Il1a (NM_010554) Mouse Tagged ORF Clone
CAT#: MR203602
- TrueORF®
Il1a (Myc-DDK-tagged) - Mouse interleukin 1 alpha (Il1a)
ORF Plasmid: tGFP
"NM_010554" in other vectors (3)
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CNY 1,416.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Il-1a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203602 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAAGTTCCTGACTTGTTTGAAGACCTAAAGAACTGTTACAGTGAAAATGAAGACTACAGTTCTG CCATTGACCATCTCTCTCTGAATCAGAAATCCTTCTATGATGCAAGCTATGGCTCACTTCATGAGACTTG CACAGACCAGTTTGTATCTCTGAGAACCTCTGAAACGTCAAAGATGTCCAACTTCACCTTCAAGGAGAGC CGGGTGACAGTATCAGCAACGTCAAGCAACGGGAAGATTCTGAAGAAGAGACGGCTGAGTTTCAGTGAGA CCTTCACTGAAGATGACCTGCAGTCCATAACCCATGATCTGGAAGAGACCATCCAACCCAGATCAGCACC TTACACCTACCAGAGTGATTTGAGATACAAACTGATGAAGCTCGTCAGGCAGAAGTTTGTCATGAATGAT TCCCTCAACCAAACTATATATCAGGATGTGGACAAACACTATCTCAGCACCACTTGGTTAAATGACCTGC AACAGGAAGTAAAATTTGACATGTATGCCTACTCGTCGGGAGGAGATGACTCTAAATATCCTGTTACTCT AAAAATCTCAGATTCACAACTGTTCGTGAGCGCTCAAGGAGAAGACCAGCCCGTGTTGCTGAAGGAGTTG CCAGAAACACCAAAACTCATCACAGGTAGTGAGACCGACCTCATTTTCTTCTGGAAAAGTATCAACTCTA AGAACTACTTCACATCAGCTGCTTATCCAGAGCTGTTTATTGCCACCAAAGAACAAAGTCGGGTGCACCT GGCACGGGGACTGCCCTCTATGACAGACTTCCAGATATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203602 protein sequence
Red=Cloning site Green=Tags(s) MAKVPDLFEDLKNCYSENEDYSSAIDHLSLNQKSFYDASYGSLHETCTDQFVSLRTSETSKMSNFTFKES RVTVSATSSNGKILKKRRLSFSETFTEDDLQSITHDLEETIQPRSAPYTYQSDLRYKLMKLVRQKFVMND SLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKEL PETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010554 |
ORF Size | 813 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010554.2, NM_010554.3, NM_010554.4, NP_034684.2 |
RefSeq Size | 1974 bp |
RefSeq ORF | 813 bp |
Locus ID | 16175 |
UniProt ID | P01582 |
MW | 31 kDa |
Gene Summary | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Uncoupling the senescence-associated secretory phenotype from cell cycle exit via IL-1 inactivation unveils its pro-tumorigenic role
,Lau, L;Porciuncula, A;Yu, A;Iwakura, Y;David, G;,
Mol. Cell. Biol.
,PubMed ID 30988157
[IL1A]
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Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG203602 | Il1a (tGFP-tagged) - Mouse interleukin 1 alpha (Il1a) |
CNY 2,850.00 |
|
MR203602L3 | Lenti ORF clone of Il1a (Myc-DDK-tagged) - Mouse interleukin 1 alpha (Il1a) |
CNY 4,750.00 |
|
MR203602L4 | Lenti ORF clone of Il1a (mGFP-tagged) - Mouse interleukin 1 alpha (Il1a) |
CNY 4,750.00 |