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Nfkbib (NM_010908) Mouse Tagged ORF Clone
CAT＃: MR205491
- TrueORF®
Nfkbib (Myc-DDK-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (Nfkbib)
ORF Plasmid: tGFP
"NM_010908" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | I(Kappa)B(beta); I-kappa-B-beta; Ik; IKapp; IKappaBbeta; IkB; ikB-B; IKB-beta; IkBb; NF-kappa-BIB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205491 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGTCGCGTGCTTGGGGAAAACTGCGGATGCCGATGAATGGTGCGACAGCGGCCTGGGCTCTC TAGGTCCCGACGCAGCGGCTCCCGGAGGACCAGGTCTGGGCGCAGAGCTTGGCCCAGAGCTGTCGTGGGC GCCCTTAGTCTTTGGCTACGTCACTGAGGATGGGGACACAGCCCTGCACTTGGCTGTGATTCATCAGCAT GAGCCCTTCCTGGATTTCCTCCTGGGCTTCTCCGCCGGCACCGAGTACCTTGACCTGCAGAATGACCTAG GCCAAACAGCCCTGCATCTAGCAGCCATCCTTGGGGAGGCATCTACAGTAGAGAAGTTGTATGCAGCCGG TGCAGGAGTGTTGGTGGCTGAGAGAGGGGGCCACACGGCATTGCACTTGGCCTGCCGGGTCAGGGCACAC ACGTGCGCGTGCGTACTGCTCCAGCCCCGTCCCAGCCACCCAAGAGATGCCTCAGATACCTACCTCACTC AGAGCCAGGACTGTACCCCAGACACCAGCCATGCCCCTGCTGCCGTGGATTCCCAACCCAACCCAGAGAA CGAAGAGGAGCCGCGTGATGAAGACTGGAGGCTACAACTAGAAGCTGAAAACTATGATGGCCATACCCCA CTCCATGTAGCTGTCATCCACAAAGATGCAGAGATGGTCCGGCTACTCAGGGATGCCGGAGCTGACCTCA ATAAACCGGAGCCTACGTGTGGCCGGACCCCTCTGCACCTGGCAGTAGAAGCCCAGGCAGCCAGCGTGCT GGAACTTCTCCTGAAAGCCGGTGCTGACCCCACCGCCCGCATGTATGGGGGCCGCACCCCACTTGGCAGT GCCCTGCTCCGGCCCAACCCCATCCTTGCCCGCCTCCTCCGTGCACATGGGGCCCCTGAACCTGAGGACG AGGACGATAAGCTTAGCCCTTGCAGCAGCAGCGGCAGCGACAGTGACAGTGACAACAGAGATGAGGGCGA TGAATATGATGACATCGTGGTTCACAGTGGCAGGAGCCAAAACCGACAACCGCCTTCCCCGGCATCCAAA CCTCTTCCTGATGACCCCAGCCCCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205491 protein sequence
Red=Cloning site Green=Tags(s) MAGVACLGKTADADEWCDSGLGSLGPDAAAPGGPGLGAELGPELSWAPLVFGYVTEDGDTALHLAVIHQH EPFLDFLLGFSAGTEYLDLQNDLGQTALHLAAILGEASTVEKLYAAGAGVLVAERGGHTALHLACRVRAH TCACVLLQPRPSHPRDASDTYLTQSQDCTPDTSHAPAAVDSQPNPENEEEPRDEDWRLQLEAENYDGHTP LHVAVIHKDAEMVRLLRDAGADLNKPEPTCGRTPLHLAVEAQAASVLELLLKAGADPTARMYGGRTPLGS ALLRPNPILARLLRAHGAPEPEDEDDKLSPCSSSGSDSDSDNRDEGDEYDDIVVHSGRSQNRQPPSPASK PLPDDPSPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010908 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010908.2, NM_010908.3, NM_010908.4, NM_010908.5, NP_035038.2 |
| RefSeq Size | 1945 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 18036 |
| UniProt ID | Q60778 |
| MW | 37.9 kDa |
| Gene Summary | This gene encodes an inhibitor of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB). The encoded protein prevents NF-kappaB-mediated transcription activation by sequestering it in the cytosol. In response to signals that induce NF-kappaB, such as cytokines and growth factors, the encoded protein undergoes phosphorylation, triggering its rapid ubiquitination and proteasomal degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201974 | Nfkbib (untagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (Nfkbib), (10ug) |
CNY 2,000.00 |
|
| MG205491 | Nfkbib (tGFP-tagged) - Mouse nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, beta (Nfkbib) |
CNY 3,710.00 |
|
| MR205491L3 | Lenti ORF clone of Nfkbib (Myc-DDK-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (Nfkbib) |
CNY 5,230.00 |
|
| MR205491L4 | Lenti ORF clone of Nfkbib (mGFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (Nfkbib) |
CNY 5,230.00 |
