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Kremen1 (NM_032396) Mouse Tagged ORF Clone
CAT＃: MR207561
- TrueORF®
Kremen1 (Myc-DDK-tagged) - Mouse kringle containing transmembrane protein 1 (Kremen1)
ORF Plasmid: tGFP
"NM_032396" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV002070; Kremen; Krm1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207561 representing NM_032396
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCCCGCCGCCCGTCTCGCGCTGCTCTCCGCCGCTGCGCTCACTCTGGCGGCCCGGCCCGCGC CCGGTCCCCGCTCCGGCCCCGAGTGCTTCACAGCCAACGGTGCAGATTACAGGGGAACACAGAGCTGGAC AGCGCTGCAAGGTGGGAAGCCATGTCTGTTCTGGAACGAGACTTTCCAGCATCCGTACAACACGCTGAAG TACCCCAACGGGGAAGGAGGACTGGGCGAGCACAATTATTGCAGAAATCCAGATGGAGACGTGAGCCCTT GGTGCTACGTGGCCGAGCATGAGGACGGAGTCTACTGGAAGTACTGTGAAATTCCTGCCTGCCAGATGCC TGGAAACCTTGGCTGCTACAAGGATCATGGAAACCCACCTCCTCTCACGGGCACCAGTAAAACCTCTAAC AAGCTCACCATACAAACCTGTATCAGCTTCTGTCGGAGTCAGAGATTCAAGTTTGCTGGGATGGAGTCAG GCTATGCCTGCTTCTGTGGGAACAATCCTGACTACTGGAAGCACGGGGAGGCGGCCAGCACCGAGTGCAA TAGTGTCTGCTTCGGGGACCACACGCAGCCCTGCGGTGGGGACGGCAGGATTATCCTCTTTGACACTCTC GTGGGCGCCTGCGGTGGGAACTACTCAGCCATGGCAGCCGTGGTGTACTCCCCTGACTTCCCTGACACCT ACGCCACTGGCAGAGTCTGCTACTGGACCATCCGGGTTCCAGGAGCCTCTCGCATCCATTTCAACTTCAC CCTGTTTGATATCAGGGACTCTGCAGACATGGTGGAGCTGCTGGACGGCTACACCCACCGCGTCCTGGTC CGGCTCAGTGGGAGGAGCCGCCCGCCTCTGTCTTTCAATGTCTCTCTGGATTTTGTCATTTTGTATTTCT TCTCTGATCGCATCAATCAGGCCCAGGGATTTGCTGTGTTGTACCAAGCCACCAAGGAGGAACCGCCACA GGAGAGACCTGCTGTCAACCAGACCCTGGCAGAGGTGATCACCGAGCAAGCCAACCTCAGTGTCAGCGCT GCCCACTCCTCCAAAGTCCTCTATGTCATCACCCCCAGCCCCAGCCACCCTCCGCAGACTGCCCCAGGTA GCCATTCCTGGGCACCGTCAGTTGGGGCCAACAGCCACAGAGTGGAAGGATGGACTGTGTACGGCCTGGC GACCCTCCTCATCCTCACAGTCACAGCAGTTGTCGCAAAGATTCTTCTGCATGTCACATTTAAATCTCAT CGAGTCCCTGCATCAGGAGACCTTAGGGACTGTCGTCAGCCTGGGGCTTCTGGAGATATCTGGACCATTT TCTATGAACCTTCCACTACAATCTCCATCTTTAAGAAGAAGCTCAAGGGTCAGAGTCAACAAGATGACCG CAATCCCCTCGTGAGTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207561 representing NM_032396
Red=Cloning site Green=Tags(s) MAPPAARLALLSAAALTLAARPAPGPRSGPECFTANGADYRGTQSWTALQGGKPCLFWNETFQHPYNTLK YPNGEGGLGEHNYCRNPDGDVSPWCYVAEHEDGVYWKYCEIPACQMPGNLGCYKDHGNPPPLTGTSKTSN KLTIQTCISFCRSQRFKFAGMESGYACFCGNNPDYWKHGEAASTECNSVCFGDHTQPCGGDGRIILFDTL VGACGGNYSAMAAVVYSPDFPDTYATGRVCYWTIRVPGASRIHFNFTLFDIRDSADMVELLDGYTHRVLV RLSGRSRPPLSFNVSLDFVILYFFSDRINQAQGFAVLYQATKEEPPQERPAVNQTLAEVITEQANLSVSA AHSSKVLYVITPSPSHPPQTAPGSHSWAPSVGANSHRVEGWTVYGLATLLILTVTAVVAKILLHVTFKSH RVPASGDLRDCRQPGASGDIWTIFYEPSTTISIFKKKLKGQSQQDDRNPLVSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032396 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032396.3, NP_115772.2 |
| RefSeq Size | 4943 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 84035 |
| UniProt ID | Q99N43 |
| MW | 52.1 kDa |
| Gene Summary | Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6 (PubMed:12050670). In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane (By similarity). Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1 (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation (PubMed:18505822). Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification (PubMed:27550540).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216539 | Kremen1 (untagged) - Mouse kringle containing transmembrane protein 1 (Kremen1), (10ug) |
CNY 3,656.00 |
|
| MG207561 | Kremen1 (tGFP-tagged) - Mouse kringle containing transmembrane protein 1 (Kremen1) |
CNY 3,710.00 |
|
| MR207561L3 | Lenti ORF clone of Kremen1 (Myc-DDK-tagged) - Mouse kringle containing transmembrane protein 1 (Kremen1) |
CNY 5,230.00 |
|
| MR207561L4 | Lenti ORF clone of Kremen1 (mGFP-tagged) - Mouse kringle containing transmembrane protein 1 (Kremen1) |
CNY 5,230.00 |
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