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Ephb4 (NM_010144) Mouse Tagged ORF Clone
CAT＃: MR211390
- TrueORF®
Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 2
ORF Plasmid: tGFP
"NM_010144" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI042935; Htk; MDK2; Myk1; Tyro11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211390 representing NM_010144
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCCGAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAGAAGAGACCCTGTTGAACACAA AACTGGAAACGGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGGCCAGTGGGAGGAGCTAAGCGG CCTGGATGAGGAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATGAAGCGTCCAGGGGGCCAGGCT CACTGGCTGCGCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGTATGCCACGATACGCTTCACCA TGATGGAATGCCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGACATTCACTGTCTTCTATTACGA GAGCGAAGCTGATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAACCCCTACATCAAGGTGGACACA GTGGCCGCAGAACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAGGGAAAGTTAATATCAAGACGC TGCGCCTGGGTCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGACCAAGGAGCCTGCATGGCTCT GCTCTCCCTGCATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAACTTGACCTACTTCCCCGAGACG GTGCCTCGGGAGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACGCGGTCCCTACCGCCAACCCCA GCCCCAGCCTCTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGTCACGGGCTGCAGCTGCGCGCC AGGGTACGAGGCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAGGGAACCTTCAAGCCCCAAATA GGAGACGAGTCCTGCCTGCCATGCCCAGCCAACAGCCACTCGAATAACATTGGGTCTCCTGTCTGCCTGT GTCGAATTGGGTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTGCACTACCCCACCCTCTGCTCC AAGAAGCGTGGTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGGAGTGCTCCCCTTGAGTCCGGA GGCCGAGAGGACCTCACTTATGCTGTACGCTGCCGAGAGTGCCGTCCTGGGGGTTCCTGCTTGCCCTGTG GGGGCGACATGACCTTCGACCCCGGTCCTCGAGACCTGGTTGAGCCCTGGGTGGCAATCCGAGGGCTGCG TCCTGATGTCACCTATACCTTTGAGGTTGCTGCTTTGAATGGTGTGTCTACCTTAGCCACTGGACCACCT CCTTTTGAGCCTGTCAATGTCACCACTGACCGTGAGGTGCCTCCTGCAGTGTCTGACATCCGAGTGACTC GGTCGTCACCCAGCAGCTTGATCCTGTCATGGGCTATCCCCAGAGCACCCAGTGGGGCCGTGCTGGACTA CGAGGTCAAGTATCATGAGAAGGGCGCAGAGGGCCCCAGCAGTGTTCGTTTCCTGAAGACATCAGAAAAC CGAGCTGAGCTCCGGGGGCTGAAGCGGGGAGCCAGCTATCTGGTCCAGGTACGCGCACGGTCCGAGGCTG GCTACGGTCCCTTCGGCCAGGAGCATCACAGTCAGACTCAACTGGATGAGAGCGAGAGCTGGCGGGAGCA GCTGGCCCTGATTGCAGGCACTGCGGTTGTGGGTGTGGTCCTGGTCCTGGTGGTCGTCATCATTGCAGTT CTCTGCCTCAGGAAGCAGAGCAATGGGAGGGAAGTTGAGTACTCGGATAAGCATGGGCAGTATCTCATCG GGCACGGTACCAAGGTCTACATTGATCCTTTTACTTACGAAGACCCTAATGAGGCAGTGAGGGAATTTGC CAAAGAGATCGATGTCTCCTATGTCAAGATTGAAGAGGTAATTGGTGCAGGTGAGTTCGGCGAGGTGTGC CGGGGTCGGCTGAAGGCACCAGGGAAAAAGGAGAGCTGTGTGGCCATCAAGACTCTGAAGGGTGGCTACA CCGAGCGCCAGAGGCGTGAGTTCCTGAGCGAGGCCTCCATCATGGGCCAGTTCGAGCATCCCAACATCAT CCGCCTCGAGGGCGTGGTCACCAACAGTGTGCCGGTTATGATCCTCACGGAATTCATGGAGAACGGAGCC CTGGACTCCTTCCTGCGGCTGAACGACGGGCAGTTCACAGTCATCCAGCTGGTGGGCATGCTGAGGGGCA TCGCCTCGGGCATGCGGTACCTGGCTGAAATGAGCTATGTCCACCGAGACCTGGCTGCTCGGAACATCTT GGTCAACAGTAACCTGGTCTGCAAGGTGTCCGACTTTGGCCTCTCCAGATTCTTGGAGGAGAACTCCTCT GATCCCACCTACACAAGTTCCCTGGGAGGCAAGATTCCCATCCGATGGACCGCCCCTGAAGCCATTGCCT TCAGGAAGTTCACCTCTGCCAGTGATGCCTGGAGCTATGGGATCGTCATGTGGGAGGTCATGTCTTTTGG GGAACGGCCATACTGGGACATGAGCAACCAGGATGTGATCAATGCCATTGAACAGGACTACCGGCTGCCT CCTCCTCCAGACTGCCCCACCTCCCTCCACCAGCTCATGCTGGATTGTTGGCAGAAGGACCGGAATGCCC GGCCCCGCTTTCCCCAGGTGGTCAGCGCTCTGGACAAGATGATCCGGAATCCCGCTAGCCTCAAAATCGT GGCCAGGGAGAATGGCGGGGCCTCACATCCACTCTTGGACCAACGGCAGCCTCACTATTCTGCTTTCGGT TCTGTGGGCGAGTGGCTTCGAGCCATCAAGATGGGAAGATACGAGGAAAGTTTTGCAGCGGCTGGATTCG GCTCCTTTGAGGTGGTCAGTCAGATCTCTGCCGAGGACCTTCTCCGAATTGGAGTCACTCTGGCAGGACA CCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAAGCTAAGCCAGGAGCCCCTGGTGGGACA GGGGGACCAGCCCAGCAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211390 representing NM_010144
Red=Cloning site Green=Tags(s) MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQA HWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDT VAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPET VPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQI GDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESG GREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPP PFEPVNVTTDREVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSEN RAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVLVLVVVIIAV LCLRKQSNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVC RGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMILTEFMENGA LDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSS DPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLP PPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFG SVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGAPGGT GGPAQQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010144 |
| ORF Size | 2961 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010144.6, NP_034274.4 |
| RefSeq Size | 4334 bp |
| RefSeq ORF | 2964 bp |
| Locus ID | 13846 |
| UniProt ID | P54761 |
| MW | 109.3 kDa |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222990 | Ephb4 (untagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 2, (10ug) |
CNY 7,240.00 |
|
| MG211390 | Ephb4 (tGFP-tagged) - Mouse Eph receptor B4 (Ephb4) |
CNY 7,890.00 |
|
| MR211390L3 | Lenti ORF clone of Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 2 |
CNY 9,030.00 |
|
| MR211390L4 | Lenti ORF clone of Ephb4 (mGFP-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 2 |
CNY 9,030.00 |
