Ipo4 (BC003469) Mouse Tagged ORF Clone
CAT＃: MR210501
- TrueORF®
Ipo4 (Myc-DDK-tagged) - Mouse importin 4 (cDNA clone MGC:6831 IMAGE:2649233)
ORF Plasmid: tGFP
"BC003469" in other vectors (4)
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CNY 7600.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RanBP4, Imp4a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210501 representing BC003469
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGACTCCCAAGCACTTTGCTGTACAGGTTGTGGACATGCTGGCACTACATCTTCCCCCTGAGA AGCTCTGTCCCCATGTGATGCCCATGCTGGAAGAAGCCTTGCGGAGCGAGGACCCATACCAGCGCAAAGC AGGATTCCTGGTGTTGGCTGTGCTGTCTGATGGTGCAGGCGACCACATCAGGCAGAGACTGCTGTACCCA CTGCTACAGATTGTGTGCAAGGGCCTGGATGACCCCTCACAGATTGTTCGTAATGCTGCTCTGTTCGCCC TGGGCCAGTTTTCAGAGAACTTACAGCCCCATATCAGCAGCTACTCTGAGGAGGTGATGCCCCTTCTCCT CTCCTACCTGAAGTCAGTGCCGATGGGAAACACACACCACCTGGCCAAGGCCTGCTATGCTCTGGAGAAT TTTGTGGAGAACCTAGGGCCCAAAGTGCAGCCCTACCTTCCGGAGCTTATGGAATGTATGCTGCAGCCAT TGAAGAACCCCAGCAAAGCCCGGACCAAGGAGCTAGCTGTGAGCGCCATAGGGGCCATTGCCACAGCGGC CCAGGATTCCCTGCTGCCCTACTTCCCTACCATCATGGATCTTCTCCGGGAGTTCTTGTTGACAGGCCAC GAAGACTTTCATCTTGTGCAGATCCAGAGCCTGGAGACCCTGGGAGTGCTAGCACGAGCCCTGGGAGAGT CCATGAAGCCCCTGGCTGAGGAATGCTGTCAGCTTGGGCTAGGGTTATGCATCCATATAGATGACCCTGA CGTGAGGCGCTGCACGTACAGCCTGTTTGCAGCCTTATCAGGGTTAATGGGAGAGGGCTTGGGACCCTAC CTGCCTCAGATTACCACGCTCATGCTGCTGTCGCTGCGCTCCACTGAGGGCATTGTGCCACAGTATGACG GGATCAGCTCCTTCCTGCTGTTTGATGACGACAGCGAAGCAGAAGAGGAGGAGGAGCTCATGGATGAAGA TATGGAAGAGGAGGGGGATGACTCTGAGATCTCGGGGTATAGTGTGGAGAATGCCTTCTTTGATGAGAAA GAAGACACCTGCACCGCCCTGGGGGAGATCTCCATGAACACCTGTGTGGCTTTTCTCCCATTCATGGATG CCACCTTCGATGAAGTATACAAACTGCTGGAGTGCCCTCATATGAATGTGCGGAAGTCAGCTTACGAAGC GCTGGGACAGTTCTGCTGTGCACTGCATAAGGCCTCTCAGAGAAGCTCCTCAGATCCCAGCAGCAGTCCC GTTCTGCAGACCTCCCTGGCTCGAGTGATGCCAGCTTACATGCAGGCCGTGAAAGTAGAAAGGGAGCGCC CAGTGGTGATGGCCGTGCTGGAGTCCCTGACAGGGGTGCTGCGCACCTGCGGCAGCCTTGCACTGCAGCC TCCTGGGCGGCTCAGTGAGCTCTGCAACGTGCTCAAGGCTGTGCTGCAGAAAAAGACGGCCTGTCAGGAC GCCGAGGAGGATGATGACGAAGATGATGACCAGGCAGAGTATGACGCCATGTTACTAGAACATGCTGGAG AGGCCATTCCCGTCCTGGCAGCCACAGCTGGAGGACACGCCTTCGCTCCCTTCTTTGCCACGTTCTTGCC ATTGTTGCTGTGTAAGACGAAACAGAGCTGTACAGTGGCAGAGAAGTCCTTTGCTGTGGGAACCCTGGCA GAGTCCATTCAGGGTCTAGGGACCGCCTCAGCCCAGTTTGTGTCTCGGCTGTTCCCTGTGCTGTTAAACA ATGCCCGGGAAGCAGACCCTGAGGTGCGGAGCAATGCCATCTTTGGGTTGGGCGTACTGGCAGAGCATGG CGGCTGCCCTGCTCAGGACCACTTCCCTAAGCTACTGGGCCTCCTTTTGCCCCTGCTGGCAAGGGAGCGA CATGATCGAGTCCGTGATAACATCTGCGGGGCTCTTGCCCGTGTACTGATGGCCAGTCCAGTAGGAAAAA CGGAGCCCCAGGTGCTGGCTACCCTGCTACGTGCCCTGCCCCTGAAGGAAGACATGGAGGAGTGGCTCAC TATAGGTCACCTCTTCAGCTTCCTGCACCAGAACAATCCTGAGCAGGTTGTGGATGTGGCTTCAGAACTC CTGCGCATCTGCAGCCTGATCCTGCCGGACAACCGGATCCCTCCAGACACCAAGGCGGCCCTGCTGCTGC TCCTGACGTTCCTGGCCAAGCAGCACACCGACAGCTTCCACACAGCCCTGGGCTCTCTGCCAAATGATAA AGCTCAGGAACTTCAGGCCATGATGGGCCTCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210501 representing BC003469
Red=Cloning site Green=Tags(s) MGETPKHFAVQVVDMLALHLPPEKLCPHVMPMLEEALRSEDPYQRKAGFLVLAVLSDGAGDHIRQRLLYP LLQIVCKGLDDPSQIVRNAALFALGQFSENLQPHISSYSEEVMPLLLSYLKSVPMGNTHHLAKACYALEN FVENLGPKVQPYLPELMECMLQPLKNPSKARTKELAVSAIGAIATAAQDSLLPYFPTIMDLLREFLLTGH EDFHLVQIQSLETLGVLARALGESMKPLAEECCQLGLGLCIHIDDPDVRRCTYSLFAALSGLMGEGLGPY LPQITTLMLLSLRSTEGIVPQYDGISSFLLFDDDSEAEEEEELMDEDMEEEGDDSEISGYSVENAFFDEK EDTCTALGEISMNTCVAFLPFMDATFDEVYKLLECPHMNVRKSAYEALGQFCCALHKASQRSSSDPSSSP VLQTSLARVMPAYMQAVKVERERPVVMAVLESLTGVLRTCGSLALQPPGRLSELCNVLKAVLQKKTACQD AEEDDDEDDDQAEYDAMLLEHAGEAIPVLAATAGGHAFAPFFATFLPLLLCKTKQSCTVAEKSFAVGTLA ESIQGLGTASAQFVSRLFPVLLNNAREADPEVRSNAIFGLGVLAEHGGCPAQDHFPKLLGLLLPLLARER HDRVRDNICGALARVLMASPVGKTEPQVLATLLRALPLKEDMEEWLTIGHLFSFLHQNNPEQVVDVASEL LRICSLILPDNRIPPDTKAALLLLLTFLAKQHTDSFHTALGSLPNDKAQELQAMMGLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC003469 |
| ORF Size | 2274 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003469.1 |
| RefSeq Size | 2833 bp |
| RefSeq ORF | 2276 bp |
| Locus ID | 75751 |
| MW | 103.8 kDa |
| Gene Summary | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. Acts as chaperone for exposed basic domains.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220845 | Ipo4 (untagged) - Mouse importin 4 (cDNA clone MGC:6831 IMAGE:2649233), (10ug) |
CNY 9120.00 |
|
| MG210501 | Ipo4 (tGFP-tagged) - Mouse importin 4 (cDNA clone MGC:6831 IMAGE:2649233) |
CNY 8360.00 |
|
| MR210501L3 | Lenti ORF clone of Ipo4 (Myc-DDK-tagged) - Mouse importin 4 (cDNA clone MGC:6831 IMAGE:2649233) |
CNY 9500.00 |
|
| MR210501L4 | Lenti ORF clone of Ipo4 (mGFP-tagged) - Mouse importin 4 (cDNA clone MGC:6831 IMAGE:2649233) |
CNY 9500.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
