Rheb (NM_053075) Mouse Tagged ORF Clone
CAT#: MR201682
- TrueORF®
Rheb (Myc-DDK-tagged) - Mouse Ras homolog enriched in brain (Rheb)
Lentiviral Particles: mGFP
"NM_053075" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Rheb1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201682 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCAGTCCAAGTCCCGGAAGATCGCCATCCTGGGCTACCGGTCTGTGGGAAAGTCCTCATTGACAA TTCAGTTTGTTGAAGGCCAATTTGTTGATTCCTACGATCCAACCATAGAGAACACGTTCACCAAGTTGAT CACGGTAAATGGTCAAGAGTATCATCTTCAGCTTGTAGACACAGCGGGGCAGGATGAATATTCCATTTTT CCTCAGACATACTCCATAGATATTAATGGTTATATTCTTGTGTATTCTGTTACATCAATCAAAAGTTTTG AAGTAATTAAAGTTATCCATGGCAAGTTGTTGGATATGGTGGGGAAAGTGCAGATACCTATTATGTTGGT TGGAAATAAGAAGGACCTGCATATGGAAAGGGTGATCAGCTATGAAGAAGGAAAGGCTTTGGCAGAATCT TGGAATGCAGCTTTTTTGGAATCTTCTGCTAAAGAAAATCAAACTGCTGTTGATGTTTTTAAAAGGATAA TTTTGGAAGCAGAAAAGATTGATGGAGCAGCTTCACAAGGAAAGTCTTCGTGCTCGGTGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201682 protein sequence
Red=Cloning site Green=Tags(s) MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIF PQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAES WNAAFLESSAKENQTAVDVFKRIILEAEKIDGAASQGKSSCSVM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_053075 |
ORF Size | 555 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053075.3, NP_444305.2 |
RefSeq Size | 1521 bp |
RefSeq ORF | 555 bp |
Locus ID | 19744 |
UniProt ID | Q921J2 |
MW | 20.5 kDa |
Gene Summary | Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo
,Kim, S;Moon, GJ;Oh, YS;Park, J;Shin, WH;Jeong, JY;Choi, KS;Jin, BK;Kholodilov, N;Burke, RE;Kim, HJ;Ha, CM;Lee, SG;Kim, SR;,
Exp. Mol. Med.
,PubMed ID 29422542
[RHEB]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200944 | Rheb (untagged) - Mouse Ras homolog enriched in brain (Rheb), (10ug) |
CNY 2,000.00 |
|
MR201682L2 | Lenti ORF clone of Rheb (mGFP-tagged) - Mouse Ras homolog enriched in brain (Rheb) |
CNY 4,800.00 |
|
MR201682L3 | Lenti ORF clone of Rheb (Myc-DDK-tagged) - Mouse Ras homolog enriched in brain (Rheb) |
CNY 4,750.00 |
|
MR201682L4 | Lenti ORF clone of Rheb (mGFP-tagged) - Mouse Ras homolog enriched in brain (Rheb) |
CNY 4,750.00 |