Ncl (NM_010880) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR210180 representing NM_010880 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGCTCGCAAAGGCTGGCAAAACCCACGGTGAGGCCAAGAAAATGGCTCCTCCTCCAAAGGAGG TGGAAGAGGATAGTGAAGATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGT CATCCCTCAGAAAAAAGGCAAAAAGGCTACCACAACCCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAA AAGGCTGCAGTTCCCACACCAGCTAAGAAAGCAGCTGTGACCCCAGGCAAAAAGGCAGTAGCCACACCAG CTAAGAAAAACATTACACCAGCCAAAGTCATTCCAACACCGGGTAAGAAGGGAGCTGCACAAGCAAAAGC GTTGGTACCAACTCCTGGTAAAAAGGGAGCTGCCACTCCAGCTAAGGGGGCTAAGAACGGTAAGAATGCC AAGAAGGAAGACAGTGATGAGGATGAAGATGAAGAGGATGAAGATGATAGCGATGAGGATGAAGATGATG AGGAAGAGGATGAGTTTGAGCCACCAATAGTAAAAGGAGTGAAGCCAGCAAAAGCAGCTCCTGCTGCTCC TGCCTCAGAGGATGAGGAAGATGATGAGGATGAAGATGATGAGGAAGATGATGATGAAGAGGAGGAAGAT GACTCTGAGGAAGAAGTTATGGAGATCACAACAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTA TGAAAGCCAAGAGTGTGGCTGAGGAGGAGGATGATGAGGAAGAGGATGAAGATGACGAGGATGAGGATGA TGAGGAAGAGGATGACGAAGATGATGATGAGGAAGAAGAGGAGGAAGAACCTGTTAAAGCAGCACCTGGA AAACGGAAGAAGGAGATGACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAGTAGAAGGCTCAG AACCAACTACACCTTTCAATCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTAATGAATTAAAATT TGCCATCAGTGAACTTTTTGCTAAAAATGATCTTGCTGTTGTGGATGTCAGAACTGGTACAAATAGGAAA TTTGGTTATGTGGACTTTGAGTCTGCTGAAGACCTAGAAAAGGCCTTGGAGCTCACTGGTTTAAAAGTGT TTGGCAATGAAATTAAACTAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGCAAGAACACT TCTAGCCAAAAACCTCTCTTTCAACATCACTGAGGATGAATTAAAGGAAGTGTTTGAAGACGCCATGGAG ATCAGATTAGTCAGCCAGGATGGGAAAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTGAAGCTGATG CAGAGAAAAATTTGGAAGAAAAGCAGGGGGCAGAAATTGATGGACGATCTGTTTCACTCTACTATACTGG AGAGAAAGGTCAAAGGCAAGAGAGAACTGGAAAGACCAGCACTTGGAGTGGTGAATCAAAGACTTTGGTT TTAAGTAACCTTTCCTACAGTGCAACAAAAGAAACTCTTGAGGAAGTATTTGAGAAAGCAACTTTTATCA AAGTGCCCCAGAACCCACATGGCAAACCTAAAGGGTATGCATTTATAGAATTTGCTTCATTTGAAGATGC TAAAGAAGCTTTAAATTCCTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAGTTGCAAGGA TCCAATTCGAGAAGTCAACCATCCAAAACTCTGTTTGTCAAAGGTCTGTCTGAGGATACCACTGAAGAGA CCTTAAAAGAATCATTTGAGGGCTCTGTTCGTGCAAGAATAGTCACTGATCGGGAAACTGGTTCTTCCAA AGGGTTTGGTTTTGTAGACTTTAATAGTGAGGAAGATGCCAAAGCTGCCAAGGAGGCCATGGAAGATGGA GAAATTGACGGAAACAAAGTTACCTTGGACTGGGCCAAACCTAAGGGTGAAGGTGGCTTTGGTGGTCGAG GTGGAGGCAGAGGAGGTTTCGGAGGCAGAGGTGGAGGCAGAGGTGGAAGAGGTGGATTTGGAGGAAGAGG CCGGGGAGGCTTTGGAGGTAGAGGAGGCTTCCGAGGCGGCAGAGGAGGAGGGGGAGACTTCAAGCCACAA GGAAAGAAGACGAAGTTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR210180 representing NM_010880 Red=Cloning site Green=Tags(s) MVKLAKAGKTHGEAKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTK KAAVPTPAKKAAVTPGKKAVATPAKKNITPAKVIPTPGKKGAAQAKALVPTPGKKGAATPAKGAKNGKNA KKEDSDEDEDEEDEDDSDEDEDDEEEDEFEPPIVKGVKPAKAAPAAPASEDEEDDEDEDDEEDDDEEEED DSEEEVMEITTAKGKKTPAKVVPMKAKSVAEEEDDEEEDEDDEDEDDEEEDDEDDDEEEEEEEPVKAAPG KRKKEMTKQKEAPEAKKQKVEGSEPTTPFNLFIGNLNPNKSVNELKFAISELFAKNDLAVVDVRTGTNRK FGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFEDAME IRLVSQDGKSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKTSTWSGESKTLV LSNLSYSATKETLEEVFEKATFIKVPQNPHGKPKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLELQG SNSRSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDG EIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDFKPQ GKKTKFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010880
ORF Size	2121 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010880.3, NP_035010.3
RefSeq Size	8257 bp
RefSeq ORF	2124 bp
Locus ID	17975
UniProt ID	P09405
MW	76.7 kDa
Gene Summary	Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).[UniProtKB/Swiss-Prot Function]

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