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Prkra (NM_011871) Mouse Tagged ORF Clone
CAT＃: MR204457
- TrueORF®
Prkra (Myc-DDK-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra)
ORF Plasmid: tGFP
"NM_011871" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV120107; lear; Pact; PRK; RAX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204457 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCATAGCAGGCATCGTGCCGAGGCCCCGCCGCTGCAGCGCGAGGACAGCGGGACCTTCAGTTTGG GCAAGATGATAACAGCTAAGCCTGGGAAAACACCGATTCAGGTATTGCACGAGTACGGCATGAAGACCAA GAACATTCCAGTTTATGAATGTGAAAGATCCGATGTGCAAGTACACGTGCCCACTTTCACCTTCAGAGTA ACCGTTGGTGACATAACTTGCACAGGTGAAGGTACAAGTAAGAAGCTGGCGAAGCACAGAGCTGCGGAGG CCGCCATAAACATTTTGAAAGCCAATGCAAGTATTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGATCC ATCCAAACAGCCGAAGAACCAGCTGAATCCAATTGGCTCATTACAGGAATTAGCAATTCACCATGGCTGG CGACTTCCTGAATATACCCTTTCCCAGGAAGGAGGACCGGCTCATAAGAGGGAATACACCACGATCTGCA GGCTTGAATCATTCATGGAAACTGGAAAGGGGGCATCAAAAAAACAAGCCAAGAGAAATGCTGCTGAGAA ATTTCTCGCCAAGTTTAGTAATATTTCTCCAGAGAACCACATTTCTCTAACGAACGTGGTTGGACATTCC CTAGGATGCACTTGGCACTCCTTGAGGAATTCCCCTGGTGAGAAGATCAACCTCCTGAAAAGGAGCCTCC TCAGTCTCCCGAACACAGACTACATCCAGCTGCTTAGTGAGATTGCCAGCGAGCAAGGCTTTAACATAAC GTATTTGGATATAGAGGAGCTGAGTGCCAACGGACAGTATCAGTGTCTCGCAGAGCTGTCCACCAGTCCT ATCACCGTGTGTCACGGCTCAGGCATCTCCTGTGGCAATGCACAGAGTGACGCTGCTCACAATGCTCTGC AGTATTTAAAGATAATAGCAGAAAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204457 protein sequence
Red=Cloning site Green=Tags(s) MSHSRHRAEAPPLQREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQVHVPTFTFRV TVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGW RLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHS LGCTWHSLRNSPGEKINLLKRSLLSLPNTDYIQLLSEIASEQGFNITYLDIEELSANGQYQCLAELSTSP ITVCHGSGISCGNAQSDAAHNALQYLKIIAERK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011871 |
| ORF Size | 942 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011871.3 |
| RefSeq Size | 1554 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 23992 |
| UniProt ID | Q9WTX2 |
| MW | 34.4 kDa |
| Gene Summary | Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 (By similarity). Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203800 | Prkra (untagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), (10ug) |
CNY 2,000.00 |
|
| MG204457 | Prkra (tGFP-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) |
CNY 2,850.00 |
|
| MR204457L3 | Lenti ORF clone of Prkra (Myc-DDK-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) |
CNY 4,750.00 |
|
| MR204457L4 | Lenti ORF clone of Prkra (mGFP-tagged) - Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra) |
CNY 4,750.00 |
