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Trip10 (NM_134125) Mouse Tagged ORF Clone
CAT＃: MR208703
- TrueORF®
Trip10 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 10 (Trip10)
ORF Plasmid: tGFP
"NM_134125" in other vectors (4)
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CNY 4,080.00
CNY 4,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI646975; Cip4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208703 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGGGGTACCGAGTTGTGGGATCAGTTTGAGGTGCTGGAACGCCACACGCAGTGGGGGCTGGATT TGTTGGACAAATACGTGAAGTTCGTGAAAGAACGCGCGGAGGTGGAGCAGGCTTATGCTAAGCAACTCCG GAGCCTGGTGAAAAAGTATCTTCCCAAGAGACCTACCAAAGATGACCCTGAAGTCAAGTTCAGCCAGCAG CAGTCATTTGTCCAGCTTCTCCAGGAGGTCAATGACTTCGCAGGCCAGAGAGAGCTGGTGGCTGAGAGCC TCGGTATCCGAGTGTGTCTGGAGCTGGCTAAGTATTCACAGGAGATGAAGCAGGAGAGGAAGATGCACTT CCAGGAAGGTCGTCGGGCCCAGCAGCAGCTGGAGAATGGCTTCAAACAGCTGGAGAATAGTAAGCGGAAG TTTGAACGAGATTGCCGGGAGGCTGAGAAAGCGGCTCACACTGCTGAGCGCCTAGATCAGGACATTAATG CCACCAAGGCGGATGTGGAGAAGGCCAAGCAGCAAGCTCACCTTCGGAACCACATGGCAGAAGAGAGCAA GAATGAATATGCGGCCCAGCTGCAGCGCTTCAACCGAGACCAGGCTCACTTCTACTTCTCACAGATGCCC CAGATATTCGATAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCT TATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGA GTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGG GACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGG ATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAA CAAGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAG CTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAGGGAGGCCCTGAAGAAGATGAAAGATG TATATGAGAAAACACCACAAATGGGGGACCCTGCCAGCTTAGAGCCCCGCATTGCAGAGACCCTGGGCAA CATTGAGAGGCTGAAGTTGGAAGTGCAGAAGTATGAGGCTTGGTTGGCAGAAGCTGAAAGCCGGGTCCTC AGTAACCGAGGGGACAGCCTAAGCCGTCACGCTAGGCCCCCTGATCCCCCAACTACTGCCCCACCTGATA GCAGCAGTAGCAGCACCAACAGTGGATCCCAGGACAATAAGGAGAGCAGCTCAGAAGAGCCCCCTTCAGA AGGCCAGGACACCCCCATCTATACTGAGTTCGATGAGGACTTTGAGGAGCCTGCATCCCCTATCGGCCAG TGTGTGGCTATCTACCATTTTGAAGGATCCAGCGAGGGAACCGTCTCCATGTCCGAGGGGGAAGACCTCA GCCTGATGGAGGAAGACAAGGGTGATGGATGGACGCGGGTCAGGAGGAAACAGGGAGCTGAGGGCTACGT GCCCACCTCTTACCTCCGAGTCACACTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208703 protein sequence
Red=Cloning site Green=Tags(s) MDWGTELWDQFEVLERHTQWGLDLLDKYVKFVKERAEVEQAYAKQLRSLVKKYLPKRPTKDDPEVKFSQQ QSFVQLLQEVNDFAGQRELVAESLGIRVCLELAKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRK FERDCREAEKAAHTAERLDQDINATKADVEKAKQQAHLRNHMAEESKNEYAAQLQRFNRDQAHFYFSQMP QIFDKLQDMDERRATRLGAGYGLLSEAELQVVPIIGKCLEGMKVAAESVDAKNDSQVLIELHKSGFARPG DLEFEDFSQVINRVPSDSSLGTPDGRPELRAASSRSRAKRWPFGKKNKTVATEDFSHLPPEQQRKRLQQQ LEERNRELQKEEDQREALKKMKDVYEKTPQMGDPASLEPRIAETLGNIERLKLEVQKYEAWLAEAESRVL SNRGDSLSRHARPPDPPTTAPPDSSSSSTNSGSQDNKESSSEEPPSEGQDTPIYTEFDEDFEEPASPIGQ CVAIYHFEGSSEGTVSMSEGEDLSLMEEDKGDGWTRVRRKQGAEGYVPTSYLRVTLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134125 |
| ORF Size | 1644 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134125.4 |
| RefSeq Size | 2290 bp |
| RefSeq ORF | 1644 bp |
| Locus ID | 106628 |
| UniProt ID | Q8CJ53 |
| MW | 62.7 kDa |
| Gene Summary | Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL (By similarity). Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200148 | Trip10 (untagged) - Mouse thyroid hormone receptor interactor 10 (Trip10), (10ug) |
CNY 2,000.00 |
|
| MG208703 | Trip10 (tGFP-tagged) - Mouse thyroid hormone receptor interactor 10 (Trip10) |
CNY 5,230.00 |
|
| MR208703L3 | Lenti ORF clone of Trip10 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 10 (Trip10) |
CNY 6,650.00 |
|
| MR208703L4 | Lenti ORF clone of Trip10 (mGFP-tagged) - Mouse thyroid hormone receptor interactor 10 (Trip10) |
CNY 6,650.00 |
