Rtl3 (NM_199468) Mouse Tagged ORF Clone
CAT#: MR208781
- TrueORF®
Zcchc5 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 5 (Zcchc5)
ORF Plasmid: tGFP
"NM_199468" in other vectors (4)
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CNY 6,176.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D430021I08Rik; Gm375; Mar3; Mart3; ZHC5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208781 representing NM_199468
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGAGGACTTAGCAGCTTCCTATGTTACTCTGAAATTGGAGAATGAAATTCTGCAGGCTCAAGTGA AAAGGCTCATGGAAGAAAATGCTGCCCTCCAGGCCCAGATACCAGAGCTCCAGAAATCCGGAGCAGTCAA AGAGCATGAACCACTCCGAAAGCCCTCAGAGGCCCAAGAGCCCCCAGAGTCCCCAGAGTTCCCAGCAGCC AGGGAGTCACAGAACCCCTGGGAACCCCCAGCAACCACAGAGCCCGGGGAGCCCACAAAGATCAGAGAGC CCAGGGAACCCTCAGCCATCAGTGAGCTCAGGGAACCCCCAGAGATCAAGGAGCCCCAGGAGCCCCCAGA GACCAATGAGTCTGGAGAATCCTCAGCCATCACAGAGTTCAGGGGATCCCCAGAGATCAAGGAGCCCCAT TTGCCCCCAAAGTCCAAGGAGTACTGGGGACCTCAGGAGCTTCCAATGGTCAAGGAATCCTGGGAGCCCC CAGAGGCCCTGGATTCCACAGCCTGGAAGCCACCAGCAGTCAAGGAATCCCAGGAGATCCAGAAGGCTCT GGAGTCCCCAGCAACCCAGAGGCCCCAGGCGTCCCCAAGGGGTTATGATCTCCCAGCAGTCTGTGAGGCG GAGCCCACAGACAACCAGGGTACCCCAGCCCTCAAGGAGCTTCAGAGTCCACAGCTCCACAACCCTACAA ATGATGAGGAGTCTCAGACGGTTCCAGAATACCAGGAGACCTCATCACAGCTGGAGCCCCTTGAGCATCC ACCTCCCCAGGAGACTCTGGAGCCTCGGGTGCCCCAGGAGCCCCTGGACCCCTCAGATGCCGAGGAGTTT CTAGAACTCTCAGTACCTGAAGAGTCCCTAGAGGGCCTGATAGTTGCGAGAACAGGAACAGAACAGGCCC AGTGTGAATTAGAGGCTACAACTTTGCCCCTAGAGTACCCTTTGGCCTTCAGTGAGGATTTTCAGAAACT CTCTGAGTTTTTAGTTCAGTTGACCAGTTACCTGAGAAGCAGAGGGTATCCCACTGAAGCGGCTCTAGTG AGCTTTGTTGGCAGCTTCTTCTCAGGTGAGGCGGGAAGGATGTTCCAGCCCTTATTAGATAGCCAACCCC CCCTGGTGGAGCAATTTGAAAGGCTCCTGCGAGCGCTCCAAGATACTTTTGACAATCCAGAAAGCTTGGA AGTTGCTAACCAGGGCCTCCCTCAACTTCGCCAAGGGGAAGGCCTTGCTCCCAGGTACTCAACCCGCTTC CATCTCATTGCGCAAGAGTTAGATTTGGGTGAAAGCACGCTCTGCATCCAATTTCAAGAAGAGCTTGCCA GTTCTATTCAAAATGAACTGTCTTGCACAAGTCCAGCCACCAACCTATCTGATGTGATCATTGAGTGTGT CACCCTAGAAGAGAAGGCAAGTGGCGGGGTTGATTCCAGTTCATCATCGTCTGAAGAGGAAAATGGGTCC GAGGGTCCTCCCACTGAAAACCAACCTGTTCAAGCTACAAGTAATCGCCCCCACCTCAGCGAGGCTGAAC GGGCAAGACGCCGCGAAGGCCACTTGTGCCTCTACTGTGGCCATCCAGGTCATTTTGCCAGAGACTGCCC TGTCAAGCCTCATCGTGTCCAGCAGGCGGGAAACATGGAGGCCCGGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208781 representing NM_199468
Red=Cloning site Green=Tags(s) MVEDLAASYVTLKLENEILQAQVKRLMEENAALQAQIPELQKSGAVKEHEPLRKPSEAQEPPESPEFPAA RESQNPWEPPATTEPGEPTKIREPREPSAISELREPPEIKEPQEPPETNESGESSAITEFRGSPEIKEPH LPPKSKEYWGPQELPMVKESWEPPEALDSTAWKPPAVKESQEIQKALESPATQRPQASPRGYDLPAVCEA EPTDNQGTPALKELQSPQLHNPTNDEESQTVPEYQETSSQLEPLEHPPPQETLEPRVPQEPLDPSDAEEF LELSVPEESLEGLIVARTGTEQAQCELEATTLPLEYPLAFSEDFQKLSEFLVQLTSYLRSRGYPTEAALV SFVGSFFSGEAGRMFQPLLDSQPPLVEQFERLLRALQDTFDNPESLEVANQGLPQLRQGEGLAPRYSTRF HLIAQELDLGESTLCIQFQEELASSIQNELSCTSPATNLSDVIIECVTLEEKASGGVDSSSSSSEEENGS EGPPTENQPVQATSNRPHLSEAERARRREGHLCLYCGHPGHFARDCPVKPHRVQQAGNMEARR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199468 |
ORF Size | 1659 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199468.2 |
RefSeq Size | 3019 bp |
RefSeq ORF | 1662 bp |
Locus ID | 213436 |
UniProt ID | Q6P1Y1 |
MW | 61.4 kDa |
Gene Summary | May function as a transcriptional regulator (Probable). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (PubMed:27446912).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A neofunctionalized gene ZCCHC5 is expressed in cartilage and its downregulation inhibits COL2A1 expression and enhances the expression of MMP-13 in chondrocytes
,Khan, N;Haqqi, T;,
Osteoarthritis and Cartilage
[RTL3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218877 | Zcchc5 (untagged) - Mouse zinc finger, CCHC domain containing 5 (Zcchc5), (10ug) |
CNY 7,030.00 |
|
MG208781 | Zcchc5 (tGFP-tagged) - Mouse zinc finger, CCHC domain containing 5 (Zcchc5) |
CNY 6,080.00 |
|
MR208781L3 | Lenti ORF clone of Zcchc5 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 5 (Zcchc5) |
CNY 7,410.00 |
|
MR208781L4 | Lenti ORF clone of Zcchc5 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 5 (Zcchc5) |
CNY 7,410.00 |