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Cul1 (NM_012042) Mouse Tagged ORF Clone
CAT＃: MR210588
- TrueORF®
Cul1 (Myc-DDK-tagged) - Mouse cullin 1 (Cul1)
ORF Plasmid: tGFP
"NM_012042" in other vectors (4)
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CNY 5,688.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cul-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210588 representing NM_012042
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCAAACAGGAGTCAGAATCCTCATGGACTGAAGCAGATTGGTCTTGACCAGATCTGGGATGACC TCAGAGCTGGAATTCAGCAGGTGTATACCAGACAAAGCATGGCAAAGTCCAGATACATGGAACTCTACAC TCATGTTTATAACTACTGTACTAGTGTGCATCAGTCCAACCAAGCCCGGGGTGCTGGAGTCCCTCCTTCC AAGTCAAAAAAGGGGCAGACTCCTGGGGGAGCTCAGTTTGTTGGCTTGGAGTTATACAAGCGATTGAAGG AATTTTTGAAGAATTATTTGACAAATCTTCTTAAGGATGGAGAAGATTTAATGGATGAGAGTGTCCTGAA GTTCTACACTCAGCAGTGGGAAGATTACCGATTCTCCAGCAAAGTGCTGAATGGAATCTGTGCCTACCTC AATAGACATTGGGTTCGCCGTGAATGTGATGAAGGACGAAAAGGAATATATGAAATCTATTCACTTGCAT TGGTGACATGGAGAGATTGTTTGTTCAGGCCATTGAATAAACAGGTAACAAATGCTGTTTTAAAGTTGAT TGAAAAGGAAAGAAATGGAGAAACCATCAATACAAGACTGATTAGTGGAGTTGTACAATCTTATGTGGAA TTGGGGCTGAATGAAGATGATGCATTTGCAAAGGGCCCTACGTTAACAGTGTATAAAGAATCCTTTGAAT CTCAATTTTTGGCTGACACAGAGAGATTTTATACCAGAGAAAGTACTGAATTCTTACAGCAGAACCCAGT TACTGAATATATGAAAAAGGCTGAAGCTCGTTTGCTTGAGGAACAGCGGAGAGTTCAGGTTTACCTCCAC GAGAGCACACAAGATGAACTAGCACGGAAATGTGAGCAGGTCCTCATTGAAAAACACTTGGAAATTTTTC ACACAGAGTTTCAGAATTTATTGGATGCTGACAAGAATGAAGATTTGGGCCGCATGTATAATCTTGTATC TAGAATTCAGGATGGCCTAGGAGAATTGAAAAAACTACTAGAGACACATATTCATAATCAGGGTCTTGCA GCAATTGAGAAGTGTGGAGAAGCTGCTCTAAATGACCCCAAGATGTATGTGCAGACAGTGCTGGATGTTC ATAAAAAATACAATGCCCTGGTGATGTCAGCATTCAACAACGATGCTGGCTTCGTAGCTGCTCTTGATAA GGCTTGTGGTCGCTTCATAAACAACAATGCGGTTACCAAAATGGCCCAGTCATCCAGTAAATCCCCTGAG CTGCTTGCTCGGTACTGTGACTCCTTGCTGAAGAAAAGTTCCAAGAACCCAGAAGAGGCAGAACTAGAAG ACACCCTCAATCAAGTGATGGTGGTCTTCAAGTACATCGAGGACAAAGATGTATTTCAGAAGTTCTATGC AAAGATGCTTGCCAAGAGACTGGTACATCAAAACAGTGCGAGTGACGATGCTGAAGCAAGCATGATCTCC AAGTTAAAGCAAGCTTGTGGTTTTGAGTACACTTCCAAACTTCAGCGAATGTTTCAAGACATTGGTGTAA GCAAAGATCTGAATGAACAGTTCAAAAAACACCTGACGAATTCAGAACCGCTGGACTTGGACTTCAGTAT TCAGGTCCTGAGTTCTGGATCGTGGCCCTTTCAGCAGTCTTGCACGTTTGCCTTGCCGTCAGAGTTGGAG CGCAGTTATCAGCGATTTACAGCTTTCTACGCCAGCCGTCACAGTGGCAGAAAATTGACATGGTTGTATC AACTGTCCAAAGGGGAACTAGTCACTAACTGCTTCAAAAACAGATACACTTTGCAGGCGTCCACATTTCA GATGGCAATCCTGCTTCAGTATAACACAGAAGACGCCTATACTGTGCAGCAGTTGACTGACAGCACTCAG ATTAAAATGGACATTTTGGCACAAGTCCTACAGATTTTACTGAAGTCGAAATTGCTGGTCTTAGAAGATG AAAATGCCAATGTTGATGAGGTGGAATTGAAGCCAGACACCTTAATAAAGTTATATCTTGGTTATAAAAA TAAGAAATTGAGGGTTAATATCAATGTACCAATGAAAACTGAACAGAAGCAGGAACAAGAAACTACACAC AAAAATATAGAGGAAGACCGCAAACTACTGATTCAGGCGGCCATCGTGAGAATCATGAAAATGAGGAAGG TCCTGAAACACCAGCAGTTACTTGGTGAAGTCCTCACTCAGCTCTCCTCCAGGTTCAAACCTCGGGTCCC GGTGATCAAGAAATGCATTGATATTCTCATCGAAAAAGAATATTTGGAGCGAGTCGATGGTGAGAAGGAC ACCTACAGTTACTTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210588 representing NM_012042
Red=Cloning site Green=Tags(s) MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPS KSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYL NRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVE LGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLH ESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLA AIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPE LLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMIS KLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELE RSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQ IKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTH KNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKD TYSYLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012042 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012042.4 |
| RefSeq Size | 3171 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 26965 |
| UniProt ID | Q9WTX6 |
| MW | 90.1 kDa |
| Gene Summary | Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221493 | Cul1 (untagged) - Mouse cullin 1 (Cul1), (10ug) |
CNY 9,980.00 |
|
| MG210588 | Cul1 (tGFP-tagged) - Mouse cullin 1 (Cul1) |
CNY 6,370.00 |
|
| MR210588L3 | Lenti ORF clone of Cul1 (Myc-DDK-tagged) - Mouse cullin 1 (Cul1) |
CNY 7,700.00 |
|
| MR210588L4 | Lenti ORF clone of Cul1 (mGFP-tagged) - Mouse cullin 1 (Cul1) |
CNY 7,700.00 |
