTCAAGTGGGTAGCTTATGGCTTCAAAAAATATT TCACCAATGCCTGGTGCTGGCTGGACTTCCTCATCGTGAATATCTCCCTCACAAGCCTCATAGCCAAGAT CCTCGAGTATTCAGACGTGGCGTCCATCAAAGCCCTTCGGACTCTCCGTGCCCTCCGGCCGCTGCGGGCT CTGTCTCGATTCGAAGGCATGAGGGTAGTGGTGGATGCCTTGGTGGGCGCCATTCCCTCCATCATGAACG TCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGTGTGAACCTCTTCGCCGGGAAATT TTCGAGATGTGTCGACACCAGAAGCAACCCATTTTCCGTCGTGAATTCGACATTCGTGACTAACAAGTCT GACTGTTACAATCAAAACAATACTGGCCACTTCTTCTGGGTTAACGTCAAAGTCAACTTCGACAACGTTG CTATGGGCTACCTCGCGCTTCTCCAGGTGGCAACCTTCAAAGGCTGGATGGACATTATGTATGCAGCTGT CGATTCTCGAGATATCAACAGTCAGCCCAATTGGGAGGAGAGCCTGTACATGTACCTATACTTCGTCGTC TTCATCATTTTCGGTGGCTTCTTCACGCTGAATCTCTTTGTCGGGGTCATCATTGACAACTTCAATCAAC AGAAAAAAAAGCTAGGGGGCCAGGACATCTTCATGACAGAGGAGCAGAAGAAGTACTACAATGCCATGAA GAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTTTGAATAAGTACCAGGGCTTCGTGTTT GACATTGTGACCAGGCAAGCATTTGACATCATCATCATGGCTCTCATCTGCCTCAACATGATCACCATGA TGGTGGAGACCGACAATCAGAGCGAGGAGAAGACGAAGGTCCTGGGCAGAATCAACCAGTTCTTCGTGGC CGTCTTCACGGGCGAGTGTGTGATGAAGATGTTCGCCCTTCGGCAGTATTACTTCACCAACGGCTGGAAT GTGTTCGACTTCATTGTGGTGATTCTGTCCATTTCTAGTCTGTTGTTTTCTGCGATCCTTAGCTCACTAG AAAGTTACTTCTCCCCCACGCTCTTACGCGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCTGAT TCGAGCAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAACATC GGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAGCTTCGCTAATGTCATAG ATGAGGCTGGCATCGACGACATGTTCAACTTCAAGACCTTTGGCAACAGCATGCTGTGCCTTTTCCAGAT CACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGGACCCCCCTACTGCGACCCC AACCGGCCCAACAGCAATGGCTCCAAGGGGAATTGTGGAAGCCCAGCGGTGGGCATCCTCTTCTTCACCA CCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCAGTGATTCTGGAGAACTTCAATGT GGCCACAGAAGAGAGCACGGAGCCCCTGAGCGAGGACGACTTTGACATGTTCTATGAGACCTGGGAGAAG TTTGACCCGGAGGCCACCCAGTTCATTGCCTTTTCTGCCCTCTCAGACTTTGCAGACACACTCTCTGGCC CTCTTAGAATCCCAAAACCTAATCAGAATATATTAATCCAGATGGACCTGCCGTTGGTCCCCGGAGATAA GATCCACTGTTTGGACATCCTCTTTGCCTTCACAAAGAATGTCTTGGGAGAATCTGGGGAGTTGGATTCT CTGAAGACTAATATGGAAGAGAAGTTTATGGCAACTAATCTTTCCAAAGCATCCTATGAACCAATAGCAA CCACCCTCCGGTGCAAGCAGGAAGACATCTCAGCCACCATTATTCAAAAGGCCTATCGGAACTACATGTT GCAACGCTCCTTGATGCTCTCCAACCCCCTGCATGTGCCCAGGGCTGAGGAGGATGGCGTGTCACTCCCC AGGGAAGGCTATGTTACATTCATGGCAAATGACAACGGTGGGCTCCCAGACAAATCGGAAACTGCTTCTG CTACGTCTTTCCCACCATCCTATGACAGCGTCACCAGGGGCCTGAGTGACAGGGCCAACATTAGCACATC TAGCTCAATGCAAAATGAAGATGAAGTCACTGCTAAGGAAGGGAAGAGCCCTGGACCTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226793 representing NM_009134
Red=Cloning site Green=Tags(s) MEFPFGSVGTTNFRRFTPESLAEIEKQIAAHRAAKKGRPKQRGQKDKSEKPRPQLDLKACNQLPRFYGEL PAELVGEPLEDLDPFYSTHRTFIVLDKSRTISRFSATWALWLFSPFNLIRRTAIKVSVHSWFSIFITVTI LVNCVCMTRTDLPEKLEYAFTVVYTFEALIKILARGFCLNEFTYLRDPWNWLDFSVITLAYVGAAIDLRG ISGLRTFRVLRALKTVSVIPGLKVIVGALIHSVRKLADVTILTVFCLSVFALVGLQLFKGNLKNKCIKNG TDPHKADNLSSEMAGDIFIKPGTTDPLLCGNGSDAGHCPNDYVCRKTSDNPDFNYTSFDSFAWAFLSLFR LMTQDSWERLYQQTLRASGKMYMVFFVLVIFLGSFYLVNLILAVVTMAYEEQSQATIAEIEAKEKKFKEA LEVLQKEQEVLAALGIDTTSLYSHNGSPLAPKNANERRPRVKSRMSEGSTDDNRSLQSDPYNQRRMSFLG LSSGRRRASHSSVFHFRAPSQDVSFPDGILDDGVFHGDQESRRSSILLGRGAGQAGPLPRSPLPQSPNPG PRRGEEGQRGVPTGELATGAPEGPALDAAGQKNFLSADYLNEPFRAQRAMSVVSIMTSVIEELEESKLKC PPCLISLAQKYLIWECCPKWKKFKMVLFELVTDPFAELTITLCIVVNTVFMAMEHYPMTDAFDAMLQAGN IVFTVFFTMEMAFKIIAFDPYYYFQKKWNIFDCVIVTVSLLELSTSKKGSLSVLRTFRLLRVFKLAKSWP TLNMLIKIIGNSVGALGNLTFILAIIVFIFALVGKQLLSENYGCRRDGISVWNGERLRWHMCDFFHSFLV VFRILCGEWIENMWVCMEVSQDYICLTLFLTVMVLGNLVVLNLFIALLLNSFSADNLTAPEDDGEVNNLQ VALARIQVFGHRASRAITSYIRSHCRLRWPKVETQLGMKPPLTSCKAENHIATDAVNAAVGNLAKPALGG PKENHGDFITDPNVWVSVPIAEGESDLDELEEDVEHASQSSWQEESPKGQELLQQVQKCEDHQAARSPPS GMSSEDLAPYLGERWQREESPRVPAEGVDDTSSSEGSTVDCPDPEEILRKIPELAEELDEPDDCFPEGCT RRCPCCKVNTSKFPWATGWQVRKTCYRIVEHSWFESFIIFMILLSSGALAFEDNYLEEKPRVKSVLEYTD RVFTFIFVFEMLLKWVAYGFKKYFTNAWCWLDFLIVNISLTSLIAKILEYSDVASIKALRTLRALRPLRA LSRFEGMRVVVDALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFSRCVDTRSNPFSVVNSTFVTNKS DCYNQNNTGHFFWVNVKVNFDNVAMGYLALLQVATFKGWMDIMYAAVDSRDINSQPNWEESLYMYLYFVV FIIFGGFFTLNLFVGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFVF DIVTRQAFDIIIMALICLNMITMMVETDNQSEEKTKVLGRINQFFVAVFTGECVMKMFALRQYYFTNGWN VFDFIVVILSISSLLFSAILSSLESYFSPTLLRVIRLARIGRILRLIRAAKGIRTLLFALMMSLPALFNI GLLLFLVMFIYSIFGMASFANVIDEAGIDDMFNFKTFGNSMLCLFQITTSAGWDGLLSPILNTGPPYCDP NRPNSNGSKGNCGSPAVGILFFTTYIIISFLIVVNMYIAVILENFNVATEESTEPLSEDDFDMFYETWEK FDPEATQFIAFSALSDFADTLSGPLRIPKPNQNILIQMDLPLVPGDKIHCLDILFAFTKNVLGESGELDS LKTNMEEKFMATNLSKASYEPIATTLRCKQEDISATIIQKAYRNYMLQRSLMLSNPLHVPRAEEDGVSLP REGYVTFMANDNGGLPDKSETASATSFPPSYDSVTRGLSDRANISTSSSMQNEDEVTAKEGKSPGPQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009134 |
| ORF Size | 5871 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009134.3, NP_033160.2 |
| RefSeq Size | 6368 bp |
| RefSeq ORF | 5874 bp |
| Locus ID | 20264 |
| UniProt ID | Q6QIY3 |
| Gene Summary | Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.[UniProtKB/Swiss-Prot Function] |
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