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Hcls1 (NM_008225) Mouse Tagged ORF Clone
CAT＃: MR207789
- TrueORF®
Hcls1 (Myc-DDK-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1)
ORF Plasmid: tGFP
"NM_008225" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW213261; HS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207789 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAGTCTGTAGTGGGGCATGATGTATCGGTTTCCGTGGAGACCCAGGGTGATGACTGGGATACAG ACCCTGACTTTGTGAATGACATCTCCGAGAAGGAGCAACGGTGGGGAGCCAAGACCATTGAGGGCTCTGG ACGCACAGAGCACATCAACATCCACCAGCTGAGGAACAAAGTGTCAGAGGAGCACGACATCCTCAAGAAG AAGGAGCTGGAATCGGGGCCTAAGGCATCCCATGGCTATGGCGGTCGGTTTGGAGTGGAGAGAGACCGGA TGGACAAGAGTGCCGTGGGCCACGAGTATGTTGCTGATGTGGAGAAACACTCATCTCAGACTGATGCCGC CAGAGGCTTTGGGGGCAAATATGGAGTTGAGAGGGACCGGGCAGACAAGTCAGCGGTGGGCTTTGACTAC AAAGGAGAAGTGGAAAAGCATGCATCTCAGAAAGATTACTCTCATGGCTTTGGTGGCCGCTACGGGGTAG AGAAGGATAAACGGGACAAAGCAGCCCTGGGATACGACTACAAAGGAGAGACGGAGAAGCACGAGTCTCA GAGAGATTATGCCAAGGGCTTTGGTGGCCAATATGGAATCCAGAAAGACCGAGTGGATAAGAGTGCTGTT GGCTTCAATGAAATGGAGGCCCCAACCACGGCGTATAAGAAGACAACACCCATAGAAGCTGCTTCCAGTG GTGCCCGTGGGCTGAAGGCAAAATTTGAGTCCCTGGCTGAGGAGAAGAGGAAGCGAGAGGAAGAAGAGAA GGCACAGCAGATGGCCAGGCAGCAACAGGAGCGAAAGGCTGTGGTAAAGATGAGCCGAGAAGTCCAGCAG CCATCCATGCCTGTGGAAGAGCCAGCGGCACCAGCCCAGTTGCCCAAGAAGATCTCCTCAGAGGTCTGGC CTCCAGCAGAGAGTCACCTACCGCCAGAGTCTCAGCCAGTGAGAAGCAGAAGGGAATACCCTGTGCCCTC TCTGCCCACGAGGCAGTCTCCATTGCAGAATCACTTGGAGGACAACGAGGAGCCCCCAGCTCTGCCCCCT AGGACCCCAGAAGGCCTCCAGGTGGTGGAAGAGCCAGTGTACGAAGCAGCACCCGAGCTGGAGCCGGAGC CAGAGCCTGACTATGAGCCAGAGCCAGAGACAGAGCCTGACTATGAGGATGTTGGGGAGTTAGATCGGCA GGATGAGGATGCAGAGGGAGACTATGAGGATGTGCTGGAGCCCGAGGACACCCCTTCTCTGTCCTACCAA GCTGGACCCTCAGCTGGGGCTGGTGGTGCGGGGATCTCTGCTATAGCCCTGTATGATTACCAAGGAGAGG GAAGCGATGAGCTTTCCTTTGATCCAGATGACATCATCACTGACATTGAGATGGTGGATGAAGGCTGGTG GCGGGGCCAATGCCGTGGCCACTTTGGACTTTTCCCTGCAAACTATGTCAAGCTCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207789 protein sequence
Red=Cloning site Green=Tags(s) MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKK KELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDY KGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAV GFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQ PSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPP RTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQ AGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008225 |
| ORF Size | 1461 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008225.2, NP_032251.2 |
| RefSeq Size | 2031 bp |
| RefSeq ORF | 1461 bp |
| Locus ID | 15163 |
| UniProt ID | P49710 |
| MW | 54.2 kDa |
| Gene Summary | Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216597 | Hcls1 (untagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1), (10ug) |
CNY 6,270.00 |
|
| MG207789 | Hcls1 (tGFP-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1) |
CNY 3,710.00 |
|
| MR207789L3 | Lenti ORF clone of Hcls1 (Myc-DDK-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1) |
CNY 5,230.00 |
|
| MR207789L4 | Lenti ORF clone of Hcls1 (mGFP-tagged) - Mouse hematopoietic cell specific Lyn substrate 1 (Hcls1) |
CNY 5,230.00 |
