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Rpl13a (NM_009438) Mouse Tagged ORF Clone
CAT＃: MR202103
- TrueORF®
Rpl13a (Myc-DDK-tagged) - Mouse ribosomal protein L13A (Rpl13a)
ORF Plasmid: tGFP
"NM_009438" in other vectors (4)
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CNY 2,081.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810026N22Rik; Tstap198-7; tum-antigen |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202103 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGGGCAGGTTCTGGTATTGGATGGCCGAGGCCATCTTCTTGGCCGCCTGGCGGCCATTGTGG CCAAGCAGGTACTTCTGGGCCGGAAGGTGGTGGTCGTACGCTGTGAAGGCATCAACATTTCTGGAAACTT CTACAGAAACAAGTTAAAGTATCTGGCCTTTCTCCGGAAGCGGATGAATACCAACCCCTCCCGAGGCCCC TACCACTTCCGAGCCCCCAGCCGCATTTTCTGGCGCACTGTGCGAGGCATGCTGCCCCACAAGACCAAGA GAGGCCAGGCTGCCCTGGAGCGCCTCAAGGTGTTGGATGGGATCCCTCCACCCTATGACAAGAAAAAGCG GATGGTGGTCCCTGCTGCTCTCAAGGTTGTTCGGCTGAAGCCTACCAGAAAGTTTGCTTACCTGGGGCGT CTGGCGCATGAGGTCGGGTGGAAGTACCAGGCAGTGACAGCCACTCTGGAGGAGAAACGGAAGGAAAAGG CCAAGATGCACTATCGGAAGAAGAAGCAGATCTTGAGGTTACGGAAACAGGCAGAAAAGAATGTGGAGAA GAAAATCTGCAAGTTCACAGAGGTCCTCAAGACCAACGGACTCCTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202103 protein sequence
Red=Cloning site Green=Tags(s) MAEGQVLVLDGRGHLLGRLAAIVAKQVLLGRKVVVVRCEGINISGNFYRNKLKYLAFLRKRMNTNPSRGP YHFRAPSRIFWRTVRGMLPHKTKRGQAALERLKVLDGIPPPYDKKKRMVVPAALKVVRLKPTRKFAYLGR LAHEVGWKYQAVTATLEEKRKEKAKMHYRKKKQILRLRKQAEKNVEKKICKFTEVLKTNGLLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009438 |
| ORF Size | 612 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009438.5 |
| RefSeq Size | 1039 bp |
| RefSeq ORF | 612 bp |
| Locus ID | 22121 |
| UniProt ID | P19253 |
| MW | 23.5 kDa |
| Gene Summary | Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3' UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress
,Kobiita, A;Godbersen, S;Araldi, E;Ghoshdastider, U;Schmid, MW;Spinas, G;Moch, H;Stoffel, M;,
Cell Rep
,PubMed ID 32640216
[RPL13A]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202752 | Rpl13a (untagged) - Mouse ribosomal protein L13A (Rpl13a), (10ug) |
CNY 2,000.00 |
|
| MG202103 | Rpl13a (tGFP-tagged) - Mouse ribosomal protein L13a (Rpl13a) |
CNY 2,850.00 |
|
| MR202103L3 | Lenti ORF clone of Rpl13a (Myc-DDK-tagged) - Mouse ribosomal protein L13A (Rpl13a) |
CNY 4,750.00 |
|
| MR202103L4 | Lenti ORF clone of Rpl13a (mGFP-tagged) - Mouse ribosomal protein L13A (Rpl13a) |
CNY 4,750.00 |
