United States
Germany
Japan
United Kingdom
China
Ednra (NM_010332) Mouse Tagged ORF Clone
CAT＃: MR206815
- TrueORF®
Ednra (Myc-DDK-tagged) - Mouse endothelin receptor type A (Ednra)
ORF Plasmid: tGFP
"NM_010332" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AEA001; ET-AR; ETa; Gpcr10; Mhdaaea1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATCTTTTGCCTTGCGGCATACTTTTGGCTGACCATGGTGGGAGGCGTAATGGCTGACAATCCGG AGAGATACAGCGCTAATCTAAGCAGCCACATGGAAGACTTCACCCCTTTTCCGGGGACGGAGATCAACTT TCTGGGCACCACCCATCGACCCCCTAATTTGGCCCTGCCTAGCAATGGCTCAATGCACGGCTATTGCCCA CAGCAGACTAAAATCACGACAGCTTTCAAATATATTAACACTGTGATATCCTGCACCATTTTCATCGTGG GAATGGTGGGGAACGCAACTCTACTACGAATCATTTACCAAAACAAGTGTATGAGGAACGGCCCCAATGC GCTCATAGCCAGCCTGGCCCTTGGAGACCTTATCTACGTGGTCATTGACCTCCCCATCAACGTGTTTAAG CTCTTGGCAGGACGCTGGCCTTTCGACCACAATGATTTTGGAGTGTTTCTCTGCAAGCTGTTCCCCTTCC TGCAGAAGTCCTCCGTGGGCATCACCGTCTTGAACCTCTGTGCTCTCAGTGTGGACAGGTACAGAGCAGT GGCTTCCTGGAGCCGAGTTCAAGGAATCGGGATCCCCTTGATTACCGCCATTGAAATCGTCTCCATCTGG ATTCTTTCCTTCATCTTGGCCATCCCGGAAGCAATCGGCTTCGTCATGGTACCCTTCGAATACAAGGGCG AGCTGCATAGGACCTGCATGCTCAACGCCACGTCCAAGTTCATGGAGTTTTACCAAGATGTGAAGGACTG GTGGCTCTTTGGGTTCTACTTCTGCATGCCCTTGGTGTGCACAGCAATCTTCTACACCCTCATGACCTGT GAGATGCTCAACAGGAGGAACGGCAGCTTGCGGATCGCCCTTAGTGAGCACCTCAAACAGCGTCGAGAAG TGGCAAAGACTGTCTTCTGCTTGGTTGTCATCTTCGCCCTGTGCTGGTTCCCTCTTCACTTAAGCCGCAT TTTGAAGAAAACTGTATATGATGAGATGGATAAGAACCGGTGTGAACTGCTCAGCTTCTTGCTGCTAATG GATTACATCGGCATTAACCTGGCAACCATGAATTCTTGCATAAACCCAATAGCTCTATATTTTGTGAGCA AGAAATTCAAAAATTGTTTTCAGTCCTGCCTCTGTTGCTGTTGTCACCAGTCCAAAAGCCTCATGACCTC GGTCCCCATGAATGGAACGAGTATCCAGTGGAAGAACCAAGAGCAGAACAACCACAACACGGAACGGAGC AGCCACAAGGACAGCATGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206815 protein sequence
Red=Cloning site Green=Tags(s) MSIFCLAAYFWLTMVGGVMADNPERYSANLSSHMEDFTPFPGTEINFLGTTHRPPNLALPSNGSMHGYCP QQTKITTAFKYINTVISCTIFIVGMVGNATLLRIIYQNKCMRNGPNALIASLALGDLIYVVIDLPINVFK LLAGRWPFDHNDFGVFLCKLFPFLQKSSVGITVLNLCALSVDRYRAVASWSRVQGIGIPLITAIEIVSIW ILSFILAIPEAIGFVMVPFEYKGELHRTCMLNATSKFMEFYQDVKDWWLFGFYFCMPLVCTAIFYTLMTC EMLNRRNGSLRIALSEHLKQRREVAKTVFCLVVIFALCWFPLHLSRILKKTVYDEMDKNRCELLSFLLLM DYIGINLATMNSCINPIALYFVSKKFKNCFQSCLCCCCHQSKSLMTSVPMNGTSIQWKNQEQNNHNTERS SHKDSMN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010332 |
| ORF Size | 1284 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010332.2, NP_034462.1 |
| RefSeq Size | 3643 bp |
| RefSeq ORF | 1284 bp |
| Locus ID | 13617 |
| UniProt ID | Q61614 |
| MW | 48.6 kDa |
| Gene Summary | Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200257 | Ednra (untagged) - Mouse endothelin receptor type A (Ednra), (10ug) |
CNY 5,488.00 |
|
| MG206815 | Ednra (tGFP-tagged) - Mouse endothelin receptor type A (Ednra) |
CNY 7,088.00 |
|
| MR206815L1 | Lenti ORF clone of Ednra (Myc-DDK-tagged) - Mouse endothelin receptor type A (Ednra) |
CNY 7,888.00 |
|
| MR206815L2 | Lenti ORF clone of Ednra (mGFP-tagged) - Mouse endothelin receptor type A (Ednra) |
CNY 6,180.00 |
|
| MR206815L3 | Lenti ORF clone of Ednra (Myc-DDK-tagged) - Mouse endothelin receptor type A (Ednra) |
CNY 6,180.00 |
|
| MR206815L4 | Lenti ORF clone of Ednra (mGFP-tagged) - Mouse endothelin receptor type A (Ednra) |
CNY 6,180.00 |
