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Zfp521 (NM_145492) Mouse Tagged ORF Clone
CAT＃: MR211913
- TrueORF®
Zfp521 (Myc-DDK-tagged) - Mouse zinc finger protein 521 (Zfp521)
"NM_145492" in other vectors (3)
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CNY 9,128.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B930086A16Rik; Evi3; Znf521 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211913 representing NM_145492
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGATTG AAGATGGGGAGGCAGTAGACTGCAAGAAGAGGCCAGAAGACGGGGAGGAGTTGGAGGAGGACGCTGTGCA CAGCTGTGACAGCTGCCTCCAGGTGTTTGAGTCACTGAGCGACATCACAGAGCACAAGATCCATCAGTGC CAGCTGACAGATGGCGTTGACGTTGAGGACGACCCCAGCTGCTCTTGGCCAGCCTCCTCGCCTTCCAGTA AGGACCAGACTTCCCCCAGCCATGGAGAAGGGTGTGATTTCGGAGAGGAAGAAGGTGGCCCTGGACTGCC TTACCCATGCCAGTTCTGTGACAAGTCCTTTAGCCGCCTCAGCTACCTAAAGCACCATGAACAGAGCCAC AGTGACAAGCTGCCCTTCAAGTGCACCTACTGCAGTCGGCTGTTCAAACACAAGCGGAGCCGTGACCGCC ACATAAAGCTCCACACTGGGGATAAGAAGTATCACTGTAGCGAGTGTGACGCAGCCTTCTCCCGGAGCGA TCATCTCAAGATCCACTTAAAGACTCACACCTCCAACAAGCCATATAAATGTGCCGTCTGCCGCAGAGGG TTCCTCTCCTCTAGCTCCTTGCACGGACATATGCAGGTTCATGAGCGGAACAAGGACGGCTCCCAGTCTG GCTCCAGGATGGAGGACTGGAAGATGAAGGACACTCAGAAGTGCAGCCAGTGCGAGGAAGGCTTTGACTT CCCGGAAGACCTCCAGAAGCACATTGCAGAATGCCACCCCGAGTGCTCCCCTAATGAGGACCGAGCAGCC CTCCAGTGCATGTACTGCCACGAGCTGTTTGTGGAGGAGACGTCCCTCATGAACCACATCGAGCAGGTAC ACGGGGGTGAGAAGAAGAACTCTTGCAGCATTTGCTCAGAGAGCTTCCTCACGGTCGAGGAGCTGTATAG CCACATGGACAGTCACCAGCAGCCAGAGTCCTGCAATCACAGCAACAGCCCTTCCCTGGTCACTGTGGGT TACACCTCAGTGTCCAGCACGACTCCAGACTCGAACCTCTCAGTGGACAGCTCGACCATGGTAGAGGCTG CACCACCGATTCCAAAGAGCCGCGGGAGGAAGCGAGCTGCTCAGCAGACCTCCGATATGACTGGCCCCTC TAGTAAGCAGGCGAAAGTCACCTACAGCTGTATTTACTGCAACAAGCAGTTATTTTCCAGTCTCGCGGTT CTGCAGATTCACTTGAAAACTATGCATTTAGATAAGCCTGAGCAGGCTCATATCTGTCAGTATTGCTTGG AGGTCTTACCCTCACTCTATAACCTAAATGAACATCTTAAACAAGTGCACGAAGCTCAGGACCCCGGCCT GATTGTTTCGGCCATGCCTGCCATTGTTTACCAGTGCAACTTCTGTTCCGAAGTTGTCAATGACCTCAAC ACCCTTCAGGAGCACATCCGATGTTCTCATGGGTTTGCCAATCCCGCGGCCAAGGACAGCAATGCGTTCT TTTGTCCCCATTGTTACATGGGGTTTCTCACTGACTCTTCACTTGAAGAGCATATAAGACAGGTCCATTG TGACCTCAGTGGCTCCCGGTTTGGGTCTCCTGTGCTTGGGACTCCAAAAGAACCGGTGGTTGAAGTCTAC TCCTGTTCCTATTGTACAAATTCGCCAATATTCAACAGTGTTCTTAAACTGAATAAGCATATTAAAGAGA ATCATAAAAACATTCCCTTGGCCCTGAATTATATTCACAATGGGAAGAAATCCCGGGCCTTGAGCCCTTT GTCCCCTGTGGCTATAGAACAAACAACTCTTAAGATGATGCAGACTGTGGGAGGCGGGCCTGCCCGCGCC TCTGGAGAGTATATCTGTAATCAGTGTGGTGCTAAGTACACGTCCCTAGACAGCTTTCAGACTCACCTCA AAACCCATTTGGACACCGTGCTGCCAAAACTGACCTGCCCTCAGTGTAACAAAGAATTCCCCAACCAAGA GTCCTTGCTAAAGCACGTGACCATCCACTTTATGATCACCTCAACCTACTACATCTGTGAGAGCTGTGAC AAGCAGTTCACCTCCGTGGATGACCTCCAGAAGCACCTGCTGGACATGCACACCTTCGTCTTCTTCCGCT GCACACTCTGCCAGGAAGTGTTCGACTCCAAGGTCTCTATCCAGCTGCACTTGGCCGTGAAGCACAGTAA CGAGAAGAAGGTGTACCGCTGCACGTCCTGCAACTGGGACTTCCGCAACGAGACCGACCTGCAGCTGCAC GTGAAGCACAACCACTTGGAGAACCAAGGCAAAGTGCACAAGTGCATCTTCTGCGGCGAGTCGTTTGGCA CCGAGGTGGAGCTGCAGTGCCACATCACCACCCACAGCAAGAAGTACAACTGCAGGTTCTGCAGCAAAGC CTTCCACGCCGTCATCCTGCTGGAGAAGCACCTGCGGGAGAAACACTGTGTGTTTGAAACCAAGACCCCC AACTGTGGCACCAACGGGGCCTCGGAGCAAGTGCAGAAGGAGGAGGCTGAGCTGCAGACCTTGCTCACCA ACAGCCAGGAGTCCCATAACAGCCATGATGGGAGTGAGGAGGACGTGGACAGCTCTGAACCCATGTATGG CTGTGACATCTGCGGGGCGGCCTACACCATGGAGACGTTGCTGCAGAACCACCAGCTCCGAGACCACAAC ATCAGGCCCGGAGAAAGCGCCATCGTGAAGAAGAAGGCGGAGCTCATTAAAGGGAATTACAAGTGTAACG TTTGCTCGAGAACCTTCTTCTCCGAAAACGGGCTCCGTGAACACATGCAGACCCACTTAGGCCCGGTCAA ACACTACATGTGCCCTATCTGTGGAGAGCGCTTCCCTTCCCTGTTAACTCTCACAGAGCACAAAGTCACA CACAGCAAGAGCCTGGATACCGGAAACTGTCGTATTTGTAAGATGCCCCTGCAGAGCGAAGAGGAGTTTT TGGAGCACTGCCAGATGCACCCTGACCTGAGGAATTCCCTGACTGGGTTTCGTTGTGTGGTGTGTATGCA GACTGTGACCTCCACCTTGGAACTCAAAATCCACGGGACCTTCCACATGCAGAAGACGGGGAATGGGTCC TCCGTGCAGACCACCGGGCGTGGCCAGCACGTTCAGAAACTGTATAAGTGCGCATCTTGCCTCAAAGAGT TCCGTTCCAAGCAAGATCTGGTGAAACTCGACATCAACGGCCTGCCATATGGTCTGTGTGCCGGCTGTGT GAATCTCAGTAAGAGCAGTAGCCCGGGCCTCAGTCTCCCTCCTGGTGCCAGCAGGCCAGGCTTGGGCCAG AATGAGAGTCTGAGCGCCATGGAAGGAAAAGGCAAGGCGGGGGGACTGAAGACGCGCTGTTCAAGCTGCA ATGTCAAGTTTGAGTCTGAAAGCGAACTGCAGAACCACATCCAGACGGTGCACCGGGAGCTTGTGCCAGA CGCCAACAGCACACAGTTGAAAACGCCCCAAGTGTCACCCATGCCCAGAATCAGTCCCTCCCAGTCCGAT GAGAAGAAGACCTACCAGTGTATCAAGTGTCAGATGGTTTTCTACAACGAGTGGGACATCCAGGTTCATG TGGCAAATCACATGATTGATGAAGGGCTGAACCATGAGTGCAAACTCTGCAGCCAGACCTTTGACTCCCC CGCCAAACTTCAGTGTCACCTGATAGAGCACAGCTTCGAAGGGATGGGTGGTACCTTCAAGTGCCCCGTC TGTTTCACAGTGTTTGTTCAAGCTAACAAGTTGCAACAGCATATATTCTCTGCCCATGGACAAGAAGACA AGATCTATGACTGCACACAATGCCCACAGAAGTTTTTCTTCCAAACAGAGCTACAGAATCATACGATGAC TCAGCACAGCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211913 representing NM_145492
Red=Cloning site Green=Tags(s) MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQC QLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSH SDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRG FLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAA LQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVG YTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAV LQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLN TLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVY SCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARA SGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCD KQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLH VKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTP NCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHN IRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVT HSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGS SVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQ NESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSD EKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPV CFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145492 |
| ORF Size | 3933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145492.4, NP_663467.1 |
| RefSeq Size | 6152 bp |
| RefSeq ORF | 3936 bp |
| Locus ID | 225207 |
| UniProt ID | Q6KAS7 |
| MW | 148.1 kDa |
| Gene Summary | Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lack of zinc finger protein 521 upregulates dopamine β-hydroxylase expression in the mouse brain, leading to abnormal behavior
,Ohkubo, N;Aoto, M;Kon, K;Mitsuda, N;,
Life Sci.
,PubMed ID 31200001
[ZFP521]
|
|
Siah2 Mediates Early Events in Commitment to an Adipogenic Pathway
,Kilroy, G;Burk, DH;Floyd, ZE;,
J. Biol. Chem.
,PubMed ID 27864366
[Zfp521]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204047 | Zfp521 (untagged) - Mouse zinc finger protein 521 (Zfp521), (10ug) |
CNY 9,136.00 |
|
| MR211913L3 | Lenti ORF clone of Zfp521 (Myc-DDK-tagged) - Mouse zinc finger protein 521 (Zfp521) |
CNY 13,400.00 |
|
| MR211913L4 | Lenti ORF clone of Zfp521 (mGFP-tagged) - Mouse zinc finger protein 521 (Zfp521) |
CNY 13,400.00 |
