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Phc1 (NM_007905) Mouse Tagged ORF Clone
CAT＃: MR211438
- TrueORF®
Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1
ORF Plasmid: tGFP
"NM_007905" in other vectors (4)
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CNY 10,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW557034; Edr; Edr1; Mph1; Rae-28; rae28 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211438 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACGGAGAGTGAGCAGAACTCTAGTTCGACCAATGGGAGTTCCAGTTCAGGGGCCAGCTCTCGGC CCCAGATAGCACAGATGTCCCTGTATGAACGGCAAGCAGTGCAGGCTCTGCAAGCACTGCAGCGGCAACC TAATGCGGCTCAATATTTCCACCAGTTCATGCTCCAGCAACAGCTCAGCAATGCCCAACTGCACAGCCTG GCAGCTGTCCAGCAGGCCACGATTGCTGCCAGTCGACAGGCTAGCTCCCCAAACAGCAGCACTGCACAGC AGCAGACCGCTACCACCCAGGCCTCGATGAATCTCGCGACCACGTCAGCTGCTCAGCTCATCAGCCGATC GCAGAGTGTGAGCTCCCCCAGTGCTACCACCCTGACGCAGTCGGTGCTGCTGGGGAACACCACTTCCCCA CCTCTCAACCAGTCCCAGGCCCAGATGTATCTGCGGCCACAGCTGGGCAACCTATTGCAGGTTAACCGGA CCTTGGGCCGGAATGTGCCTCTTGCCTCCCAGCTCATCCTGATGCCTAATGGGGCAGTGGCTGCAGTCCA GCAGGAGGTGCCACCCGCTCAGTCTCCTGGAGTTCATGCAGATGCAGATCAGGTGCAGAACTTGGCAGTG AGGAACCAACAGGCTTCAGCTCAAGGACCCCAAATGCCAGGCTCCACTCAGAAGGCCATCCCTCCTGGAG CTTCTCCTGTTTCTGGCCTCTCCCAGACTTCTAGCCAGGCCCTAGCCGTGGCACAGGCTTCTTCTGGGGC CTCAGGCCAGTCACTCAACCTTAGTCAAGCCGGTGGAGGCAGTGGGAATAGCCTCCCAGGCTCCATGGGT CCAGGTGGAGGTGGCCAGGCGCCTGGGGGTTTAGGTCAGTTGCCTTCTTCAGGATTGACTGGTGGAAGCT GTCCCCGGAAGGGCACAGGAGTGGTGCAGCCCTTACCTGCAGCTCAAACAGTGACTGTGAGCCAAGGAAG CCAAACAGAAGCAGAAAGTGCAGCAGCCAAGAAGGCAGAAGCAGATGGGAGTGGCCAGCAGAGTGTGGGC ATGAACCTGACTCGGACAGCCACACCTGCCCCCAGCCAGACGCTTATTAGCTCAGCCACATACACACAGA TCCAGCCCCATTCACTGATTCAGCAACAGCAGCAGATCCACCTCCAGCAGAAGCAAGTGGTGATCCAGCA GCAGATTGCCATCCACCACCAGCAACAGTTCCAACACCGCCAGTCCCAGCTGCTTCACACAGCCACACAC CTCCAGTTGGCCCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGC AGCAGCAAGGAACAACCCTCACTGCCCCTCAGCCACCCCAGGTCCCACCTACTCAGCAGGTCCCACCTTC CCAATCACAACAGCAAGCCCAGACTCTGGTGGTCCAACCCATGCTTCAGTCTTCACCCCTGACTCTTCCA CCTGAACCAACCTCTAAACCACCCATCCCCATTCAGTCCAAACCTCCTGTAGCGCCTATCAAACCTCCTC AACTAGGAGCTGCTAAGATGTCAGCTACACAGCAGCCACCACCACATATCCCCGTGCAAGTTGTCGGTAC CCGGCAGCCAGGTTCAGCCCAAGCACAGGCTTTGGGATTAGCACAGCTGGCTGCTGCTGTACCTACTCCC CGTGGAATAACAGGAGCAGTCCAGCCTGGCCAGGCTCATCTGGCCTCCTCGCCACCTTCATCACAGGCGG CTCCTGGTGCACTTCAAGAATGTCCTCCTGCATTGGCTGCTGGGATGACCCTTGCTCCTGTGCAGGGGAC AGCACATGTGGTAAAGGGTGGGCCTACAGCCTCCTCTCCTGTTGTGGCCCAGGTCCCTGCTGCTTTCTAC ATGCAGTCGGTGCACCTGCCGGGTAAAGCCCAGACCTTGGCTGTGAAACGCAAAGCTGAGTCTGAGGAGG AGAGAGATGACCTCTCCGCATTGGCCTCTGTGCTTCCTACAAAGGCCTCCCCAGCAGCAGAGAGCCCAAA GGTAATAGAGGAGAAGAATAGTCTTGGAGAGAAAGCTGAACCAGTGGCCAGTTTGAATGCTAATCCTCCA AACAGTGATCTAGTAGCCTTGGCCCCTACCCCATCAGCACCACCTCCTACTCTGGCCTTGGTGTCCAGAC AGATGGGTGACTCAAAACCCCCACAGGCCATTGTGAAGCCCCAGATTCTCACGCACATCATTGAAGGCTT TGTTATCCAGGAAGGAGCAGAGCCTTTTCCGGTGGGATGTTCTCAGTTCCTGAAAGAAACCAAGAAGCCA CTGCAGGCTGGCCTTCCGACCGGGCTGAATGAGAGTCAGCCAAGTGGCCCTTTGGGGGGAGACAGCCCCT CTGTAGAGTTAGAGAAGAAGGCAAATCTCCTCAAGTGTGAGTACTGTGGCAAGTACGCCCCAGCAGAGCA GTTTCGAGGCTCTAAGAGGTTCTGCTCCATGACGTGTGCAAAGAGGTACAATGTGAGCTGCAGCCACCAG TTCCGCTTGAAGAGGAAAAAAATGAAAGAGTTTCAGGAAGCCAGCTATGCACGTGTTCGGAGGCGTGGAC CCCGCCGCAGCTCCTCTGATATTGCTCGTGCTAAGATCCAGGGCAAGCGCCATCGGGGTCAAGAGGACTC TAGCCGGGGATCAGATAATTCCAGCTATGATGAAGCACTCTCTCCAACATCTCCTGGGCCACTGTCAGTA AGAGCCGGGCACGGAGAACGTGATCTAGGAAACACCATTACAACCCCATCCACGCCAGAGTTACAGGGCA TCAATCCCGTGTTCTTGTCCAGTAATCCTAGCCAATGGAGCGTCGAGGAGGTCTACGAGTTTATTGCTTC TCTGCAAGGCTGCCAAGAGATTGCAGAAGAGTTTCGTTCCCAGGAGATTGATGGACAGGCCCTTTTATTA CTTAAAGAAGAACATCTGATGAGTGCCATGAACATCAAATTAGGTCCTGCTCTCAAGATCTGTGCCAAGA TCAATGTCCTCAAGGAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211438 protein sequence
Red=Cloning site Green=Tags(s) METESEQNSSSTNGSSSSGASSRPQIAQMSLYERQAVQALQALQRQPNAAQYFHQFMLQQQLSNAQLHSL AAVQQATIAASRQASSPNSSTAQQQTATTQASMNLATTSAAQLISRSQSVSSPSATTLTQSVLLGNTTSP PLNQSQAQMYLRPQLGNLLQVNRTLGRNVPLASQLILMPNGAVAAVQQEVPPAQSPGVHADADQVQNLAV RNQQASAQGPQMPGSTQKAIPPGASPVSGLSQTSSQALAVAQASSGASGQSLNLSQAGGGSGNSLPGSMG PGGGGQAPGGLGQLPSSGLTGGSCPRKGTGVVQPLPAAQTVTVSQGSQTEAESAAAKKAEADGSGQQSVG MNLTRTATPAPSQTLISSATYTQIQPHSLIQQQQQIHLQQKQVVIQQQIAIHHQQQFQHRQSQLLHTATH LQLAQQQQQQQQQQQQQQQQQQQQQQGTTLTAPQPPQVPPTQQVPPSQSQQQAQTLVVQPMLQSSPLTLP PEPTSKPPIPIQSKPPVAPIKPPQLGAAKMSATQQPPPHIPVQVVGTRQPGSAQAQALGLAQLAAAVPTP RGITGAVQPGQAHLASSPPSSQAAPGALQECPPALAAGMTLAPVQGTAHVVKGGPTASSPVVAQVPAAFY MQSVHLPGKAQTLAVKRKAESEEERDDLSALASVLPTKASPAAESPKVIEEKNSLGEKAEPVASLNANPP NSDLVALAPTPSAPPPTLALVSRQMGDSKPPQAIVKPQILTHIIEGFVIQEGAEPFPVGCSQFLKETKKP LQAGLPTGLNESQPSGPLGGDSPSVELEKKANLLKCEYCGKYAPAEQFRGSKRFCSMTCAKRYNVSCSHQ FRLKRKKMKEFQEASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSDNSSYDEALSPTSPGPLSV RAGHGERDLGNTITTPSTPELQGINPVFLSSNPSQWSVEEVYEFIASLQGCQEIAEEFRSQEIDGQALLL LKEEHLMSAMNIKLGPALKICAKINVLKET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007905 |
| ORF Size | 3033 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007905.3 |
| RefSeq Size | 3899 bp |
| RefSeq ORF | 3033 bp |
| Locus ID | 13619 |
| MW | 106.1 kDa |
| Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202540 | Phc1 (untagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1, (10ug) |
CNY 7,040.00 |
|
| MG211438 | Phc1 (tGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 |
CNY 11,120.00 |
|
| MR211438L3 | Lenti ORF clone of Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 |
CNY 11,970.00 |
|
| MR211438L4 | Lenti ORF clone of Phc1 (mGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 1 |
CNY 11,970.00 |
