Zcchc6 (BC023880) Mouse Tagged ORF Clone
CAT＃: MR211479
- TrueORF®
Zcchc6 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 6 (cDNA clone MGC:38585 IMAGE:5354757)
ORF Plasmid: tGFP
"BC023880" in other vectors (4)
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CNY 10260.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC38585, 6030448M23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211479 representing BC023880
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTTTTCTTCCTTCAGCAAAGAAAAGAACCTCTCTTGCCAGTTTATCTAGGATCATGGATTGAAG AATTCTCATTAAATAAATTAGGGAATTTCAGCCTTAAAGATGTTGAGAAAGACTCTGTGGTCTGGGAATA CACTGACAACAGCACAGGGGACACCAGCTCAGCAAAAGAGGAGGCACCCAAAGAGACTGCAGCAAAGAAG GGACAGGTGCCATTGACATTTAATATAAAGCATCAGCCTTCAGTGCCAGTCGGGCAACTCTGGGTGGAAT TGCTGCGGTTCTATGCTTTAGAGTTTAATCTGGCTGATTTAGTGATCAGTATTCGTGTCAAAGAATTAAT ATCACGGGAATCAAAGGACTGGCCCAAAAAGCGTATCGCAATTGAAGACCCCTATTCTGTTAAAAGAAAT GTGGCAAGGACCCTGAATAACCAGCCTGTGTTTGAGTATATACTCCATTGTTTGAGGACAACATATAAAT ATTTCGCTCTTCCACACAAAGTTACAAAACCCAACCTCACAAAGCCTCCTAGCCCAGTCACCTGTGTTTC AGACCCTTACAGAGAAGCAAAAAATGGTGGCCCAGAACCCCAAGCAACAAATATTGATAAACTGGGAAAT GCAGCTGTGGCTCAAGATCCTGGTGTTCAAACCTCAGGTGACTGCAGAGCACAGCTAGTTACTCTTAAGA ATACCACTGAGGAGGTGGGAAGCCCAGCAAAGGAGAAGACAGGAGGTGTGCACATCCCAGCCCACCAGGA GAGCTCTGGCTGTGTCCAGGCAGAGGTCAGTTGTGAGGGTTTGGAGGATGCCACAGCAGAGCTTCCGGAA ACAGGAAGTGACAACGAGGAAGTCAGAAGGAAGACCAAGCATCCTTTGAGCACTGATGACCAAGGTTTAA GCAGCAGCAAGCACCCAGAACTGCAGAACTGTGGCAGCCTGTGTGGTCTGCAGGCAGACAACACTCTGGA GTTAGTAGCTGAAGAGTGTAATAGCTGTGCCTCTTTGGACAACAAGGCTGAGGTCAATGAAGAGAGGATA GAAGGCGCTGAGGAACTAGAAGAAGCAGCAGCTCTGAGTTGCTTTTCCCCTTCAGTACAGAGTCGCACAT CTGCAGCCATGCACTTTGATGATGAGGAGGAGGAGGAGGAAGAAGAGGAGGAAGAGGAGCCCAGGCTCAG CATTAACCTTACAGAAGATGAGGAGGGGGTTGCTAATGAGCATCAGGTGGACAGCAGGTATGCTGGGTCA GGTGAGGAGGATGCCCTCTCTGAAGAAGATGACTTGGCAGAGCCTGCTAAGGGTGAAGATACAGGAGAAT GTGGAGAAAACGTGGGTGGGACTCTGCTCATAGACCTAAATAGAATAACACTTAAAGAAGAAAGCTTTCC TGAGGAAGATTTACCTGGGGACCAGTCTGAGTTCTTCTACGAATTTAGAAAACTGACCTTCACCAAAGGC AAGTCCCCCACAGTAGTGTGCAGCTTGTGTAAGCGAGAAGGTCACCTAAAGAAGGACTGTCCTGAGGACT TCAAGAGGGTCCAGCTGGAGCCGTTGCCACCCCTGACACCCAAGTTCTCAAACATCTTAGATCAAGTCTG CGTCCAGTGTTACAAGGATTTTTCTCCAACTATTGTAGAAGATCAGGCTCGTGAACATATCCGGCAAAAT CTAGAAAGTTTCATAAAACAGGATTTTCCAGGAACTAAATTGAGCTTGTTTGGCTCCTCCAAAAATGGAT TTGGGTTCAAACAGAGTGACCTTGACGTCTGTATGACGATTAACGGACATGAGACTGCTGAGGGGCTGGA CTGTGTAAGAACTATTGAGGAGCTGGCTCGAGTCCTCAGAAAGCACTCAGGGTTGAGGAATATCCTTCCT ATCACAACAGCCAAGGTGCCCATCGTGAAGTTCTTCCATCTAAGAAGTGGTCTGGAAGTGGACATCAGCT TGTATAACACACTGGCGCTTCATAACACAAGGCTCTTGTCTGCATACTCAGCCATTGACCCCAGAGTGAA GTATCTGTGCTACACCATGAAAGTGTTCACAAAGATGTGTGACATCGGTGACGCGTCCAGAGGCAGCTTG TCATCCTACGCTTACACTCTTATGGTGCTATACTTCCTCCAGCAAAGGTCTCCACCTGTCATCCCCGTGC TTCAAGAGATATACAAAGGTGAAAAGAAACCAGAAATACTTGTTGATGGCTGGAATATTTACTTTTTCGA TCAAATAAATGAACTGCCCACTTGTTGGCCAGAATATGGGAAAAATACAGAACCTGTCGGGCAGCTGTGG CTGGGACTTCTGCGCTTCTACACTGAGGAGTTTGACTTTAAGGAGCACGTGATCAGCATCAGAAGAAAGA GTCTGCTTACAACTTTTAAGAAACAGTGGACCTCAAAATACATTGTTATTGAAGATCCCTTCGATTTGAA TCACAATCTTGGTGCTGGATTATCGAGGAAAATGACAAATTTTATAATGAAAGCTTTTATCAATGGTAGA AGAGTATTTGGAATTCCAGTCAAAGGATTTCCAAAGGACAACCCCTCAAAACTGGCATACTTTTTTGATC CGGATGTCCTAACTGAGGGAGAACTGGCCCCAAATGACAGGTGCTGCCGAATTTGTGGGAAAATCGGGCA CTTCATGAAGGACTGTCCCATGAGGAGGAAAGTGAGGCGACGGAGAGACCAGGAAGATACCCCAAACCAA AGGTACTCTGAGAGCAAAGAAAAAAGAAGCAAAGAGGACAAAGAGATTCAGAACAAGTACACAGAAAAGG AAGTGTCAACAAAAGAAGATAAGCTCACACCGTGTGCAGCTGCGAAAGCCAAGCCAGTGAGGGCAGCTGT CGACCTGGGCCGGGAGAAGCTCCTCAGGACGCCCACAGAAAAGTGGAAGAGACAGGACGACAGAGACTCG AGAGAAAAGCGTTGTTTCATCTGTGGAAGAGAAGGACACATTAAAAAGGAATGCCCACAGTTCAAAGGCT CTCCAGGTAGCCTTTCCAGTAAATATATGACTCAGGGGAGAGCCTCAGTGAAGAGGACCCAGCAGGAATC A AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211479 representing BC023880
Red=Cloning site Green=Tags(s) MAVFFLQQRKEPLLPVYLGSWIEEFSLNKLGNFSLKDVEKDSVVWEYTDNSTGDTSSAKEEAPKETAAKK GQVPLTFNIKHQPSVPVGQLWVELLRFYALEFNLADLVISIRVKELISRESKDWPKKRIAIEDPYSVKRN VARTLNNQPVFEYILHCLRTTYKYFALPHKVTKPNLTKPPSPVTCVSDPYREAKNGGPEPQATNIDKLGN AAVAQDPGVQTSGDCRAQLVTLKNTTEEVGSPAKEKTGGVHIPAHQESSGCVQAEVSCEGLEDATAELPE TGSDNEEVRRKTKHPLSTDDQGLSSSKHPELQNCGSLCGLQADNTLELVAEECNSCASLDNKAEVNEERI EGAEELEEAAALSCFSPSVQSRTSAAMHFDDEEEEEEEEEEEEPRLSINLTEDEEGVANEHQVDSRYAGS GEEDALSEEDDLAEPAKGEDTGECGENVGGTLLIDLNRITLKEESFPEEDLPGDQSEFFYEFRKLTFTKG KSPTVVCSLCKREGHLKKDCPEDFKRVQLEPLPPLTPKFSNILDQVCVQCYKDFSPTIVEDQAREHIRQN LESFIKQDFPGTKLSLFGSSKNGFGFKQSDLDVCMTINGHETAEGLDCVRTIEELARVLRKHSGLRNILP ITTAKVPIVKFFHLRSGLEVDISLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSL SSYAYTLMVLYFLQQRSPPVIPVLQEIYKGEKKPEILVDGWNIYFFDQINELPTCWPEYGKNTEPVGQLW LGLLRFYTEEFDFKEHVISIRRKSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGR RVFGIPVKGFPKDNPSKLAYFFDPDVLTEGELAPNDRCCRICGKIGHFMKDCPMRRKVRRRRDQEDTPNQ RYSESKEKRSKEDKEIQNKYTEKEVSTKEDKLTPCAAAKAKPVRAAVDLGREKLLRTPTEKWKRQDDRDS REKRCFICGREGHIKKECPQFKGSPGSLSSKYMTQGRASVKRTQQES SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
| ACCN | BC023880 |
| ORF Size | 3081 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023880.1 |
| RefSeq Size | 4215 bp |
| RefSeq ORF | 3083 bp |
| Locus ID | 214290 |
| MW | 154.6 kDa |
| Gene Summary | Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:28792939). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of miRNA precursors, including that of let-7 (pre-let-7) (PubMed:22898984). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult (By similarity) (PubMed:22898984). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (By similarity). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 oligouridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (By similarity). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity). Does not play a role in replication-dependent histone mRNA degradation (By similarity). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28792939, PubMed:22898984). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223121 | Zcchc6 (untagged) - Mouse zinc finger, CCHC domain containing 6 (cDNA clone MGC:38585 IMAGE:5354757), (10ug) |
CNY 12920.00 |
|
| MG211479 | Zcchc6 (tGFP-tagged) - Mouse zinc finger, CCHC domain containing 6 (cDNA clone MGC:38585 IMAGE:5354757) |
CNY 11310.00 |
|
| MR211479L3 | Lenti ORF clone of Zcchc6 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 6 (cDNA clone MGC:38585 IMAGE:5354757) |
CNY 12160.00 |
|
| MR211479L4 | Lenti ORF clone of Zcchc6 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 6 (cDNA clone MGC:38585 IMAGE:5354757) |
CNY 12160.00 |
