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Ppm1d (NM_016910) Mouse Tagged ORF Clone
CAT＃: MR209290
- TrueORF®
Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (Ppm1d)
"NM_016910" in other vectors (3)
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CNY 4,456.00
CNY 5,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AV338790; Wip1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209290 representing NM_016910
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCGGACCAGGGCGGGAGGAAGTACATGGAGGACG TAACTCAGATCGTGGTGGAGCCCGAGCCGGCTGCGGAGGACAAGCCGGCGCCGGTACCGCGGCGGGCGCT CGGGTTGCCGGCGACCCCCACTCTCGCCGGCGTCGGGCCATCGGAAAAAGGCCCGATGGCGGCCCGCGAC CCTGCCCCGGACGCCGCGGCCTCGCTACCCGCTGGCCGCTGCTGTCGCCGCCGCTCTTCGGTGGCCTTCT TTGCAGTGTGCGACGGGCACGGCGGTCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCAT CAAGAAGCAGAAAGGCTTCACCTCGTCCGAGCCCGCCAAGGTGTGCGCTGCCATCCGCAAAGGTTTCCTC GCCTGTCACCTCGCCATGTGGAAGAAACTGGCAGAATGGCCAAAGACTATGACAGGTCTTCCCAGCACGT CCGGGACAACTGCCAGTGTGGTTATAATACGGGGCATGAAGATGTATGTAGCGCATGTAGGTGACTCTGG GGTGGTCCTTGGAATTCAGGATGACCCAAAGGATGATTTTGTGAGAGCTGTGGAGGTGACACAAGATCAC AAGCCAGAACTTCCCAAGGAAAGAGAAAGAATCGAAGGACTTGGCGGCAGTGTGATGAACAAGTCTGGAG TGAACCGAGTAGTTTGGAAAAGGCCCCGGCTCACTCACAGTGGACCTGTCAGAAGGAGCACAGTCATTGA CCAGATTCCTTTTCTGGCTGTAGCAAGAGCGCTTGGTGACTTGTGGAGCTATGATTTCTTCAGTGGTAAG TTTGTGGTGTCACCTGAACCAGACACCAGTGTCCACACTCTTGACCCCCGGAAGCACAAGTATATTATCC TGGGAAGTGATGGACTTTGGAATATGGTTCCACCTCAAGATGCCATCTCCATGTGCCAAGACCAAGAGGA GAAAAAATACTTGATGGGTGAGCAAGGACAGTCCTGTGCCAAAATGCTTGTGAATCGAGCACTTGGCCGC TGGAGGCAGCGTATGCTTCGGGCAGATAACACAAGTGCCATCGTAATCTGCATCTCTCCAGAAGTAGACA ACCAAGGGAACTTCACCAATGAAGATGAGCTCTTTCTGAACCTGACTGATAGCCCTACTTACAACAGCCA GGAGACCTGTGTGATGACTTCTTCTCCAAGTTCTACACCACCAATCAAGTCACCGGAAGAAGATGCATGG CCAAGGCTGAGCTCTAAGGACCATATACCTGCCCTTGTTCGCAGTAATGCCTTCTCAGAGAAGTTTTTAG AGGTCCCAGCTGAGATAGCTAGAGGGAATATCCAGACTGTAGTGATGACCTCAAAAGACTCAGAGACACT TGAAGAAAATTGCCCCAAAGCCCTGACTTTAAGGATTCATGATTCTTTGAATAATACTCTGTCAGTTGGC CTCATTCCAACCAATTCAACAAATACTATCATGGACCAAAAAAACTTAAAGATGTCAACTCCAGGTCAAA TGAAAGCTCAAGAAGTTGAAAGAACCCCTCCAGCCAATTTTAAAAGGACATTAGAAGAATCCAACTCTGG CCCCCTTATGAAGAAGCACCGACGAAATGGCTTAAGTCGAAGTAGCGGGGCCCAGGCTTCCAGTCTCCCT ACAGCATCCCAGCGCAGGCACTCTGTCAAACTGACCCTGAGGCGCAGACTCAGGGGCCAGAGGAAGATGG GAAATCCTCTTCTCCACCAGCACCGGAAAACAGTGTGTGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209290 representing NM_016910
Red=Cloning site Green=Tags(s) MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPAAEDKPAPVPRRALGLPATPTLAGVGPSEKGPMAARD PAPDAAASLPAGRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFL ACHLAMWKKLAEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDH KPELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHSGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGK FVVSPEPDTSVHTLDPRKHKYIILGSDGLWNMVPPQDAISMCQDQEEKKYLMGEQGQSCAKMLVNRALGR WRQRMLRADNTSAIVICISPEVDNQGNFTNEDELFLNLTDSPTYNSQETCVMTSSPSSTPPIKSPEEDAW PRLSSKDHIPALVRSNAFSEKFLEVPAEIARGNIQTVVMTSKDSETLEENCPKALTLRIHDSLNNTLSVG LIPTNSTNTIMDQKNLKMSTPGQMKAQEVERTPPANFKRTLEESNSGPLMKKHRRNGLSRSSGAQASSLP TASQRRHSVKLTLRRRLRGQRKMGNPLLHQHRKTVCVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016910 |
| ORF Size | 1794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016910.2 |
| RefSeq Size | 2911 bp |
| RefSeq ORF | 1797 bp |
| Locus ID | 53892 |
| UniProt ID | Q9QZ67 |
| MW | 66.2 kDa |
| Gene Summary | Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203640 | Ppm1d (untagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (Ppm1d), (10ug) |
CNY 2,000.00 |
|
| MR209290L3 | Lenti ORF clone of Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (Ppm1d) |
CNY 7,890.00 |
|
| MR209290L4 | Lenti ORF clone of Ppm1d (mGFP-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (Ppm1d) |
CNY 7,890.00 |
