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Mlkl (NM_029005) Mouse Tagged ORF Clone
CAT＃: MR207406
- TrueORF®
Mlkl (Myc-DDK-tagged) - Mouse mixed lineage kinase domain-like (Mlkl)
ORF Plasmid: tGFP
"NM_029005" in other vectors (5)
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CNY 3,656.00
CNY 4,090.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9130019I15Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207406 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAATTGGGACAGATCATCAAGTTAGGCCAGCTCATCTATGAACAGTGTGAAAAGATGAAATACT GCCGGAAACAATGCCAGCGTCTAGGAAACCGTGTGCACGGCCTGCTACAGCCTCTCCAGAGACTCCAGGC CCAAGGAAAGAAGAACCTGCCCGATGACATTACTGCTGCCCTGGGCCGTTTTGATGAAGTCCTGAAGGAG GCTAACCAGCAGATAGAAAAGTTCAGCAAGAAGTCCCATATTTGGAAGTTTGTGAGTGTGGGCAATGATA AGATCCTCTTCCATGAAGTGAATGAGAAGCTGAGAGACGTCTGGGAGGAGCTGTTGCTGCTGCTTCAGGT TTATCATTGGAATACCGTTTCAGATGTCAGCCAGCCAGCATCCTGGCAGCAGGAAGATCGACAGGATGCA GAGGAAGACGGAAATGAAAATATGAAAGTTATCCTGATGCAGTTGCAAATTAGCGTGGAAGAAATCAACA AAACCCTGAAGCAATGCTCACTAAAACCCACACAGGAGATCCCACAAGATCTCCAAATCAAGGAGATTCC AAAGGAACATCTTGGACCTCCGTGGACCAAACTGAAGACAAGTAAAATGAGCACCATTTATAGAGGAGAG TATCACAGATCTCCAGTTACCATCAAAGTATTCAACAACCCCCAGGCCGAAAGTGTTGGAATAGTGAGGT TCACTTTCAATGACGAGATCAAAACCATGAAGAAATTCGATTCTCCCAACATCTTGCGTATATTTGGGAT TTGCATTGATCAAACAGTGAAGCCCCCTGAGTTCTCCATTGTCATGGAGTACTGTGAACTTGGAACCCTG AGGGAACTGCTGGATAGAGAAAAAGACCTCACAATGAGTGTGCGCAGCCTCCTAGTCCTGAGGGCAGCCA GAGGCTTATACAGGCTACACCATTCGGAAACACTCCACAGAAACATCAGCAGCTCCAGTTTCCTCGTAGC CGGAGGCTACCAAGTAAAGCTTGCAGGATTTGAGTTAAGCAAAACACAGAATTCCATCAGCCGGACAGCA AAGAGCACTAAAGCAGAGAGATCCAGTTCAACGATATATGTCTCCCCTGAGAGACTGAAAAATCCATTTT GCCTTTATGACATAAAAGCTGAAATATATAGCTTTGGAATTGTACTCTGGGAAATTGCCACTGGAAAGAT CCCATTTGAAGGCTGTGATTCTAAGAAGATCCGGGAGCTGGTGGCTGAGGACAAGAAGCAGGAACCAGTG GGTCAGGATTGCCCTGAGTTGTTGCGGGAAATCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGC CCTCTGTGGACGGAATCTTGGAGAGACTGTCTGCGGTTGAAGAATCCACGGACAAGAAGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207406 protein sequence
Red=Cloning site Green=Tags(s) MDKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALGRFDEVLKE ANQQIEKFSKKSHIWKFVSVGNDKILFHEVNEKLRDVWEELLLLLQVYHWNTVSDVSQPASWQQEDRQDA EEDGNENMKVILMQLQISVEEINKTLKQCSLKPTQEIPQDLQIKEIPKEHLGPPWTKLKTSKMSTIYRGE YHRSPVTIKVFNNPQAESVGIVRFTFNDEIKTMKKFDSPNILRIFGICIDQTVKPPEFSIVMEYCELGTL RELLDREKDLTMSVRSLLVLRAARGLYRLHHSETLHRNISSSSFLVAGGYQVKLAGFELSKTQNSISRTA KSTKAERSSSTIYVSPERLKNPFCLYDIKAEIYSFGIVLWEIATGKIPFEGCDSKKIRELVAEDKKQEPV GQDCPELLREIINECRAHEPSQRPSVDGILERLSAVEESTDKKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029005 |
| ORF Size | 1395 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029005.3 |
| RefSeq Size | 1702 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 74568 |
| UniProt ID | Q9D2Y4 |
| MW | 53.4 kDa |
| Gene Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
,null,
Cell Death and Differentiation
,PubMed ID 34999730
[Mlkl]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207406 | Mlkl (tGFP-tagged) - Mouse mixed lineage kinase domain-like (Mlkl) |
CNY 4,470.00 |
|
| MR207406L1 | Lenti ORF clone of Mlkl (Myc-DDK-tagged) - Mouse mixed lineage kinase domain-like (Mlkl) |
CNY 5,990.00 |
|
| MR207406L2 | Lenti ORF clone of Mlkl (mGFP-tagged) - Mouse mixed lineage kinase domain-like (Mlkl) |
CNY 5,990.00 |
|
| MR207406L3 | Lenti ORF clone of Mlkl (Myc-DDK-tagged) - Mouse mixed lineage kinase domain-like (Mlkl) |
CNY 5,990.00 |
|
| MR207406L4 | Lenti ORF clone of Mlkl (mGFP-tagged) - Mouse mixed lineage kinase domain-like (Mlkl) |
CNY 5,990.00 |
