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Flot1 (NM_008027) Mouse Tagged ORF Clone
CAT＃: MR206823
- TrueORF®
Flot1 (Myc-DDK-tagged) - Mouse flotillin 1 (Flot1)
ORF Plasmid: tGFP
"NM_008027" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | reggie-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206823 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTCCGGGTTCTGCAGGAGCCCCCCAGTCATGG TGGCCGGAGGCCGAGTGTTTGTCCTACCCTGCATTCAGCAAATCCAGAGGATCTCTCTCAACACACTGAC CCTCAATGTCAAGAGTGAAAAGGTTTATACCCGCCACGGGGTCCCCATCTCAGTCACGGGCATTGCCCAG GTGAAAATCCAGGGCCAGAACAAGGAGATGCTGGCAGCTGCCTGCCAGATGTTCCTGGGGAAGACAGAGG CGGAGATTGCCCACATTGCCCTGGAGACACTGGAAGGCCACCAGAGGGCAATCATGGCCCACATGACTGT GGAGGAGATCTATAAGGATAGACAGAAATTCTCAGAGCAGGTTTTCAAAGTGGCCTCCTCCGACCTGGTC AACATGGGTATCAGCGTGGTTAGCTACACTTTGAAGGATATTCATGATGACCAGGATTACTTACACTCGT TAGGAAAGGCTCGCACGGCACAAGTCCAAAAAGATGCTCGGATTGGCGAAGCAGAGGCCAAGAGAGATGC CGGGATACGGGAGGCTAAGGCCAAGCAGGAGAAGGTATCTGCACAGTGCCTCAGTGAAATAGAGATGGCC AAGGCACAGAGGGACTATGAGCTGAAGAAAGCCACCTACGACATCGAGGTCAACACCCGGCGAGCACAGG CTGACCTGGCCTATCAGCTGCAGGTGGCCAAGACGAAGCAGCAGATCGAGGAGCAGCGGGTGCAGGTGCA GGTGGTTGAGCGCGCCCAGCAGGTGGCAGTGCAGGAGCAGGAGATCGCCCGGCGCGAGAAGGAGTTGGAG GCTCGCGTGCGCAAGCCAGCAGAGGCTGAGCGCTACAGGCTGGAGCGCCTGGCGGAAGCAGAGAAGGCCC AGCTGATCATGCAGGCAGAAGCCGAAGCTGAGTCTGTGCGGATGCGAGGGGAAGCTGAGGCCTTTGCTAT AGGTGCCCGCGCTCGTGCTGAGGCTGAGCAAATGGCCAAGAAGGCAGAAGCCTTCCAGATGTACCAGGAG GCCGCCCAGCTGGACATGCTGCTGGAGAAACTGCCCCAGGTGGCAGAAGAGATCAGTGGTCCCCTGACCT CAGCCAATAAGATCACGCTGGTGTCTAGTGGAAGCGGAACCATGGGGGCAGCCAAAGTTACTGGGGAAGT ACTGGACATCTTGAGCCGCCTGCCGGAGAGTGTGGAGAGGCTCACAGGCGTCAGCATCTCCCAGGTAAAT CACAACAAGCCTTTAAGGACAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206823 protein sequence
Red=Cloning site Green=Tags(s) MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQCLSEIEMA KAQRDYELKKATYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE ARVRKPAEAERYRLERLAEAEKAQLIMQAEAEAESVRMRGEAEAFAIGARARAEAEQMAKKAEAFQMYQE AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILSRLPESVERLTGVSISQVN HNKPLRTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008027 |
| ORF Size | 1287 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008027.1 |
| RefSeq Size | 1694 bp |
| RefSeq ORF | 1287 bp |
| Locus ID | 14251 |
| UniProt ID | O08917 |
| MW | 47.5 kDa |
| Gene Summary | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200016 | Flot1 (untagged) - Mouse flotillin 1 (Flot1), (10ug) |
CNY 5,488.00 |
|
| MG206823 | Flot1 (tGFP-tagged) - Mouse flotillin 1 (Flot1) |
CNY 7,088.00 |
|
| MR206823L3 | Lenti ORF clone of Flot1 (Myc-DDK-tagged) - Mouse flotillin 1 (Flot1) |
CNY 6,180.00 |
|
| MR206823L4 | Lenti ORF clone of Flot1 (mGFP-tagged) - Mouse flotillin 1 (Flot1) |
CNY 6,180.00 |
