Oma1 (NM_025909) Mouse Tagged ORF Clone
CAT#: MR208350
- TrueORF®
Oma1 (Myc-DDK-tagged) - Mouse OMA1 homolog, zinc metallopeptidase (S. cerevisiae) (Oma1)
ORF Plasmid: tGFP
"NM_025909" in other vectors (4)
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CNY 5,832.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2010001O09Rik; MPRP-1; ZMPOMA1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208350 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTCCTTTATGGACTGCAGTCTACCAGGATAAATCGGTTTCTCTCTGGAGTGAATAACCTGGCCA ACAGGAGACAGTGGACCCCCCCAGCAAGCTGTCCACTGGCACCAAAGCTCCGAGCAGTAAATGCATACTG GGGACTGAACACAGTCAGTCATTGTCATTCAGTGACCTTACTGCCTAGAAACTTTCTTTTCTGTAGGACT CTCAATCACAAAAAATCAAGATGCCTCTCAAGTGCCCAAAGCAAGGAATTGGGGGTGCTTACCTACAGAT GTACTGTGCGGGGTGATTCTGTTCTAAGACAAGGAGCAAGGAAAGTGGCTGGCGTTCCTGCTCTCGCGGC CTCCTGTTCTCCAAGCTGTCCTGCCGTAATAGAGGCCCGGAGTTTCCGTACATCTGCAAGGGTTCAGGCT GCCCCAGTCCCTCTCTTGCTGCTCATTCTGAAGCCAGTGCAAAAGCTCCTTGCTATCATCGTGGGCAGGG GCATAAGGAAATGGTGGCAAGCACTTCCCCCTAACAAGAAGGAGCTATTTAAAGACAGCGTGAGGAAGAA CAAGTGGCGGCTGCTTCTTGGTCTGAGTGCATTTGGACTGCTCTTTGTAGTGTTTTATTTCACTCACCTG GAAGTGAGCCCTGTCACCGGAAGGAGTAAACTACTGTTAGTGGGCAAAGAACACTTCCGACTTCTGTCAG ACCTGGAATATGAAGTATGGATGGAAGAATTTAAGAACGATCTGCTGCCTGAGAGAGACCCCCGCTACCT GACTGTGAAAGAAATGGTTTATCATCTGACACAGTGCAATCGAGACGTCCCGGGGATCTCGGAGACCAAC TGGGTGGTTCATGTGGTTGACTCCCCAGCCGTCAATGCCTTTGTGCTCCCAAATGGACAAGTGTTTATTT TCACCGGGCTTCTGAATAGTGTGACGGACGTGCACCAGCTGTCCTTCCTCCTGGGCCATGAGATCGCACA CGCAGTCCTGGGGCACGCCGCAGAAAAGGCTAGCTTGGTTCATTTACTGGATTTCCTTGGTATGATTTTT CTCACAATGATTTGGGCCATTTGTCCCCGAGACAGCTTGGCAGTTTTGGGCCAGTGGATACAGTCAAAGT TGCAGGAGTACATGTTTGATCGACCATACAGTAGAACATTGGAGGCCGAAGCTGACAAAGTTGGACTACA GCTGGCTGCAAAGGCATGTGCCGATGTAAGAGCTAGTTCAGTGTTTTGGCAGCAGATGGAGTTCTCAGAG AGTCTTCATGGCTATCCCAAGTTGCCAGAGTGGCTGTCGACACATCCTTCTCATGGGAACCGAGCTGAGT ACTTGGATAGACTCATACCACAGGCTCTCAAACTCCGGGAGGTCTGCAATTGCCCGCCACTCTCTGGCCC AGACCCTCGGCTATTGTTCAGGCTTACTGTGAAGCGTTTTCTTGAAGACTCGGAGAAAGAGGACCTAAAC ATCACTGTCAAAAAGCAGAAAACGGATGCTCTCCCCATGCAGAAGCAGGAGCAGATACCACTGACGTATG TCCTAGAGAAGAGAACTGCAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208350 protein sequence
Red=Cloning site Green=Tags(s) MSLLYGLQSTRINRFLSGVNNLANRRQWTPPASCPLAPKLRAVNAYWGLNTVSHCHSVTLLPRNFLFCRT LNHKKSRCLSSAQSKELGVLTYRCTVRGDSVLRQGARKVAGVPALAASCSPSCPAVIEARSFRTSARVQA APVPLLLLILKPVQKLLAIIVGRGIRKWWQALPPNKKELFKDSVRKNKWRLLLGLSAFGLLFVVFYFTHL EVSPVTGRSKLLLVGKEHFRLLSDLEYEVWMEEFKNDLLPERDPRYLTVKEMVYHLTQCNRDVPGISETN WVVHVVDSPAVNAFVLPNGQVFIFTGLLNSVTDVHQLSFLLGHEIAHAVLGHAAEKASLVHLLDFLGMIF LTMIWAICPRDSLAVLGQWIQSKLQEYMFDRPYSRTLEAEADKVGLQLAAKACADVRASSVFWQQMEFSE SLHGYPKLPEWLSTHPSHGNRAEYLDRLIPQALKLREVCNCPPLSGPDPRLLFRLTVKRFLEDSEKEDLN ITVKKQKTDALPMQKQEQIPLTYVLEKRTAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025909 |
ORF Size | 1566 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025909.3 |
RefSeq Size | 1866 bp |
RefSeq ORF | 1566 bp |
Locus ID | 67013 |
UniProt ID | Q9D8H7 |
MW | 58.9 kDa |
Gene Summary | Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion (PubMed:22433842, PubMed:24616225, PubMed:26785494). May also cleave UQCC3 under these conditions. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Prohibitin levels regulate OMA1 activity and turnover in neurons
,Anderson, CJ;Kahl, A;Fruitman, H;Qian, L;Zhou, P;Manfredi, G;Iadecola, C;,
Cell Death Differ.
,PubMed ID 31819158
[OMA1]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205339 | Oma1 (untagged) - Mouse OMA1 homolog, zinc metallopeptidase (S. cerevisiae) (Oma1), (10ug) |
CNY 2,000.00 |
|
MG208350 | Oma1 (tGFP-tagged) - Mouse OMA1 homolog, zinc metallopeptidase (S. cerevisiae) (Oma1) |
CNY 7,432.00 |
|
MR208350L3 | Lenti ORF clone of Oma1 (Myc-DDK-tagged) - Mouse OMA1 homolog, zinc metallopeptidase (S. cerevisiae) (Oma1) |
CNY 6,460.00 |
|
MR208350L4 | Lenti ORF clone of Oma1 (mGFP-tagged) - Mouse OMA1 homolog, zinc metallopeptidase (S. cerevisiae) (Oma1) |
CNY 6,460.00 |