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Ehd3 (NM_020578) Mouse Tagged ORF Clone
CAT＃: MR208579
- TrueORF®
Ehd3 (Myc-DDK-tagged) - Mouse EH-domain containing 3 (Ehd3)
ORF Plasmid: tGFP
"NM_020578" in other vectors (4)
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CNY 3,992.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ehd2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208579 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGTTGGCTGGGTAACGATGATCGCCGCAAGAAGGACCCTGAGGTCTTCCAGACGGTGAGCGATG GACTCAAAAAACTCTACAAGACCAAGCTGCTGCCTCTGGAAGAGTATTACCGCTTCCACGAGTTCCACTC GCCCGCCCTGGAAGATGCTGATTTCGACAACAAGCCCATGGTCCTGTTGGTGGGCCAGTACTCTACCGGC AAGACCACCTTCATCAGGTACCTGCTGGAACAGGATTTTCCAGGCATGAGGATTGGGCCTGAGCCGACCA CTGATTCCTTCATAGCAGTGATGCAGGGAGATGTGGAGGGGATCATTCCCGGGAATGCCTTGGTGGTGGA TCCAAAGAAACCCTTCAGAAAGCTCAACGCCTTTGGCAACGCCTTCCTGAACAGGTTTGTGTGTGCCCAG CTGCCCAACGCCGTGCTTGAAAGTATCAGTGTGATCGACACACCGGGGATCCTCTCTGGGGAGAAGCAGA GGATCAGCCGAGGGTATGATTTTGCTGCGGTCCTCGAATGGTTTGCTGAGCGGGTGGACCGAATTATCCT ACTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTCTCAGAAGTCATCAAGGCTCTCAAGAACCAT GAGGACAAGATGCGCGTAGTGTTGAACAAAGCTGACCAGATCGAGACCCAGCAGCTGATGCGAGTATACG GAGCCCTCATGTGGTCCCTGGGGAAGATCGTGAACACCCCCGAGGTGATCCGGGTCTACATTGGCTCCTT CTGGTCCCACCCACTCCTCATTCCCGACAACCGGAAGCTCTTTGAAGCTGAAGAGCAAGACTTGTTCAGA GACATTCAGAGTCTACCCCGTAATGCTGCTCTTCGAAAGCTCAACGATCTCATCAAGAGAGCCCGGCTGG CCAAGGTCCACGCCTACATCATCAGCTCCTTGAAGAAGGAGATGCCCTCAGTGTTTGGGAAGGACACCAA AAAGAAAGAACTGGTGAACAACCTGGCTGAGATCTATGGCCGGATTGAGCGAGAACACCAGATCTCCCCT GGAGACTTCCCCAACCTGAAGAGGATGCAGGACCAGCTGCAGGCCCAGGACTTCAGCAAATTCCAGCCAC TGAAGAGCAAGCTGCTGGAAGTGGTTGATGATATGCTGGCTCATGACATTGCCCAGCTCATGGTGCTGGT GCGCCAGGAAGAGACCCAACGGCCTGTCCAGATGGTGAAGGGCGGAGCATTTGAGGGAACCTTACAAGGC CCCTTCGGGCACGGCTATGGAGAGGGAGCTGGGGAGGGCATCGATGATGCCGAGTGGGTGGTGGCGCGGG ACAAGCCTATGTATGATGAGATCTTCTACACCTTATCCCCAGTGGATGGCAAGATCACAGGTGCCAACGC CAAGAAGGAGATGGTGCGCTCCAAGTTGCCCAACAGCGTGCTGGGCAAGATCTGGAAGCTAGCCGACATT GACAAGGATGGCATGTTGGATGACGAGGAGTTTGCCCTGGCCAACCACCTTATCAAAGTCAAGCTAGAGG GGCATGAGCTGCCCAGTGAGCTACCTGCCCACCTCCTCCCTCCATCTAAGAGGAAAGTATCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208579 protein sequence
Red=Cloning site Green=Tags(s) MFSWLGNDDRRKKDPEVFQTVSDGLKKLYKTKLLPLEEYYRFHEFHSPALEDADFDNKPMVLLVGQYSTG KTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDVEGIIPGNALVVDPKKPFRKLNAFGNAFLNRFVCAQ LPNAVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNH EDKMRVVLNKADQIETQQLMRVYGALMWSLGKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFR DIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPSVFGKDTKKKELVNNLAEIYGRIEREHQISP GDFPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEETQRPVQMVKGGAFEGTLQG PFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSVLGKIWKLADI DKDGMLDDEEFALANHLIKVKLEGHELPSELPAHLLPPSKRKVSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020578 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020578.3 |
| RefSeq Size | 3658 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 57440 |
| UniProt ID | Q9QXY6 |
| MW | 60.8 kDa |
| Gene Summary | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes (By similarity). Binding to phosphatidic acid induces its membrane tubulation activity (PubMed:26896729). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology (By similarity). Involved in the recycling of internalized D1 dopamine receptor (By similarity). Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (PubMed:20489164, PubMed:24759929, PubMed:25825486). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (PubMed:21408024). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with Ehd1 (PubMed:25686250).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205480 | Ehd3 (untagged) - Mouse EH-domain containing 3 (Ehd3), (10ug) |
CNY 2,000.00 |
|
| MG208579 | Ehd3 (tGFP-tagged) - Mouse EH-domain containing 3 (Ehd3) |
CNY 5,040.00 |
|
| MR208579L3 | Lenti ORF clone of Ehd3 (Myc-DDK-tagged) - Mouse EH-domain containing 3 (Ehd3) |
CNY 6,460.00 |
|
| MR208579L4 | Lenti ORF clone of Ehd3 (mGFP-tagged) - Mouse EH-domain containing 3 (Ehd3) |
CNY 6,460.00 |
