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Mmp13 (NM_008607) Mouse Tagged ORF Clone
CAT＃: MG227427
- TrueORF®
Mmp13 (tGFP-tagged) - Mouse matrix metallopeptidase 13 (Mmp13), (10ug)
ORF Plasmid: DDK
"NM_008607" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cl; Clg; Mmp; MMP-1; MMP-13; Mmp1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227427 representing NM_008607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATTCAGCTATCCTGGCCACCTTCTTCTTGTTGAGCTGGACTCCCTGTTGGTCCCTGCCCCTTCCCT ATGGTGATGATGATGATGATGACCTGTCTGAGGAAGACCTTGTGTTTGCAGAGCACTACTTGAAATCATA CTACCATCCTGCGACTCTTGCGGGAATCCTGAAGAAGTCTACAGTGACCTCCACAGTTGACAGGCTCCGA GAAATGCAATCTTTCTTTGGCTTAGAGGTGACTGGCAAACTTGATGATCCCACCTTAGACATCATGAGAA AACCAAGATGTGGAGTGCCTGATGTGGGTGAATACAATGTTTTCCCTAGAACACTCAAATGGTCCCAAAC GAACTTAACTTACAGGATTGTGAACTATACTCCTGATATGTCCCATTCTGAAGTGGAGAAGGCCTTCAGA AAAGCCTTCAAGGTCTGGTCTGATGTGACACCACTGAATTTCACCAGAATCTATGATGGCACTGCTGACA TCATGATATCTTTTGGGACTAAAGAACATGGTGACTTCTACCCATTTGATGGACCTTCTGGTCTTCTGGC ACACGCTTTTCCTCCTGGACCAAACTATGGTGGGGATGCCCATTTTGATGATGATGAAACCTGGACAAGC AGTTCCAAAGGCTACAACTTGTTTATTGTTGCTGCCCATGAGCTTGGCCACTCCCTAGGTCTGGATCACT CCAAGGACCCAGGAGCCCTGATGTTTCCCATCTATACCTACACTGGCAAAAGCCATTTCATGCTTCCTGA TGATGACGTTCAAGGAATTCAGTTTCTTTATGGTCCAGGCGATGAAGACCCCAACCCTAAGCATCCCAAA ACACCAGAGAAGTGTGACCCAGCCCTATCCCTTGATGCCATTACCAGTCTCCGAGGAGAAACTATGATCT TTAAAGACAGATTCTTCTGGCGCCTGCACCCTCAGCAGGTTGAGGCTGAGCTCTTTTTGACAAAGTCCTT TTGGCCAGAACTTCCCAACCATGTGGATGCTGCATATGAACATCCATCCCGTGACCTTATGTTTATCTTT AGAGGGAGAAAATTCTGGGCTCTGAATGGTTATGACATTCTGGAAGGTTATCCCAGAAAAATATCTGACC TGGGATTCCCAAAAGAGGTGAAGAGACTGAGCGCTGCGGTTCACTTTGAGAACACGGGGAAGACCCTCTT CTTCTCTGAGAACCACGTGTGGAGTTATGATGATGTTAACCAGACTATGGACAAAGATTATCCCCGCCTC ATAGAAGAGGAATTCCCTGGAATTGGCAACAAAGTAGATGCTGTCTATGAGAAAAATGGCTATATCTACT TTTTCAATGGGCCCATACAGTTTGAATACAGTATCTGGAGTAATCGCATTGTGAGAGTCATGCCAACAAA TTCCATATTGTGGTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227427 representing NM_008607
Red=Cloning site Green=Tags(s) MHSAILATFFLLSWTPCWSLPLPYGDDDDDDLSEEDLVFAEHYLKSYYHPATLAGILKKSTVTSTVDRLR EMQSFFGLEVTGKLDDPTLDIMRKPRCGVPDVGEYNVFPRTLKWSQTNLTYRIVNYTPDMSHSEVEKAFR KAFKVWSDVTPLNFTRIYDGTADIMISFGTKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTS SSKGYNLFIVAAHELGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQFLYGPGDEDPNPKHPK TPEKCDPALSLDAITSLRGETMIFKDRFFWRLHPQQVEAELFLTKSFWPELPNHVDAAYEHPSRDLMFIF RGRKFWALNGYDILEGYPRKISDLGFPKEVKRLSAAVHFENTGKTLFFSENHVWSYDDVNQTMDKDYPRL IEEEFPGIGNKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPTNSILWC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008607 |
| ORF Size | 1416 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008607.2, NP_032633.1 |
| RefSeq Size | 2675 bp |
| RefSeq ORF | 1419 bp |
| Locus ID | 17386 |
| UniProt ID | P33435 |
| Gene Summary | This gene encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Jun 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216470 | Mmp13 (untagged) - Mouse matrix metallopeptidase 13 (Mmp13), (10ug) |
CNY 5,856.00 |
|
| MR227427 | Mmp13 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 13 (Mmp13) |
CNY 5,488.00 |
|
| MR227427L3 | Lenti ORF clone of Mmp13 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 13 (Mmp13) |
CNY 6,650.00 |
|
| MR227427L4 | Lenti ORF clone of Mmp13 (mGFP-tagged) - Mouse matrix metallopeptidase 13 (Mmp13) |
CNY 6,650.00 |
