Ddi2 (BC031491) Mouse Tagged Lenti ORF Clone
CAT＃: MR209117L4
- LentiORF®
Lenti ORF clone of Ddi2 (mGFP-tagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157)
"BC031491" in other vectors (4)
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CNY 7700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 1110056G13Rik; 1700027M01Rik; 9130022E05Rik; AI604911; AU040698 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR209117).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC031491 |
| ORF Size | 1746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031491 |
| RefSeq Size | 2307 bp |
| RefSeq ORF | 1748 bp |
| Locus ID | 68817 |
| Gene Summary | Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1. Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2. Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218404 | Ddi2 (untagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157), (10ug) |
CNY 6940.00 |
|
| MG209117 | Ddi2 (tGFP-tagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157) |
CNY 6370.00 |
|
| MR209117 | Ddi2 (Myc-DDK-tagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157) |
CNY 5800.00 |
|
| MR209117L3 | Lenti ORF clone of Ddi2 (Myc-DDK-tagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157) |
CNY 7700.00 |
