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Cdh1 (NM_009864) Mouse Tagged ORF Clone
CAT＃: MG227372
- TrueORF®
Cdh1 (tGFP-tagged) - Mouse cadherin 1 (Cdh1), (10ug)
ORF Plasmid: DDK
"NM_009864" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA960649; ARC-1; E-ca; E-cad; E-cadh; Eca; Ecad; L-C; L-CAM; Um; UVO |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227372 representing NM_009864
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCCGGTGCCGCAGCTTTTCCGCGCTCCTGCTCCTGCTGCAGGTCTCCTCATGGCTTTGCCAGG AGCTGGAGCCTGAGTCCTGCAGTCCCGGCTTCAGTTCCGAGGTCTACACCTTCCCGGTGCCGGAGAGGCA CCTGGAGAGAGGCCATGTCCTGGGCAGAGTGAGATTTGAAGGATGCACCGGCCGGCCAAGGACAGCCTTC TTTTCGGAAGACTCCCGATTCAAAGTGGCGACAGACGGCACCATCACAGTGAAGCGGCATCTAAAGCTCC ACAAGCTGGAGACCAGTTTCCTCGTCCGCGCCCGGGACTCCAGTCATAGGGAGCTGTCTACCAAAGTGAC GCTGAAGTCCATGGGGCACCACCATCACCGGCACCACCACCGCGACCCTGCCTCTGAATCCAACCCAGAG CTGCTCATGTTTCCCAGCGTGTACCCAGGTCTCAGAAGACAGAAACGAGACTGGGTCATCCCTCCCATCA GCTGCCCCGAAAATGAAAAGGGTGAATTCCCAAAGAACCTGGTTCAGATCAAATCCAACAGGGACAAAGA AACAAAGGTTTTCTACAGCATCACCGGCCAAGGAGCTGACAAACCCCCCGTTGGCGTTTTCATCATTGAG AGGGAGACAGGCTGGCTGAAAGTGACACAGCCTCTGGATAGAGAAGCCATTGCCAAGTACATCCTCTATT CTCATGCCGTGTCATCAAATGGGGAAGCGGTGGAGGATCCCATGGAGATAGTGATCACAGTGACAGATCA GAATGACAACAGGCCAGAGTTTACCCAGGAGGTGTTTGAGGGATCCGTTGCAGAAGGCGCTGTTCCAGGA ACCTCCGTGATGAAGGTCTCAGCCACCGATGCAGACGATGACGTCAACACCTACAACGCTGCCATCGCCT ACACCATCGTCAGCCAGGATCCTGAGCTGCCTCACAAAAACATGTTCACTGTCAATAGGGACACCGGGGT CATCAGTGTGCTCACCTCTGGGCTGGACCGAGAGAGTTACCCTACATACACTCTGGTGGTTCAGGCTGCT GACCTTCAAGGCGAAGGCTTGAGCACAACAGCCAAGGCTGTGATCACTGTCAAGGATATTAATGACAACG CTCCTGTCTTCAACCCGAGCACGTATCAGGGTCAAGTGCCTGAGAATGAGGTCAATGCCCGGATCGCCAC ACTCAAAGTGACCGATGATGATGCCCCCAACACTCCGGCGTGGAAAGCTGTGTACACCGTAGTCAACGAT CCTGACCAGCAGTTCGTTGTCGTCACAGACCCCACGACCAATGATGGCATTTTGAAAACAGCCAAGGGCT TGGATTTTGAGGCCAAGCAGCAATACATCCTTCATGTGAGAGTGGAGAACGAGGAACCCTTTGAGGGGTC TCTTGTCCCTTCCACAGCCACTGTCACTGTGGACGTGGTAGACGTGAATGAAGCCCCCATCTTTATGCCT GCGGAGAGGAGAGTCGAAGTGCCCGAAGACTTTGGTGTGGGTCAGGAAATCACATCTTATACCGCTCGAG AGCCGGACACGTTCATGGATCAGAAGATCACGTATCGGATTTGGAGGGACACTGCCAACTGGCTGGAGAT TAACCCAGAGACTGGTGCCATTTTCACGCGCGCTGAGATGGACAGAGAAGACGCTGAGCATGTGAAGAAC AGCACATATGTAGCTCTCATCATCGCCACAGATGATGGTTCACCCATTGCCACTGGCACGGGCACTCTTC TCCTGGTCCTGTTAGACGTCAATGATAACGCTCCCATCCCAGAACCTCGAAACATGCAGTTCTGCCAGAG GAACCCACAGCCTCATATCATCACCATCTTGGATCCAGACCTTCCCCCCAACACGTCCCCCTTTACTGCT GAGCTAACCCATGGGGCCAGCGTCAACTGGACCATTGAGTATAATGACGCAGCTCAAGAATCTCTCATTT TGCAACCAAGAAAGGACTTAGAGATTGGCGAATACAAAATCCATCTCAAGCTCGCGGATAACCAGAACAA AGACCAGGTGACCACGTTGGACGTCCATGTGTGTGACTGTGAAGGGACGGTCAACAACTGCATGAAGGCG GGAATCGTGGCAGCAGGATTGCAAGTTCCTGCCATCCTCGGAATCCTTGGAGGGATCCTCGCCCTGCTGA TTCTGATCCTGCTGCTCCTACTGTTTCTACGGAGGAGAACGGTGGTCAAAGAGCCCCTGCTGCCACCAGA TGATGATACCCGGGACAATGTGTATTACTATGATGAAGAAGGAGGTGGAGAAGAAGACCAGGACTTTGAT TTGAGCCAGCTGCACAGGGGCCTGGATGCCCGACCGGAAGTGACTCGAAATGATGTGGCTCCCACCCTCA TGAGCGTGCCCCAGTATCGTCCCCGTCCTGCCAATCCTGATGAAATTGGAAACTTCATCGATGAAAACCT GAAGGCAGCCGACAGCGACCCCACGGCACCCCCTTACGACTCTCTGTTGGTGTTCGATTACGAGGGCAGT GGTTCTGAAGCCGCTAGCCTGAGCTCACTGAACTCCTCTGAGTCGGATCAGGACCAGGACTACGATTATC TGAACGAGTGGGGCAACCGATTCAAGAAGCTGGCGGACATGTACGGCGGTGGCGAGGACGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227372 representing NM_009864
Red=Cloning site Green=Tags(s) MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVPERHLERGHVLGRVRFEGCTGRPRTAF FSEDSRFKVATDGTITVKRHLKLHKLETSFLVRARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPE LLMFPSVYPGLRRQKRDWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIE RETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPG TSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAA DLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVND PDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMP AERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKN STYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTA ELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKA GIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVKEPLLPPDDDTRDNVYYYDEEGGGEEDQDFD LSQLHRGLDARPEVTRNDVAPTLMSVPQYRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGS GSEAASLSSLNSSESDQDQDYDYLNEWGNRFKKLADMYGGGEDD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009864 |
| ORF Size | 2652 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009864.2, NP_033994.1 |
| RefSeq Size | 4413 bp |
| RefSeq ORF | 2655 bp |
| Locus ID | 12550 |
| UniProt ID | P09803 |
| Gene Summary | This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8. [provided by RefSeq, Oct 2015] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222365 | Cdh1 (untagged) - Mouse cadherin 1 (Cdh1), (10ug) |
CNY 11,310.00 |
|
| MR227372 | Cdh1 (Myc-DDK-tagged) - Mouse cadherin 1 (Cdh1) |
CNY 6,472.00 |
|
| MR227372L3 | Lenti ORF clone of Cdh1 (Myc-DDK-tagged) - Mouse cadherin 1 (Cdh1) |
CNY 8,872.00 |
|
| MR227372L4 | Lenti ORF clone of Cdh1 (mGFP-tagged) - Mouse cadherin 1 (Cdh1) |
CNY 9,030.00 |
