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Arnt (NM_001037737) Mouse Tagged ORF Clone
CAT＃: MR210673
- TrueORF®
Arnt (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1
ORF Plasmid: tGFP
"NM_001037737" in other vectors (4)
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CNY 5,800.00
CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b; mKIAA4051; W08714 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210673 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGACTACAGCTAACCCAGAAATGACATCAGATGTACCATCGCTGGGTCCCACCATTGCTTCTG GAAACCCTGGACCTGGGATTCAAGGTGGAGGAGCTGTTGTACAGAGGGCTATTAAGCGACGGTCAGGGCT GGATTTTGATGATGAAGTAGAAGTGAACACTAAATTTTTGAGATGCGATGATGACCAGATGTGTAATGAC AAAGAGCGGTTTGCCAGGTCGGATGATGAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATC ATAGTGAAATAGAACGGCGGCGACGGAACAAGATGACAGCTTACATCACAGAACTGTCAGACATGGTACC TACATGTAGTGCCCTGGCTCGAAAACCAGACAAGCTAACCATCTTACGCATGGCCGTTTCTCACATGAAG TCCTTGAGGGGAACTGGCAACACATCTACTGATGGCTCCTACAAGCCATCTTTCCTCACTGATCAGGAAC TGAAACATTTGATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCCTGTGAGACTGGACGGGTGGT GTATGTCTCTGACTCAGTGACTCCCGTTTTGAACCAGCCACAGTCTGAATGGTTCGGGAGCACACTGTAT GATCAGGTGCACCCAGATGATGTGGATAAACTTCGAGAGCAGCTCTCTACATCAGAAAATGCCCTAACAG GGCGGGTCCTGGATCTGAAGACTGGAACAGTGAAAAAGGAAGGCCAGCAGTCTTCCATGAGGATGTGCAT GGGCTCACGAAGGTCGTTCATCTGCCGCATGAGGTGTGGTACTAGCTCCGTGGACCCTGTTTCCATGAAT AGACTGAGCTTTTTGAGGAACAGATGCAGGAATGGGCTTGGCTCTGTGAAGGAAGGAGAACCTCACTTTG TGGTAGTCCACTGCACAGGCTACATCAAGGCCTGGCCACCAGCAGGTGTCTCCCTCCCAGATGATGACCC AGAGGCTGGCCAGGGGAGCAAATTCTGCCTAGTGGCCATTGGCAGGCTGCAGGTAACTAGTTCTCCCAAC TGTACAGACATGAGTAACATTTGTCAGCCAACAGAGTTCATCTCCCGACACAACATTGAAGGGATATTCA CTTTTGTAGACCATCGTTGTGTGGCTACTGTTGGCTACCAGCCACAGGAGCTCTTAGGGAAGAATATTGT AGAATTTTGTCATCCTGAAGACCAACAACTTCTAAGAGACAGCTTTCAGCAGGTGGTGAAATTAAAAGGT CAGGTGCTGTCCGTCATGTTCCGATTCCGATCTAAGACCCGAGAATGGCTGTGGATGAGAACGAGCTCCT TTACCTTCCAAAACCCTTATTCAGATGAAATTGAGTATATTATCTGCACCAACACCAATGTGAAGAACTC TAGCCAGGAACCACGGCCTACACTGTCCAACACCATCCCAAGGTCACAGCTAGGTCCGACAGCCAATTTA TCCCTAGAGATGGGTACAGGGCAGCTGCCATCCAGGCAGCAGCAGCAGCAGCACACAGAACTGGATATGG TACCAGGAAGAGATGGGCTGGCCAGCTATAATCATTCCCAGGTTTCTGTCCAGCCTGTGGCAAGTGCAGG ATCAGAACACAGCAAGCCCCTTGAGAAGTCAGAAGGTCTCTTTGCACAGGACAGAGATCCAAGGTTTCCA GAAATCTATCCCAGCATCACTGCAGATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAAC AGCTGTTCTCCCAGGGCAGCTCATTCCCTCCTAACCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGTCT TACCCCTCCTGTAACCATTGTCCAGCCATCATCTTCTGCAGGGCAGATACTGGCCCAGATTTCACGTCAC TCCAACCCTGCCCAGGGATCAGCGCCGACCTGGACCTCTAGCTCCCGCCCAGGCTTTGCCGCCCAGCAGG TGCCCACCCAGGCTACAGCCAAGACTCGTTCTTCCCAATTTGGTGTGAACAACTTTCAGACTTCTTCCTC CTTCAGTGCTATGTCTCTTCCGGGTGCTCCCACTGCCTCATCTGGTACTGCTGCCTACCCTGCTCTCCCC AACCGTGGCTCCAACTTTCCTCCTGAGACTGGACAGACCACAGGACAGTTCCAGGCCCGGACAGCAGAGG GCGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCCCATCATCGGTCTAGTTCCAGTGAGCAGCATGT TCAGCAGACACAAGCACAAGCACCTAGCCAGCCTGAGGTCTTTCAAGAAATGCTGTCCATGCTGGGAGAC CAAAGCAACACCTACAACAATGAAGAATTTCCTGATCTAACTATGTTTCCCCCCTTTTCCGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210673 protein sequence
Red=Cloning site Green=Tags(s) MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRRSGLDFDDEVEVNTKFLRCDDDQMCND KERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMK SLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLY DQVHPDDVDKLREQLSTSENALTGRVLDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDPVSMN RLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPN CTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKG QVLSVMFRFRSKTREWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIPRSQLGPTANL SLEMGTGQLPSRQQQQQHTELDMVPGRDGLASYNHSQVSVQPVASAGSEHSKPLEKSEGLFAQDRDPRFP EIYPSITADQSKGISSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQILAQISRH SNPAQGSAPTWTSSSRPGFAAQQVPTQATAKTRSSQFGVNNFQTSSSFSAMSLPGAPTASSGTAAYPALP NRGSNFPPETGQTTGQFQARTAEGVGVWPQWQGQQPHHRSSSSEQHVQQTQAQAPSQPEVFQEMLSMLGD QSNTYNNEEFPDLTMFPPFSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001037737 |
| ORF Size | 2376 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001037737.2 |
| RefSeq Size | 4370 bp |
| RefSeq ORF | 2376 bp |
| Locus ID | 11863 |
| UniProt ID | P53762 |
| MW | 87 kDa |
| Gene Summary | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205717 | Arnt (untagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG210673 | Arnt (tGFP-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1 |
CNY 7,700.00 |
|
| MR210673L3 | Lenti ORF clone of Arnt (Myc-DDK-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1 |
CNY 8,930.00 |
|
| MR210673L4 | Lenti ORF clone of Arnt (mGFP-tagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1 |
CNY 8,930.00 |
