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Hsd17b2 (NM_008290) Mouse Tagged ORF Clone
CAT＃: MG231988
- TrueORF®
Hsd17b2 (GFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2)
ORF Plasmid: DDK
"NM_008290" in other vectors (3)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Tag | Myc-DDK |
| Synonyms | AI194836; AI194967; AI255511 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG231988 representing NM_008290.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCCCGTTTGCCTCTGAGTCAGCATGGCTCTGCTTGGCTGCCGCTGCAGTGCTGGGGGGCACGCTT CTCTGCGGATGCCGGAGTGGCAGGCAGCTGCGGAGCCAGGCGGTGTGTCTCGCTGGCCTCTGGGGAGGT GCCTGCCTGCTCAGTCTGTCCCTCCTCTGCACCTTGTTCCTCTTATCCGTGGCCTGCTTCCTTCTTCTG TATATGAGTTCATCTGACCAAGACTTGTTACCTGTGGATCAAAAGGCCGTGCTGGTGACAGGTGCTGAT TCCGGTTTCGGTCACGGTTTGGCCAAGCACCTGGACAAGCTGGGTTTCACAGTGTTTGCTGGAGTGCTG GACAAAGAGGGCCCGGGAGCAGAGGAACTGAGAAAACACTGCTCTGAGCGTCTTTCAGTGCTCCAGATG GATGTCACCAAGCCAGAGCAGATAAAAGATGCTCACAGCAAAGTCACAGAGAAGATACAAGACAAAGGA CTGTGGGCCGTGGTTAACAATGCCGGGGTCTTCCACTTACCTATCGACGGGGAACTCATACCCATGAGC ATCTACAGAAAATGCATGGCCGTGAACTTCTTCGGAACTGTCGAGGTCACCAAGGCGTTTCTGCCTCTA CTTAGAAAGTCCAAGGGGAGGCTGGTGAACGTCAGCAGCATGGGAGGCACGGTTCCACTTCAAATGACA TCAGCTTATGCAGCCACAAAGGCAGCTCTAACCATGTTCTCAACAATCATCAGACAGGAGCTTGACAAA TGGGGAGTTAAAGTTGTGACCATCAAACCCGGAGGCTTCAAAACAAACATCACAGGCTCACAGGACATT TGGGATAAAATGGAGAAGGAAATCCTGGACCACTTTTCCAAAGATATTCAGGAGAACTATGGCCAGGAC TACGTCCATACACAGAAGCTCATCATCCCGACCTTGAAAGAGAGGTCCAACCCGGACATCACCCCTGTG CTTCGGGACATCCAGCATGCCATTTCTGCAAGGAACCCCTCTTCCTTTTATTACCCAGGGCGAATGGCT TACCTGTGGGTCTGCTTAGCAGCCTACTGCCCCACCAGCTTACTTGACTATGTTATAAAAAAAGGTTTT TATCCACAACCCACGCCGAGAGCTTTAAGGACAGTGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by MG231988
Blue=ORF Red=Cloning site Green=Tag(s) MSPFASESAWLCLAAAAVLGGTLLCGCRSGRQLRSQAVCLAGLWGGACLLSLSLLCTLFLLSVACFLLL YMSSSDQDLLPVDQKAVLVTGADSGFGHGLAKHLDKLGFTVFAGVLDKEGPGAEELRKHCSERLSVLQM DVTKPEQIKDAHSKVTEKIQDKGLWAVVNNAGVFHLPIDGELIPMSIYRKCMAVNFFGTVEVTKAFLPL LRKSKGRLVNVSSMGGTVPLQMTSAYAATKAALTMFSTIIRQELDKWGVKVVTIKPGGFKTNITGSQDI WDKMEKEILDHFSKDIQENYGQDYVHTQKLIIPTLKERSNPDITPVLRDIQHAISARNPSSFYYPGRMA YLWVCLAAYCPTSLLDYVIKKGFYPQPTPRALRTVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008290 |
| ORF Size | 1143 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008290.2, NP_032316.2 |
| RefSeq Size | 1280 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 15486 |
| UniProt ID | P51658 |
| MW | 41.8 kDa |
| Gene Summary | Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR231988 | Hsd17b2 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 3,656.00 |
|
| MR231988L3 | Lenti ORF clone of Hsd17b2 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 5,890.00 |
|
| MR231988L4 | Lenti ORF clone of Hsd17b2 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 2 (Hsd17b2) |
CNY 5,890.00 |
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