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Nefh (NM_010904) Mouse Tagged ORF Clone
CAT＃: MG227134
- TrueORF®
Nefh (tGFP-tagged) - Mouse neurofilament heavy polypeptide (Nefh), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_010904" in other vectors (2)
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CNY 12,984.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA0845; NF-H; NF200; Nfh |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227134 representing NM_010904, codon optimized.
Due to the complexity of NM_010904, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTCCTTCGGATCAGCAGACGCGCTTCTCGGCGCCCCTTTTGCTCCTCTTCATGGCGGCGGTTCTC TTCATTACAGCCTGAGCCGCAAAGCAGGGCCAGGCGGGACCCGGAGCGCTGCTGGCTCCAGTAGCGGTTT CCACTCATGGGCCCGCACCAGCGTCAGTTCTGTCAGTGCTAGTCCAAGCAGGTTTCGGGGAGCAGCCTCT TCTACGGACTCTCTGGATACCCTTAGCAACGGGCCCGAGGGATGTGTCGTTGCAGCCGTCGCTGCGCGCA GCGAGAAGGAGCAGCTGCAGGCTCTGAATGATCGCTTTGCTGGATACATTGATAAAGTTCGGCAACTGGA AGCTCATAATAGATCTCTCGAAGGTGAGGCAGCCGCCCTCAGGCAGCAGCAGGCCGGACGAGCCGCTATG GGAGAACTCTACGAAAGAGAGGTCCGGGAAATGAGAGGGGCCGTGCTCAGACTGGGCGCTGCTCGAGGGC AATTGAGGCTCGAGCAGGAACACTTGCTCGAGGATATCGCCCACGTCCGGCAGAGACTGGACGAGGAGGC TAGACAGCGCGAAGAGGCCGAAGCTGCCGCCAGAGCCTTGGCACGCTTCGCCCAAGAGGCTGAGGCTGCT CGCGTTGAACTGCAGAAGAAAGCCCAGGCACTGCAGGAAGAATGTGGATACCTGCGCCGGCACCATCAGG AAGAAGTCGGGGAGTTGCTGGGACAGATTCAAGGCTGCGGCGCCGCCCAGGCACAAGCCCAGGCCGAAGC CCGGGACGCTCTGAAATGTGATGTCACGAGCGCACTCCGCGAGATACGAGCCCAGCTGGAGGGGCACGCC GTGCAGTCAACACTCCAGTCAGAGGAATGGTTCAGAGTCCGGCTGGATCGACTGTCCGAGGCCGCAAAGG TGAATACAGACGCCATGCGCTCTGCACAGGAAGAGATAACAGAGTACAGACGGCAGCTGCAGGCTAGGAC TACCGAGCTCGAAGCACTCAAGAGCACCAAGGAGAGTTTGGAGAGACAACGGAGCGAGCTGGAGGATAGG CACCAGGCTGACATTGCTTCATACCAAGACGCGATCCAACAGTTGGATTCCGAACTGCGCAATACCAAAT GGGAGATGGCCGCTCAGCTCAGAGAATACCAAGACCTGTTGAATGTCAAGATGGCTCTGGATATTGAGAT TGCTGCTTACCGCAAACTGCTGGAAGGCGAAGAGTGCCGGATAGGCTTTGGCCCCTCACCATTTTCTCTG ACAGAAGGACTTCCCAAGATCCCCAGCATCAGTACTCATATCAAAGTTAAAAGTGAGGAAATGATCAAGG TGGTAGAGAAGAGCGAAAAAGAAACGGTCATAGTGGAGGGTCAAACTGAAGAAATCAGGGTGACCGAGGG CGTGACCGAGGAAGAAGATAAAGAAGCGCAAGGGCAAGAGGGGGAGGAAGCCGAGGAAGGTGAAGAAAAG GAGGAAGAAGAAGGGGCTGCTGCCACATCCCCGCCAGCAGAAGAGGCCGCCAGCCCAGAGAAAGAGACTA AGAGCCGGGTGAAGGAGGAAGCCAAATCACCTGGCGAGGCCAAATCTCCCGGAGAGGCCAAAAGCCCAGC TGAAGCAAAGTCCCCAGGGGAGGCAAAGTCCCCAGGTGAGGCCAAGTCTCCCGGAGAGGCCAAGTCTCCA GCTGAGCCAAAGTCACCAGCGGAGCCAAAAAGTCCTGCTGAAGCAAAGAGCCCCGCAGAGCCTAAATCTC CCGCAACCGTCAAAAGCCCAGGCGAGGCTAAATCCCCTAGTGAGGCCAAATCTCCCGCTGAAGCCAAGAG CCCCGCCGAGGCTAAAAGCCCCGCAGAAGCCAAGAGTCCTGCCGAAGCCAAATCTCCAGCGGAGGCCAAA TCACCTGCGGAGGCAAAAAGTCCAGCGACCGTGAAGTCTCCGGGCGAGGCTAAGTCACCATCCGAAGCTA AGTCTCCGGCCGAGGCTAAATCCCCAGCAGAAGCAAAAAGTCCTGCGGAGGCTAAAAGCCCCGCTGAGGT GAAAAGTCCTGGCGAGGCCAAATCTCCTGCTGAGCCTAAGTCTCCTGCAGAGGCAAAATCCCCGGCTGAG GTCAAAAGCCCCGCGGAAGCTAAGTCCCCCGCTGAAGTAAAATCCCCTGGAGAAGCCAAGAGCCCCGCTG CCGTCAAGTCACCCGCGGAAGCTAAGTCTCCCGCTGCTGTCAAATCACCCGGGGAGGCTAAGTCACCTGG AGAGGCTAAGTCACCCGCAGAGGCAAAAAGCCCTGCCGAAGCTAAGTCCCCCATCGAAGTAAAGAGCCCT GAAAAGGCAAAGACCCCGGTGAAAGAGGGTGCCAAGTCCCCCGCCGAGGCAAAGTCCCCCGAGAAGGCAA AATCTCCTGTGAAGGAAGACATCAAGCCCCCCGCAGAGGCCAAAAGTCCAGAGAAGGCTAAGTCTCCCGT GAAAGAAGGGGCTAAACCACCAGAGAAGGCAAAGCCTCTGGACGTGAAGAGCCCAGAGGCACAGACACCA GTCCAGGAAGAGGCCAAACACCCTACGGACATTCGGCCGCCAGAACAGGTGAAGTCACCCGCAAAAGAGA AGGCCAAGAGTCCTGAGAAGGAAGAGGCTAAGACTTCCGAGAAGGTGGCTCCTAAAAAAGAGGAAGTAAA AAGTCCCGTCAAAGAAGAGGTGAAAGCTAAAGAGCCACCGAAGAAAGTCGAAGAAGAGAAAACTTTGCCT ACTCCCAAGACAGAGGCCAAGGAATCTAAAAAGGACGAAGCTCCTAAGGAGGCTCCAAAGCCCAAGGTCG AGGAAAAAAAGGAGACTCCAACAGAGAAGCCTAAAGACAGCACCGCCGAAGCCAAAAAGGAGGAGGCGGG CGAGAAGAAAAAGGCTGTTGCGAGCGAGGAGGAGACACCAGCAAAGCTGGGAGTGAAGGAAGAGGCCAAG CCCAAAGAAAAGACCGAAACAACAAAGACGGAGGCCGAGGACACTAAGGCTAAAGAACCAAGTAAACCCA CCGAAACTGAAAAGCCCAAAAAAGAGGAGATGCCCGCTGCTCCTGAAAAGAAGGATACCAAGGAGGAAAA AACTACCGAGAGCAGGAAGCCGGAGGAAAAGCCTAAGATGGAGGCCAAGGTCAAGGAAGACGATAAGTCC CTGTCCAAGGAACCGAGCAAGCCCAAAACGGAGAAGGCAGAAAAGAGCTCTTCTACAGATCAAAAAGAGT CCCAACCCCCAGAGAAAACCACAGAGGATAAGGCCACGAAGGGCGAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227134 representing NM_010904
Red=Cloning site Green=Tags(s) MMSFGSADALLGAPFAPLHGGGSLHYSLSRKAGPGGTRSAAGSSSGFHSWARTSVSSVSASPSRFRGAAS STDSLDTLSNGPEGCVVAAVAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAM GELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAA RVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHA VQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDR HQADIASYQDAIQQLDSELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFSL TEGLPKIPSISTHIKVKSEEMIKVVEKSEKETVIVEGQTEEIRVTEGVTEEEDKEAQGQEGEEAEEGEEK EEEEGAAATSPPAEEAASPEKETKSRVKEEAKSPGEAKSPGEAKSPAEAKSPGEAKSPGEAKSPGEAKSP AEPKSPAEPKSPAEAKSPAEPKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAK SPAEAKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEVKSPGEAKSPAEPKSPAEAKSPAE VKSPAEAKSPAEVKSPGEAKSPAAVKSPAEAKSPAAVKSPGEAKSPGEAKSPAEAKSPAEAKSPIEVKSP EKAKTPVKEGAKSPAEAKSPEKAKSPVKEDIKPPAEAKSPEKAKSPVKEGAKPPEKAKPLDVKSPEAQTP VQEEAKHPTDIRPPEQVKSPAKEKAKSPEKEEAKTSEKVAPKKEEVKSPVKEEVKAKEPPKKVEEEKTLP TPKTEAKESKKDEAPKEAPKPKVEEKKETPTEKPKDSTAEAKKEEAGEKKKAVASEEETPAKLGVKEEAK PKEKTETTKTEAEDTKAKEPSKPTETEKPKKEEMPAAPEKKDTKEEKTTESRKPEEKPKMEAKVKEDDKS LSKEPSKPKTEKAEKSSSTDQKESQPPEKTTEDKATKGEK TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010904 |
| ORF Size | 3270 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010904.3, NP_035034.2 |
| RefSeq Size | 4004 bp |
| RefSeq ORF | 3273 bp |
| Locus ID | 380684 |
| UniProt ID | P19246 |
| Gene Summary | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In vitro and in situ visualization of cytoskeletal deformation under load: traumatic axonal injury
,Fournier, AJ;Rajbhandari, L;Shrestha, S;Venkatesan, A;Ramesh, KT;,
FASEB J.
,PubMed ID 25205740
[NEFH]
|
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