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Ace (NM_009598) Mouse Tagged ORF Clone
CAT＃: MG227395
- TrueORF®
Ace (tGFP-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_009598" in other vectors (3)
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CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW208573; CD143 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227395 representing NM_009598
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAAGGTTGGGCTACTCCAGGACTGCCCAGCTTCCTCTTTCTGCTGCTCTGCTGTGGGCACCATC TGCTGGTCCTGAGTCAGGTGGCCACTGACCACGTGACAGCCAACCAGGGGATAACAAACCAGGCAACAAC CAGAAGCCAGACAACAACTCACCAAGCAACCATTGACCAGACAACCCAGATCCCAAACCTAGAGACTGAT GAAGCCAAGGCTGACAGGTTCGTGGAAGAGTATGACCGGACAGCCCAAGTGTTGTTGAACGAGTACGCAG AGGCCAACTGGCAATATAACACCAACATTACCATAGAGGGCAGCAAGATCCTGCTTGAGAAAAGCACGGA GGTATCCAATCACACCCTGAAATATGGCACCCGGGCCAAGACATTTGATGTGAGCAACTTTCAAAACTCT TCCATCAAGCGGATCATAAAGAAGCTTCAGAACCTGGACCGGGCAGTGCTGCCTCCCAAGGAATTAGAAG AGTACAACCAGATCCTGCTAGACATGGAGACAACTTACAGCTTATCCAACATTTGCTACACAAATGGCAC TTGTATGCCCCTGGAACCTGATCTAACAAACATGATGGCCACATCCCGGAAATATGAAGAATTGCTATGG GCATGGAAGAGCTGGAGAGACAAGGTGGGGAGAGCCATCCTTCCTTTTTTCCCAAAGTATGTGGAGTTCT CCAACAAGATTGCCAAGCTCAATGGCTACACGGATGCAGGGGATTCATGGAGATCCTTATACGAGTCTGA CAACCTGGAGCAAGACCTGGAAAAACTGTACCAGGAGCTGCAGCCACTCTACCTGAACCTGCATGCCTAT GTGCGTCGTTCCCTGCACCGCCACTATGGGTCCGAGTACATCAACCTGGATGGCCCCATTCCTGCCCATC TGCTAGGGAACATGTGGGCGCAGACCTGGTCCAACATCTATGATTTGGTGGCGCCCTTCCCTTCCGCCCC CAATATAGATGCCACGGAAGCCATGATAAAGCAGGGATGGACACCCAGAAGGATATTTAAGGAAGCTGAC AATTTCTTTACCTCCCTGGGGCTGTTACCTGTGCCCCCTGAGTTCTGGAACAAGTCGATGTTAGAGAAGC CCACCGATGGAAGGGAGGTGGTGTGCCATCCCTCAGCCTGGGACTTCTACAACGGCAAGGACTTCAGGAT CAAGCAGTGTACCTCTGTGAACATGGAGGACTTGGTGATAGCGCACCACGAAATGGGCCACATCCAGTAT TTCATGCAGTACAAAGACTTACCCGTGACTTTCCGGGAGGGTGCCAACCCTGGTTTTCATGAAGCTATTG GAGATATAATGGCTCTCTCAGTGTCTACCCCCAAGCATCTATACAGTCTCAACCTGCTTAGCACTGAGGG CAGTGGCTACGAGTATGACATCAACTTTCTAATGAAGATGGCCCTCGACAAGATCGCCTTTATCCCCTTC AGCTACCTCATCGACCAGTGGCGCTGGAGGGTCTTTGATGGAAGCATCACCAAGGAGAACTATAACCAGG AGTGGTGGAGCCTCAGGCTGAAGTATCAGGGTCTGTGCCCCCCAGTGCCAAGATCCCAAGGTGACTTTGA CCCAGGGTCCAAGTTCCACGTTCCTGCGAACGTGCCATACGTCAGGTACTTTGTCAGCTTCATCATCCAG TTCCAGTTCCACGAGGCGCTGTGTCGCGCAGCCGGGCACACGGGTCCCCTGCACAAGTGTGACATCTACC AATCCAAGGAAGCAGGGAAGCTCCTGGCGGATGCCATGAAGCTGGGCTACAGTAAGCCGTGGCCAGAGGC CATGAAGCTGATCACAGGCCAGCCTAACATGTCAGCCTCCGCCATGATGAATTACTTCAAGCCACTGACA GAATGGCTCGTCACCGAGAACAGGAGACATGGAGAGACACTGGGCTGGCCGGAGTACAACTGGGCGCCAA ACACCGCTCGCGCAGAAGGCTCCACCGCAGAGTCCAACCGCGTCAATTTCCTGGGCCTGTACCTGGAGCC ACAGCAGGCCCGCGTGGGCCAGTGGGTGCTGCTCTTCCTGGGCGTCGCCCTGCTCGTGGCCACCGTGGGT CTCGCCCATCGGCTCTACAACATCCGTAACCATCACAGCCTCCGCCGGCCCCACCGTGGGCCCCAGTTTG GGTCTGAGGTGGAGCTCAGACACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227395 representing NM_009598
Red=Cloning site Green=Tags(s) MGQGWATPGLPSFLFLLLCCGHHLLVLSQVATDHVTANQGITNQATTRSQTTTHQATIDQTTQIPNLETD EAKADRFVEEYDRTAQVLLNEYAEANWQYNTNITIEGSKILLEKSTEVSNHTLKYGTRAKTFDVSNFQNS SIKRIIKKLQNLDRAVLPPKELEEYNQILLDMETTYSLSNICYTNGTCMPLEPDLTNMMATSRKYEELLW AWKSWRDKVGRAILPFFPKYVEFSNKIAKLNGYTDAGDSWRSLYESDNLEQDLEKLYQELQPLYLNLHAY VRRSLHRHYGSEYINLDGPIPAHLLGNMWAQTWSNIYDLVAPFPSAPNIDATEAMIKQGWTPRRIFKEAD NFFTSLGLLPVPPEFWNKSMLEKPTDGREVVCHPSAWDFYNGKDFRIKQCTSVNMEDLVIAHHEMGHIQY FMQYKDLPVTFREGANPGFHEAIGDIMALSVSTPKHLYSLNLLSTEGSGYEYDINFLMKMALDKIAFIPF SYLIDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRSQGDFDPGSKFHVPANVPYVRYFVSFIIQ FQFHEALCRAAGHTGPLHKCDIYQSKEAGKLLADAMKLGYSKPWPEAMKLITGQPNMSASAMMNYFKPLT EWLVTENRRHGETLGWPEYNWAPNTARAEGSTAESNRVNFLGLYLEPQQARVGQWVLLFLGVALLVATVG LAHRLYNIRNHHSLRRPHRGPQFGSEVELRHS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009598 |
| ORF Size | 2196 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009598.3 |
| RefSeq Size | 3129 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 11421 |
| UniProt ID | P09470 |
| Gene Summary | Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR227395 | Ace (Myc-DDK-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), transcript variant 2 |
CNY 5,360.00 |
|
| MR227395L3 | Lenti ORF clone of Ace (Myc-DDK-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), transcript variant 2 |
CNY 8,360.00 |
|
| MR227395L4 | Lenti ORF clone of Ace (mGFP-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), transcript variant 2 |
CNY 8,360.00 |
