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Mecp2 (NM_001081979) Mouse Tagged ORF Clone
CAT＃: MG226839
- TrueORF®
Mecp2 (tGFP-tagged) - Mouse methyl CpG binding protein 2 (Mecp2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001081979" in other vectors (4)
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CNY 4,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1500041B07Rik; D630021H01Rik; Mbd5; WBP10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226839 representing NM_001081979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCTGCCGCCACCGCCGCCGCCGCCGCCGCGCCGAGCGGAGGAGGAGGAGGAGGCGAGGAGG AGAGACTGGAGGAAAAGTCAGAAGACCAGGATCTCCAGGGCCTCAGAGACAAGCCACTGAAGTTTAAGAA GGCGAAGAAAGACAAGAAGGAGGACAAAGAAGGCAAGCATGAGCCACTACAACCTTCAGCCCACCATTCT GCAGAGCCAGCAGAGGCAGGCAAAGCAGAAACATCAGAAAGCTCAGGCTCTGCCCCAGCAGTGCCAGAAG CCTCGGCTTCCCCCAAACAGCGGCGCTCCATTATCCGTGACCGGGGACCTATGTATGATGACCCCACCTT GCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAAAGTATGATGTATAT TTGATCAATCCCCAGGGAAAAGCTTTTCGCTCTAAAGTAGAATTGATTGCATACTTTGAAAAGGTGGGAG ACACCTCCTTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCAGGAGAGAGCA GAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGGGGTCGGGGACGCCCCAAAGGG AGCGGCACTGGGAGACCAAAGGCAGCAGCATCAGAAGGTGTTCAGGTGAAAAGGGTCCTGGAGAAGAGCC CTGGGAAACTTGTTGTCAAGATGCCTTTCCAAGCATCGCCTGGGGGTAAGGGTGAGGGAGGTGGGGCTAC CACATCTGCCCAGGTCATGGTGATCAAACGCCCTGGCAGAAAGCGAAAAGCTGAAGCTGACCCCCAGGCC ATTCCTAAGAAACGGGGTAGAAAGCCTGGGAGTGTGGTGGCAGCTGCTGCAGCTGAGGCCAAAAAGAAAG CCGTGAAGGAGTCTTCCATACGGTCTGTGCATGAGACTGTGCTCCCCATCAAGAAGCGCAAGACCCGGGA GACGGTCAGCATCGAGGTCAAGGAAGTGGTGAAGCCCCTGCTGGTGTCCACCCTTGGTGAGAAAAGCGGG AAGGGACTGAAGACCTGCAAGAGCCCTGGGCGTAAAAGCAAGGAGAGCAGCCCCAAGGGGCGCAGCAGCA GTGCCTCCTCCCCACCTAAGAAGGAGCACCATCATCACCACCATCACTCAGAGTCCACAAAGGCCCCCAT GCCACTGCTCCCATCCCCACCCCCACCTGAGCCTGAGAGCTCTGAGGACCCCATCAGCCCCCCTGAGCCT CAGGACTTGAGCAGCAGCATCTGCAAAGAAGAGAAGATGCCCCGAGGAGGCTCACTGGAAAGCGATGGCT GCCCCAAGGAGCCAGCTAAGACTCAGCCTATGGTCGCCACCACTACCACAGTTGCAGAAAAGTACAAACA CCGAGGGGAGGGAGAGCGCAAAGACATTGTTTCATCTTCCATGCCAAGGCCAAACAGAGAGGAGCCTGTG GACAGCCGGACGCCCGTGACCGAGAGAGTTAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226839 representing NM_001081979
Red=Cloning site Green=Tags(s) MAAAAATAAAAAAPSGGGGGGEEERLEEKSEDQDLQGLRDKPLKFKKAKKDKKEDKEGKHEPLQPSAHHS AEPAEAGKAETSESSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVY LINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKG SGTGRPKAAASEGVQVKRVLEKSPGKLVVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQA IPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVHETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSG KGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESTKAPMPLLPSPPPPEPESSEDPISPPEP QDLSSSICKEEKMPRGGSLESDGCPKEPAKTQPMVATTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPV DSRTPVTERVS TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081979 |
| ORF Size | 1503 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081979.2 |
| RefSeq Size | 10152 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 17257 |
| UniProt ID | Q9Z2D6 |
| Gene Summary | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216963 | Mecp2 (untagged) - Mouse methyl CpG binding protein 2 (Mecp2), transcript variant 1, (10ug) |
CNY 3,736.00 |
|
| MR226839 | Mecp2 (Myc-DDK-tagged) - Mouse methyl CpG binding protein 2 (Mecp2), transcript variant 1 |
CNY 3,728.00 |
|
| MR226839L3 | Lenti ORF clone of Mecp2 (Myc-DDK-tagged) - Mouse methyl CpG binding protein 2 (Mecp2), transcript variant 1 |
CNY 6,270.00 |
|
| MR226839L4 | Lenti ORF clone of Mecp2 (mGFP-tagged) - Mouse methyl CpG binding protein 2 (Mecp2), transcript variant 1 |
CNY 6,128.00 |
