Fastkd3 (BC042506) Mouse Tagged ORF Clone
CAT＃: MR208992
- TrueORF®
Fastkd3 (Myc-DDK-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412)
ORF Plasmid: tGFP
"BC042506" in other vectors (4)
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CNY 7168.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310010B21Rik; AW048587 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208992 representing BC042506
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCTGAGCTGCTGCTGCTGCTGATAGTCGCCTTCGCCAATCCCAGCTGCCAGGTGCTGTCATCAC TGCGCATGGCTGCAATCCTGGACGACCAGACCGTGTGTGGCCGTGGTGAGCGTCTGGCCCTGGCCCTGGC CCGAGAGCAGATCAATGGGATCATCGAGGTCCCAGCCAAGGCCAGAGTGGAAGTAGACATCTTTGAGCTG CAGCGGGACAGCCAGTACGAGACCACGGACACCATGTGTCAGATCCTGCCCAAGGGGGTTGTATCTGTCT TGGGACCCTCCTCCAGCCCAGCTTCTGCCTCCACCGTGAGCCATATCTGTGGGGAGAAGGAGATTCCCCA CATCAAGGTGGGTCCTGAGGAGACGCCCCGCCTTCAGTACCTTCGCTTCGCATCTGTCAGCCTGTACCCC AGTAATGAAGATGTCAGCCTGGCAGTCTCCCGAATCCTCAAGTCCTTTAACTACCCCTCAGCTAGCCTCA TCTGCGCCAAGGCTGAGTGCCTGCTGCGGCTAGAAGAACTGGTGCGAGGCTTCCTCATCTCCAAGGAGAC ACTGTCCGTGAGGATGCTTGATGACAGCCGGGACCCCACGCCGCTACTCAAGGAGATCCGAGATGACAAA GTGTCCACCATCATCATTGATGCCAATGCGTCCATCTCCCACCTTGTCCTCCGTAAGGCTTCGGAGCTGG GAATGACCTCAGCGTTTTACAAGTACATCCTCACCACCATGGACTTTCCCATCCTGCATCTGGATGGTAT CGTGGAGGACTCCTCCAACATCCTGGGCTTTTCCATGTTCAACACCTCCCACCCCTTCTACCCAGAGTTT GTGCGCAGCCTCAACATGTCCTGGAGGGAGAACTGTGAAGCCAGCACCTATCCTGGCCCTGCGCTGTCCG CAGCCCTGATGTTTGACGCTGTGCACGTGGTGGTAAGCGCTGTCCGAGAACTGAACCGAAGCCAGGAGAT TGGCGTCAAGCCACTGGCCTGCACTTCGGCCAACATTTGGCCCCATGGGACCAGCCTTATGAACTACCTT CGAATGGTAGAGTATGACGGGCTGACCGGGCGGGTTGAGTTCAACAGCAAAGGGCAGAGGACCAACTACA CACTACGCATCCTGGAGAAGTCCCGCCAGGGCCACCGTGAGATAGGGGTGTGGTACTCTAACCGGACCCT GGCCATGAATGCCACCACCCTGGACATCAACCTGTCACAGACTCTAGCCAACAAGACTCTGGTGGTCACA ACTATCCTGGAGAACCCGTATGTTATGCGCCGGCCCAACTTCCAGGCCTTGTCAGGGAATGAGCGCTTCG AGGGCTTCTGCGTGGACATGCTCAGGGAGCTGGCCGAGCTGCTGCGCTTCCGATACCGCCTGCGGTTGGT AGAGGACGGACTCTACGGGGCACCTGAGCCCAACGGTTCCTGGACAGGCATGGTTGGAGAACTCATCAAC CGGAAGGCAGACCTGGCTGTGGCAGCCTTCACCATCACCGCCGAGAGGGAGAAGGTCATCGACTTCTCCA AGCCCTTCATGACCCTGGGGATCAGCATCCTCTACAGGGTGCACATGGGCCGCAAGCCTGGCTACTTCTC CTTCCTGGACCCCTTCTCCCCTGCCGTGTGGCTCTTCATGCTTCTTGCCTACCTGGCTGTCAGCTGTGTC TTGTTCCTGGCTGCCAGGCTGAGCCCTTATGAGTGGTACAACCCACACCCGTGTCTCCGGGCGCGTCCCC ATATCCTGGAGAACCAGTACACGCTGGGCAACAGCCTCTGGTTCCCCGTGGGTGGCTTCATGCAGCAGGG CTCGGAGATCATGCCGCGGGCACTGTCCACACGCTGTGTCAGCGGAGTCTGGTGGGCCTTCACCTTGATC ATCATCTCCTCCTACACGGCCAACCTGGCTGCCTTCCTCACGGTGCAGCGCATGGAGGTGCCGGTGGAGT CGGCTGACGACCTGGCGGATCAGACCAACATTGAGTACGGCACTATCCACGCTGGCTCCACCATGACCTT CTTCCAGAACTCGCGGTACCAGACGTACCAGCGGATGTGGAACTACATGCAATCGAAGCAGCCCAGCGTG TTTGTCAAGAGCACAGAGGAGGGAATCGCCCGCGTCCTCAACTCCCGCTATGCCTTCCTGCTGGAGTCCA CCATGAACGAGTACCACAGGCGCCTCAATTGCAACCTCACCCAGATCGGGGGCCTCCTCGACACCAAGGG CTACGGCATCGGCATGCCGCTGGGCTCCCCTTTCCGGGATGAGATCACACTGGCCATCCTGCAGCTCCAG GAGAACAACAGGCTGGAGATCCTGAAGCGCAAGTGGTGGGAGGGCGGCCGGTGCCCCAAGGAGGAGGACC ACAGGGCCAAAGGTTTGGGCATGGAGAACATTGGCGGCATTTTTGTCGTGCTGATCTGTGGCCTCATCAT TGCTGTCTTCGTGGCGGTCATGGAGTTCATCTGGTCCACGCGGAGGTCAGCGGAGTCCGAGGAGGTGTCG GTGTGCCAGGAGATGCTGCAGGAGCTACGCCACGCCGTGTCTTGCCGAAAGACCTCGCGTTCCCGCCGGC GCCGGCGCCCTGGTGGCCCGAGCCGGGCCCTGCTGTCGCTGCGCGCAGTCCGCGAGATGCGACTCAGCAA CGGCAAGCTCTACTCGGCCGGCGCGGGCGGGGACGCGGGCGCGCACGGGGGTCCGCAGCGCCTCCTGGAC GACCCCGGACCTCCTGGGGGACCCCGGCCCCAGGCTCCCACGCCCTGCACGCACGTGCGCGTCTGCCAGG AGTGCAGGCGCATCCAGGCGCTGCGAGCTTCGGGGGCCGGGGCGCCCCCACGTGGCCTGGGCACCCCAGC CGAAGCCACCAGCCCGCCTCGGCCGCGGCCAGGCCCCACCGGACCCCGCGAGCTGACCGAGCACGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208992 representing BC042506
Red=Cloning site Green=Tags(s) MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFEL QRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYP SNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDK VSTIIIDANASISHLVLRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEF VRSLNMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYL RMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVT TILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELIN RKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCV LFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLI IISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSV FVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQ ENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFIWSTRRSAESEEVS VCQEMLQELRHAVSCRKTSRSRRRRRPGGPSRALLSLRAVREMRLSNGKLYSAGAGGDAGAHGGPQRLLD DPGPPGGPRPQAPTPCTHVRVCQECRRIQALRASGAGAPPRGLGTPAEATSPPRPRPGPTGPRELTEHE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC042506 |
| ORF Size | 2937 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC042506, AAH42506 |
| RefSeq Size | 2522 bp |
| RefSeq ORF | 1715 bp |
| Locus ID | 69577 |
| MW | 92.4 kDa |
| Gene Summary | Required for normal mitochondrial respiration. Increases steady-state levels and half-lives of a subset of mature mitochondrial mRNAs MT-ND2, MT-ND3, MT-CYTB, MT-CO2, and MT-ATP8/6. Promotes MT-CO1 mRNA translation and increases mitochondrial complex IV assembly and activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217862 | Fastkd3 (untagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412), (10ug) |
CNY 6940.00 |
|
| MG208992 | Fastkd3 (tGFP-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) |
CNY 6180.00 |
|
| MR208992L3 | Lenti ORF clone of Fastkd3 (Myc-DDK-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) |
CNY 7510.00 |
|
| MR208992L4 | Lenti ORF clone of Fastkd3 (mGFP-tagged) - Mouse FAST kinase domains 3 (cDNA clone MGC:28398 IMAGE:4023412) |
CNY 7510.00 |
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