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Sesn2 (NM_144907) Mouse Tagged ORF Clone
CAT＃: MR207693
- TrueORF®
Sesn2 (Myc-DDK-tagged) - Mouse sestrin 2 (Sesn2)
ORF Plasmid: tGFP
"NM_144907" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HI95; Ses2; SEST2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207693 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGTAGCGGACTCCGAGTGCCATTCCGAGATCAAGGGCTACCTGCCGTTCACCAGGGGTGGTGTGG CCGGCCCCGAGACCCGAGAGGAGCATCGGGAGGGCCAGGCTCGGCGAGGCTCTCGAGGGCCCAGCGCTTT CATTCCAGTGGAAGAGATCCTTCGGGAAGGAGCTGAGAGCCTGGAGCAGCACCTGGGGCTCGAGGCCCTG ATGTCCTCAGGGCGGGTGGACAACCTGGCGGTGGTGATGGGTCTACACCCGGACTACCTTAGCAGCTTCT GGCGCCTGCATTACCTGCTGCTGCATACGGATGGGCCCCTGGCCAGCTCCTGGCGCCACTACATTGCCAT CATGGCTGCTGCCCGTCACCAGTGTTCTTACCTGGTGGGTTCCCACATGACTGAGTTCCTGCAGACCGGC GGTGACCCCGAGTGGCTGCTGGGCCTTCACCGGGCCCCTGAGAAGCTCCGCAAGCTCAGCGAGGTCAACA AGTTACTGGCGCATCGGCCGTGGCTCATCACCAAGGAGCATATCCAGGCCTTGCTGAAGACTGGCGAGCA CAGCTGGTCCCTGGCCGAACTCATCCAAGCACTGGTCCTGCTTACCCACTGCCACTCTCTGGCCTCCTTT GTGTTTGGCTGTGGAATACTTCCTGAGGGAGATGCAGAGGGCAGCCCTGCCTCCCAGGCACCTTCGCCTC CCAGTGAGCAAGGCACCCCCCCGAGCGGGGACCCACTGAACAACTCAGGGGGCTTTGAAGCCGCTCGTGA CGTAGAAGCTCTGATGGAACGCATGAGGCAGCTTCAGGAGAGCCTGCTGCGGGATGAGGGGGCTTCGCAG GAGGAAATGGAGAACCGTTTTGAGCTGGAGAAGTCAGAAAGCCTGCTGGTGACCCCCTCAGCGGATATCC TGGAGCCTTCTCCACACCCAGACATCCTATGCTTTGTGGAGGACCCCGCATTTGGATATGAAGACTTCAC TCGTCGAGGGACTCAGGCGCCCCCAACCTTCCGTGCCCAGGATTATACCTGGGAAGACCATGGCTACTCG CTGATCCAGAGGCTCTACCCTGAGGGAGGGCAGCTGCTGGATGAGAAGTTCCAGGTAGCCTGCAGCCTCA CCTATAACACCATCGCCATGCACAGTGGCGTGGACACCTCCATGCTCCGCAGGGCCATCTGGAACTACAT CCACTGCGTCTTTGGCATCAGATACGATGACTATGATTACGGCGAGGTAAACCAGCTCCTGGAACGGAAC CTCAAAATCTATATCAAGACGGTGGCCTGCTACCCTGAGAAGACGACCCGTAGGATGTACAACCTCTTTT GGAGGCACTTCCGCCACTCAGAGAAGGTTCATGTGAACTTGCTGCTCCTTGAAGCCCGCATGCAAGCGGC CCTGCTCTATGCCCTGCGCGCCATCACCCGCTACATGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207693 protein sequence
Red=Cloning site Green=Tags(s) MIVADSECHSEIKGYLPFTRGGVAGPETREEHREGQARRGSRGPSAFIPVEEILREGAESLEQHLGLEAL MSSGRVDNLAVVMGLHPDYLSSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMTEFLQTG GDPEWLLGLHRAPEKLRKLSEVNKLLAHRPWLITKEHIQALLKTGEHSWSLAELIQALVLLTHCHSLASF VFGCGILPEGDAEGSPASQAPSPPSEQGTPPSGDPLNNSGGFEAARDVEALMERMRQLQESLLRDEGASQ EEMENRFELEKSESLLVTPSADILEPSPHPDILCFVEDPAFGYEDFTRRGTQAPPTFRAQDYTWEDHGYS LIQRLYPEGGQLLDEKFQVACSLTYNTIAMHSGVDTSMLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERN LKIYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144907 |
| ORF Size | 1443 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144907.1, NP_659156.1 |
| RefSeq Size | 2677 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 230784 |
| UniProt ID | P58043 |
| MW | 54.4 kDa |
| Gene Summary | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:18692468, PubMed:25259925). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (By similarity). However, this could not be confirmed (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200569 | Sesn2 (untagged) - Mouse sestrin 2 (Sesn2), (10ug) |
CNY 2,000.00 |
|
| MG207693 | Sesn2 (tGFP-tagged) - Mouse sestrin 2 (Sesn2) |
CNY 4,750.00 |
|
| MR207693L3 | Lenti ORF clone of Sesn2 (Myc-DDK-tagged) - Mouse sestrin 2 (Sesn2) |
CNY 6,180.00 |
|
| MR207693L4 | Lenti ORF clone of Sesn2 (mGFP-tagged) - Mouse sestrin 2 (Sesn2) |
CNY 6,180.00 |
