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Slc9a3r1 (NM_012030) Mouse Tagged ORF Clone
CAT＃: MR205416
- TrueORF®
Slc9a3r1 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Slc9a3r1)
ORF Plasmid: tGFP
"NM_012030" in other vectors (4)
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CNY 3,330.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EBP-50; NHE-RF; NHERF-1; NHERF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205416 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCGGACGCAGCGGCCGGGGAGCCTCTGCCCCGGCTCTGCTGCCTGGAGAAGGGTCCAAATGGCT ACGGCTTCCACCTGCACGGGGAGAAGGGCAAGGTGGGCCAGTTTATCCGTCTGGTAGAACCTGGCTCGCC GGCCGAGAAGTCGGGGTTGTTGGCTGGAGACCGATTGGTGGAGGTGAACGGTGAGAATGTGGAGAAGGAG ACGCATCAGCAGGTGGTGAGCCGCATCCGCGCAGCGCTGAACGCCGTGCGCCTGCTGGTGGTCGACCCGG AGACCGACGAGCGTCTCAAGAAGCTGGGCGTCTCGATCCGGGAAGAGCTGCTGCGCCCCCAGGAGAAGTC CGAACAAGCCGAGCCTCCAGCGGCCGCCGACACTCATGAGGCTGGGGACCAGAATGAGGCCGAAAAGAGC CACTTGCGCGAGCTCCGGCCCCGGCTCTGCACCATGAAGAAAGGCCCCAATGGCTATGGCTTCAACCTGC ACAGCGACAAGTCTAAGCCAGGCCAGTTCATCCGAGCAGTGGACCCAGACTCACCGGCGGAGGCGTCTGG ACTCCGGGCCCAGGACCGAATTGTGGAGGTCAATGGTGTCTGCATGGAGGGCAAGCAGCACGGGGACGTG GTGTCCGCCATCAAGGGTGGAGGTGATGAGGCCAAGCTGCTGGTGGTAGACAAGGAAACAGATGAGTTCT TCAAGAAGTGCAAAGTGATCCCATCCCAGGAGCACCTGGATGGCCCCTTGCCTGAGCCCTTCAGCAATGG AGAGATACAGAAGGAGAGCAGCCGTGAAGCCCTGGTGGAGCCAGCTTCAGAGAGCCCCAGGCCAGCCCTG GCAAGATCTGCCTCCAGCGATACCAGTGAGGAGCTAAATTCCCAAGACAGCCCCAAGAGACAAGTTTCCA CAGAGCCCTCATCTACCTCCTCCTCCTCCTCTGACCCCATCTTGGACCTCAACATCTCCTTGGCTGTGGC TAAAGAGAGGGCCCACCAGAAGCGCAGTAGCAAGAGGGCCCCGCAGATGGACTGGAGCAAGAAAAATGAA CTCTTCAGCAACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205416 protein sequence
Red=Cloning site Green=Tags(s) MSADAAAGEPLPRLCCLEKGPNGYGFHLHGEKGKVGQFIRLVEPGSPAEKSGLLAGDRLVEVNGENVEKE THQQVVSRIRAALNAVRLLVVDPETDERLKKLGVSIREELLRPQEKSEQAEPPAAADTHEAGDQNEAEKS HLRELRPRLCTMKKGPNGYGFNLHSDKSKPGQFIRAVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDV VSAIKGGGDEAKLLVVDKETDEFFKKCKVIPSQEHLDGPLPEPFSNGEIQKESSREALVEPASESPRPAL ARSASSDTSEELNSQDSPKRQVSTEPSSTSSSSSDPILDLNISLAVAKERAHQKRSSKRAPQMDWSKKNE LFSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012030 |
| ORF Size | 1068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012030.2, NP_036160.1 |
| RefSeq Size | 1922 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 26941 |
| UniProt ID | P70441 |
| MW | 38.6 kDa |
| Gene Summary | Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling (By similarity). May participate in HTR4 targeting to microvilli. Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity). Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Autophagy regulates testosterone synthesis by facilitating cholesterol uptake in Leydig cells
,Gao, F;Li, G;Liu, C;Gao, H;Wang, H;Liu, W;Chen, M;Shang, Y;Wang, L;Shi, J;Xia, W;Jiao, J;Gao, F;Li, J;Chen, L;Li, W;,
J. Cell Biol.
,PubMed ID 29618492
[SLC9A3R1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207466 | Slc9a3r1 (untagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Slc9a3r1), (10ug) |
CNY 3,990.00 |
|
| MG205416 | Slc9a3r1 (tGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1 (Slc9a3r1) |
CNY 3,710.00 |
|
| MR205416L3 | Lenti ORF clone of Slc9a3r1 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Slc9a3r1) |
CNY 5,230.00 |
|
| MR205416L4 | Lenti ORF clone of Slc9a3r1 (mGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Slc9a3r1) |
CNY 5,230.00 |
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