Ncapd2 (BC029133) Mouse Tagged ORF Clone
CAT＃: MR211971
- TrueORF®
Ncapd2 (Myc-DDK-tagged) - Mouse non-SMC condensin I complex, subunit D2 (cDNA clone MGC:37090 IMAGE:4951596)
ORF Plasmid: tGFP
"BC029133" in other vectors (3)
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CNY 13970.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC39046, CNAP1, mKIAA0159, CAP-D2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211971 representing BC029133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCCCACAACTTTGAATTCCACTTGCCCTTATCTCCAGAGGAGCTGCTGAAAAGTGGAGGTGTGA ATCAGTACGTTGTCCGGGAGGTGCTGCCCGTCAAGCACCTCTCATCCCAGCTTAGAGCATTTCAGTCTGC CTTCCGAGCTCAGGGGCCCCTCGCCATACTGGAGCACTTCGATACAGTCTACAGCATTTTACATCACTTT CGAAGTATAGAACCTGGCCTCAAGGAAGACACTCTGGAATTCCTGAAGAAAGTGGTATCCCGCCACTCCC AGGAACTGTCGTCCATCCTGGATGATGCAGCTCTGAGTGGATCAGATAGAAGCGCCCACCTGAATGCCCT CAAAATGAACTGCTATGCCCTGATACGCCTTTTGGAGTCCTTTGAGAATATGACCAGCCAGACAAGCCTC ATTGACCTGGACATCGGTGGGAAGGGTAAGAGAGCCCGGGCCAAAGCAACCCTTGGCTTTGACTGGGAGG AAGAGAGGCAACCAGTTCTTCAGCTTTTAACACAGCTGCTCCAGCTGGACATCCGTCACCTATGGAACCA CTCAGCAATTGAAGAAGAATTTGTCAGTTTGGTTACTGGCTGTTGCTACCGCCTTTTGGAGAATCCCACC ATTAGTCACCAGAAGAACCGCTCCACCAAGGAGGCCATAGCACACCTGCTTGGGGTGGCCTTGGTCCGCT ACAACCATATGCTCAGTGCCACTGTGAAGATCATCCAGATGTTGCAGCACTTTGAGCACCTGCCCCCTGT GTTGGTGACAGCCGTGAGTCTGTGGGCAACAGACTACGGGATGAAGAGCATAGTGGGAGAGATTGTAAGA GAGATTGGACAGAAGTGTCCACAGGAGCTGAGTCGAGACACAGCAGGGGCAAAGGGCTTTGCGGCTTTCC TCACTGAGCTAGCAGAGCGAATCCCAGCTGTCCTGATGGCCAACATGTGCATTCTGCTAGACCATCTGGA TGGAGAGAATTACATGATGCGCAATGCTGTGTTAGCAGCTATAGCCGAGATGGTACTGCAGGTTCTAAAT GGTGACCAGCTCGAAGAATCAGCTCGAGAAACCAGAGATCAGTTCTTGGACATCTTGCAAGCTCATGGCC ATGATGTGAACTCTTTTGTTCGGAGCCGTGTTTTGCAGCTCTTTGCCCGAATTGTCCAACAGAAGGCACT CCCTCTGACCCGTTTCCAGGCAGTGGTGGCCTTGGCAGTGGGACGGCTGGCAGACAAGTCAGTGCTAGTG TGTAAAAATGCCATTCAGCTGCTGGCCAGCTTTCTAGCCAATAATCCCTTTTCCTGCAAGCTTAGTGATA TTGACCTTGCTGGACCACTGCAGAAGGAGATTCAGAAATTACAAGAAATGCGAGCCCAGAGGCGAAGTGC AGCAGCGACTGCAGCACTAGACCCGGAGGAGGAGTGGGATGCCATGTTGCCAGAATTGAAGTCGACCCTG CAGCAGCTCCTGAAACTTCCCCAGGAAGAGGGAGATCATCAAATTGCTGATGCAGAGACTGCAGAGGAGG TGAAAGGACGCATCCGCCAACTGCTTGCCAAAGCAAGTTACAAACAGGCTATTGTTCTCACTCGGGAAGC TACCAGTCACTTCCAGGAGTCTGAACCCTTCAGTCACACAGAGCCAGAGGAGAACAGCTTCCTGAATCTC TTGGGACTCATCTTCAAAGGCCCCGAAGCTTCCACACAGGACTCTCATGGGGACACAGACCCAGGGCTGA CAGGCAGTAAAGACTCTCCCAGTGTGCCCGAGCCAGAGGGATCCCAGAGCAACGATGAGCTGGTGAAGCA GGAGATGCTGGTGCAGTATCTGCAGGATGCCTACGGCTTCTCCCAGAAGATTACAGAAGCCATTGGCATC ATCAGCAAGATGATGTATGAGAACACAACTACAGTGGTGCAGGAGGTGATTGAGTTCTTTGTGATGGTAT TCCAATTTGGGGTACCTCAGGCCCTGTTTGGGGTACGACGCATGCTGCCTCTCATCTGGTCTAAGGAGCC TGGTGTCCGGGAAGCCGTGCTTAATGCGTACCGCCAACTCTACCTCAACCCGAAAGGGGACTCGGCCAGA GCCAAGGCGCAGACTTTGATTCACAACCTCTCGCTGTTGTTAGTGGATGCCTCCGTCGGGACCATTCAGT GTCTTGAGGAAATTCTTTGTGAGTTTGTGCAGAAGGATGAGGTAAAGCCAGCGGTGATCCAGCTGCTATG GGAACGGGCAACGGAGAAGGTGCCAAGCTCTCCTCTGGAGCGCTGCTCCTCTGTTATGCTTCTTGGCATG ATGGCACGAGGAAAACCAGAAATTGTAGGGAGCAACTTAGACGCCCTGGTGAGGGTGGGGCTGGATGAGA AGTCTCCTCAGGACTACAGGCTGGCCCAGCAGGTGTGCCTTGCCATTGCTAACATCTCGGACAGGAGGAA GCCTTCCCTGGGTGAGCGTCACCCTCCCTTCCGGCTGCCTCAGGAGCACAGGTTGTTTGAGCGACTTCAG GATATGGTCACAAAGGGCTTTGCACACCCAGATCCCCTCTGGATCCCATTCAAAGAAGTAGCAGTGACCC TGACCTACCAGTTGGCAGAGAGCCCTGATGTGCTCTGTGCCCAGATGTTGCAAGGCTGTGCCAAGCAAGT CTTGGAGAAACTTGAGAAGAACGCCACTGAGGCAGACCCCAAGGAAACAGCCCCTAGGCTCCCCACATTC CTGCTGATGAACCTGCTGTCCCTGGCTGGGGATGTGGCTCTTCAGCAGCTGGTGCATTTGGAACAAGCAG TGAGTGGAGAGCTGGGTCGGCGGCGAGTTCTGCGAGAGGAGCAGGAGCACAGGGCCAAGGAGCCCAAGGA GAAGACTGCAAGCTCTGAAACTACAATGGAGGAAGAACTGGGGCTGGTGGGGGGGGCCACTGCTGACGAC ACAGAGGCGGAGCTCATCCGAAGCATCTGTGAGAAGGAGCTGTTGGACGGCAACCAGGTACTAGCTGCCT TTGTTCCACTGCTACTTAAAGTCTGTAACAACCCTGGCTTATACAGCAACCCAGAGCTCTGTGCGGCTGC GTCGCTGGCCCTTGGCAAGTTCTGCATGATCAGTGCCCCTTTCTGCGACTCCCAGCTTCGTCTTCTATTC ACCATGCTGGAAAAGTCTTCACTCCCCACTGTGCGATCTAACCTCATGGTTGCCACAGGGGATCTGGCTA TCCGCTTCCCTAACCTGGTGGATCCCTGGACTCCTCATCTGTATGCCCGCCTCCGAGACCCAGCTCAACA AGTGCGGAAGACAGCGGGGCTGGTGATGACGCACCTGATCCTGAAGGACATGGTGAAGGTGAAGGGGCAG GTGAGCGAGATGGCCGTGCTGCTCATCGACCCTGTGCCTCAGATCGCTGCTCTGGCCAAGAATTTCTTCA ATGAGCTCTCCCACAAGGGCAATGCTATCTATAATCTACTTCCCGATATCATCAGTCGCCTGTCAGACCC AGAGGGCGGAGTGGAAGAGGAGCCCTTCCACACCATCATGAAGCAGCTCCTCTCCTACATCACCAAGGAC AAACAGACTGAGAGCTTGGTAGAAAAGCTGTGTCAGCGGTTCCGCACAGCCCGAACTGAGCGTCAGTACC GAGACCTAGCCTACTGTATGTCCCAGCTGCCCCTCACAGAGCGAGGTCTCCAGAAGATGCTAGACAACTT TGAGTGCTTTGGCGATAAGCTCTTAGATGAGTCTGTCTTCAGTGCTTTCTTGTCAATTGTGGGCAAACTG CGTCGCGGGGCCAAGCCTGAGGGCAAGGCTATAATAGATGAATTTGAGCAGAAGCTTCGTGCCTGTCACA CCAGAGGCATGGATGGAATAGAGGAGTTTGAAACTGGCCAGGGAGGCAGCCAGCGAGCCCTGTCTGCCAA GAAACCCTCCGCTGTCTCAAGGCTACAGCCTCTGACTTCTGTAGACTCAGACAATGACTTTGTCACACCT AAGCCACGACGTACCAAACCTGGTCGTCCACAAACTCAGCAGCGCAAGAAGTCCCAGAGGAAAGCCAAAG TTGTCTTCTTGAGTGACGAGTCCAGTGAGGACGAACTTTCAGCAGAGATGACAGAAGAAGAGACACCCAA GAGAACCACCCCCACCCTTGTAGAAGTGCTTGTTGTCTGTGGTTTTAACATGGAAATGGCCTTTGCATTG AGGTCTTTCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211971 representing BC029133
Red=Cloning site Green=Tags(s) MSPHNFEFHLPLSPEELLKSGGVNQYVVREVLPVKHLSSQLRAFQSAFRAQGPLAILEHFDTVYSILHHF RSIEPGLKEDTLEFLKKVVSRHSQELSSILDDAALSGSDRSAHLNALKMNCYALIRLLESFENMTSQTSL IDLDIGGKGKRARAKATLGFDWEEERQPVLQLLTQLLQLDIRHLWNHSAIEEEFVSLVTGCCYRLLENPT ISHQKNRSTKEAIAHLLGVALVRYNHMLSATVKIIQMLQHFEHLPPVLVTAVSLWATDYGMKSIVGEIVR EIGQKCPQELSRDTAGAKGFAAFLTELAERIPAVLMANMCILLDHLDGENYMMRNAVLAAIAEMVLQVLN GDQLEESARETRDQFLDILQAHGHDVNSFVRSRVLQLFARIVQQKALPLTRFQAVVALAVGRLADKSVLV CKNAIQLLASFLANNPFSCKLSDIDLAGPLQKEIQKLQEMRAQRRSAAATAALDPEEEWDAMLPELKSTL QQLLKLPQEEGDHQIADAETAEEVKGRIRQLLAKASYKQAIVLTREATSHFQESEPFSHTEPEENSFLNL LGLIFKGPEASTQDSHGDTDPGLTGSKDSPSVPEPEGSQSNDELVKQEMLVQYLQDAYGFSQKITEAIGI ISKMMYENTTTVVQEVIEFFVMVFQFGVPQALFGVRRMLPLIWSKEPGVREAVLNAYRQLYLNPKGDSAR AKAQTLIHNLSLLLVDASVGTIQCLEEILCEFVQKDEVKPAVIQLLWERATEKVPSSPLERCSSVMLLGM MARGKPEIVGSNLDALVRVGLDEKSPQDYRLAQQVCLAIANISDRRKPSLGERHPPFRLPQEHRLFERLQ DMVTKGFAHPDPLWIPFKEVAVTLTYQLAESPDVLCAQMLQGCAKQVLEKLEKNATEADPKETAPRLPTF LLMNLLSLAGDVALQQLVHLEQAVSGELGRRRVLREEQEHRAKEPKEKTASSETTMEEELGLVGGATADD TEAELIRSICEKELLDGNQVLAAFVPLLLKVCNNPGLYSNPELCAAASLALGKFCMISAPFCDSQLRLLF TMLEKSSLPTVRSNLMVATGDLAIRFPNLVDPWTPHLYARLRDPAQQVRKTAGLVMTHLILKDMVKVKGQ VSEMAVLLIDPVPQIAALAKNFFNELSHKGNAIYNLLPDIISRLSDPEGGVEEEPFHTIMKQLLSYITKD KQTESLVEKLCQRFRTARTERQYRDLAYCMSQLPLTERGLQKMLDNFECFGDKLLDESVFSAFLSIVGKL RRGAKPEGKAIIDEFEQKLRACHTRGMDGIEEFETGQGGSQRALSAKKPSAVSRLQPLTSVDSDNDFVTP KPRRTKPGRPQTQQRKKSQRKAKVVFLSDESSEDELSAEMTEEETPKRTTPTLVEVLVVCGFNMEMAFAL RSFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC029133 |
| ORF Size | 4212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029133.1 |
| RefSeq Size | 4376 bp |
| RefSeq ORF | 4214 bp |
| Locus ID | 68298 |
| MW | 160.4 kDa |
| Gene Summary | Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211971 | Ncapd2 (tGFP-tagged) - Mouse non-SMC condensin I complex, subunit D2 (cDNA clone MGC:37090 IMAGE:4951596) |
CNY 15390.00 |
|
| MR211971L3 | Lenti ORF clone of Ncapd2 (Myc-DDK-tagged) - Mouse non-SMC condensin I complex, subunit D2 (cDNA clone MGC:37090 IMAGE:4951596) |
CNY 15870.00 |
|
| MR211971L4 | Lenti ORF clone of Ncapd2 (mGFP-tagged) - Mouse non-SMC condensin I complex, subunit D2 (cDNA clone MGC:37090 IMAGE:4951596) |
CNY 15870.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
