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Yars (NM_134151) Mouse Tagged ORF Clone
CAT＃: MR208462
- TrueORF®
Yars (Myc-DDK-tagged) - Mouse tyrosyl-tRNA synthetase (Yars)
ORF Plasmid: tGFP
"NM_134151" in other vectors (4)
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CNY 3,936.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL024047; AU018965 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208462 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGATGCTCCAAGCCCTGAGGAGAAGCTGCATCTTATCACCCGGAACCTGCAGGAGGTTCTGGGGG AAGAGAAGCTGAAGGAGATCCTGAAGGAGCGGGAACTTAAAGTTTACTGGGGCACGGCCACCACGGGCAA GCCACACGTGGCTTACTTTGTACCCATGTCCAAGATCGCTGACTTCTTGAAGGCAGGGTGTGAGGTAACC ATCCTGTTTGCGGACCTCCATGCATACCTGGACAACATGAAAGCCCCCTGGGAGCTTCTAGAACTTCGAA CCAGTTACTATGAGAATGTGATCAAGGCCATGCTGGAGAGTATTGGCGTGCCCTTGGAGAAGCTCAAGTT TATCAAAGGCACCGACTACCAGCTCAGCAAAGAGTACACACTGGATGTGTACCGACTGTCCTCTGTGGTC ACACAACATGACGCCAAGAAAGCGGGGGCTGAGGTTGTGAAGCAGGTGGAACACCCTTTGCTGAGTGGTC TGCTGTACCCCGGTCTGCAGGCTTTGGATGAAGAGTACTTAAAAGTAGACGCCCAGTTCGGCGGTGTTGA TCAGAGGAAGATTTTTACCTTTGCAGAGAAGTATCTTCCTGCACTTGGCTACTCAAAACGGGTCCACCTG ATGAATCCCATGGTCCCTGGGTTAACTGGGAGCAAAATGAGCTCGTCAGAAGAGGAGTCCAAAATCGACC TCCTTGATCGGAAGGAGGACGTGAAGAAAAAGCTGAAGAAGGCCTTCTGTGAGCCCGGCAATGTGGAGAA CAACGGGGTTCTGTCCTTCATCAAGCATGTCCTCTTTCCCCTCAAGTCTGAGTTTGTGATCCTGAGAGAT GAGAAGTGGGGTGGAAACAAGACCTACACCGTTTACCTAGAGCTGGAGAAGGACTTCGCTGCTGAGGTCG TACACCCTGGAGACCTAAAGAATTCTGTTGAAGTCGCCCTGAACAAGTTGCTGGACCCCATTAGAGAGAA GTTTAATACTCCAGCCCTGAAGAAGCTGGCCAGTGCTGCCTACCCAGATCCTTCAAAGCAGAAGCCTCCT GCCAAAGGCCCTGCCAAGAACTCAGAACCAGAGGAAGTCATCCCATCCCGGCTGGATATCCGTGTGGGCA AAATCCTCAGCGTGGAGAAGCACCCAGATGCAGATAGCCTTTATGTGGAGAAGATTGATGTGGGGGAAGC TGAACCCAGGACTGTGGTGAGCGGCCTCGTACAGTTTGTGCCCAAAGAAGAACTGCAGGACAGGCTGGTG GTGGTGCTGTGCAATCTGAAACCCCAGAAGATGAGAGGCGTGGACTCGCAGGGCATGCTACTGTGTGCTT CTGTAGAAGGGGTGAGCCGCCAGGTTGAACCTCTGGACCCTCCTGCTGGCTCTGCTCCTGGTGAACGAGT GTTCGTACAGGGCTATGAGAAGGGCCAGCCAGATGAGGAGCTCAAGCCCAAGAAGAAAGTCTTTGAGAAG CTGCAGGCCGACTTTAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAACCAACTTCATGACCAAGC TGGGATTCGTCTCCTGTAAATCACTAAAAGGGGGTAACATCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208462 protein sequence
Red=Cloning site Green=Tags(s) MGDAPSPEEKLHLITRNLQEVLGEEKLKEILKERELKVYWGTATTGKPHVAYFVPMSKIADFLKAGCEVT ILFADLHAYLDNMKAPWELLELRTSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVV TQHDAKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGVDQRKIFTFAEKYLPALGYSKRVHL MNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRD EKWGGNKTYTVYLELEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPP AKGPAKNSEPEEVIPSRLDIRVGKILSVEKHPDADSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLV VVLCNLKPQKMRGVDSQGMLLCASVEGVSRQVEPLDPPAGSAPGERVFVQGYEKGQPDEELKPKKKVFEK LQADFKISEECIAQWKQTNFMTKLGFVSCKSLKGGNIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134151 |
| ORF Size | 1587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134151.1 |
| RefSeq Size | 2874 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 107271 |
| UniProt ID | Q91WQ3 |
| MW | 59.1 kDa |
| Gene Summary | Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202144 | Yars (untagged) - Mouse tyrosyl-tRNA synthetase (Yars), (10ug) |
CNY 2,000.00 |
|
| MG208462 | Yars (tGFP-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) |
CNY 5,040.00 |
|
| MR208462L3 | Lenti ORF clone of Yars (Myc-DDK-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) |
CNY 6,460.00 |
|
| MR208462L4 | Lenti ORF clone of Yars (mGFP-tagged) - Mouse tyrosyl-tRNA synthetase (Yars) |
CNY 6,460.00 |
