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Col5a2 (NM_007737) Mouse Tagged ORF Clone
CAT＃: MR212011
- TrueORF®
Col5a2 (Myc-DDK-tagged) - Mouse collagen, type V, alpha 2 (Col5a2)
ORF Plasmid: tGFP
"NM_007737" in other vectors (4)
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CNY 10,240.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110014L14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212011 representing NM_007737
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCAAACTGGGTGGGAGCAAGACCTCTCCTCATTCTTTCTGTTTTATTAGGGTACTGTGTCTCAA TAAAAGCCCAGGAACAAGAGAATGATGAATATGATGAAGAAATAGCCTGTACTCAACATGGACAGATGTA TTTAAATAGAGACATTTGGAAACCTTCTCCATGTCAGATCTGTGTCTGTGACAATGGTGCCATTCTTTGT GACAAGATAGAGTGCCCAGAGGTGCTCAACTGTGCCAATCCTATAACACCCACTGGGGAGTGCTGTCCGG TGTGCCCACAAACTGGAGGTGGAGATACTAGTTTTGGTAGAGGAAGAAAGGGACAAAAAGGAGAACCAGG ATTAGTGCCTGTTGTAACAGGTATACGCGGTCGTCCAGGACCTGCAGGCCCCCCAGGATCACAAGGACCA AGAGGGGACCGAGGCCCTAAAGGAAGACCTGGTCCTCGTGGTCCTCAGGGAATTGATGGAGAGCCAGGTG TGCCTGGTCAGCCAGGTGCTCCAGGGCCTCCTGGGCACCCCTCTCACCCAGGACCTGATGGCATGAGCAG GCCTTTTTCAGCTCAGATGGCTGGGCTGGATGAAAAATCTGGACTTGGAAGTCAAGTAGGACTCATGCCT GGCTCTGTGGGTCCTGTTGGCCCAAGAGGACCTCAGGGTTTACAAGGACAGCAAGGTGGTGTGGGACCCG CAGGACCTCCTGGTGAGCCTGGCGAGCCTGGACCAATGGGTCCAATTGGTTCTCGTGGACCAGAGGGCCC ACCTGGGAAGCCCGGAGAAGATGGTGAACCTGGAAGAAATGGGAATACAGGTGAAGTGGGATTCTCAGGA TCACCAGGTGCTCGAGGCTTCCCTGGTGCTCCTGGTCTCCCTGGCTTGAAAGGTCACAGAGGACACAAAG GTCTTGAAGGCCCCAAAGGTGAAATTGGAGCACCTGGTGCTAAGGGTGAAGCTGGCCCCACTGGTCCAAT GGGTGCTATGGGCCCTCTGGGACCAAGAGGAATGCCAGGAGAGAGGGGAAGACTTGGGCCACAGGGTGCT CCTGGAAAACGGGGTGCCCACGGTATGCCTGGAAAACCTGGACCAATGGGTCCTCTTGGGATACCTGGCT CTTCCGGTTTTCCAGGAAATCCTGGAATGAAGGGAGAAGCAGGTCCTACTGGAGCCCGAGGCCCTGAAGG TCCTCAGGGGCAGAGAGGTGAAACTGGGCCACCTGGTCCAGCTGGCTCTCAGGGTCTTCCTGGTGCAGTG GGGACTGATGGTACACCTGGCGCCAAAGGCCCCACAGGCTCTGCAGGAACCTCTGGCCCTCCTGGCTTGG CAGGGCCCCCTGGGTCTCCTGGCCCTCAAGGCAGTACTGGACCTCAGGGAATCCGAGGACAATCGGGTGA TCCAGGAGTTCCAGGTTTCAAAGGAGAAGCTGGTCCAAAGGGAGAACCAGGGCCACATGGTATTCAGGGT CCCATTGGTCCACCTGGTGAAGAAGGCAAAAGAGGTCCTCGGGGTGATCCAGGCACAGTTGGTCCTCCAG GACCAATGGGAGAAAGGGGCGCTCCTGGCAATCGTGGTTTTCCAGGGTCTGATGGTTTACCTGGACCAAA GGGTGCTCAGGGAGAGCGTGGTCCTGTAGGTTCTTCGGGGCCTAAAGGAGGCCAGGGAGATCCAGGACGT CCAGGAGAACCTGGGCTTCCAGGTGCTCGGGGTCTGACAGGAAATCCTGGTGTTCAAGGTCCTGAAGGAA AACTTGGGCCTTTGGGTGCTCCTGGGGAAGATGGTCGGCCAGGTCCTCCAGGCTCCATTGGGATCAGAGG ACAGCCTGGAAGCATGGGCCTTCCAGGCCCCAAAGGTAGCAGTGGTGACCTTGGAAAACCAGGAGAAGCA GGAAATGCTGGTGTGCCTGGGCAAAGGGGGGCTCCTGGCAAAGATGGAGAAGTTGGTCCTTCAGGCCCTG TGGGACCTCCGGGCCTAGCTGGGGAAAGAGGAGAGCAGGGACCTCCAGGCCCCACTGGCTTTCAGGGACT TCCTGGACCTCCAGGGCCACCTGGAGAAGGTGGAAAGGCTGGTGATCAAGGTGTTCCTGGAGAGCCTGGA GCAGTTGGCCCATTAGGACCTCGGGGAGAAAGAGGGAATCCTGGAGAAAGAGGAGAGCCAGGGATAACTG GACTGCCTGGGGAGAAAGGCATGGCTGGAGGACATGGCCCTGATGGTCCAAAGGGCAACCCAGGTCCAAC TGGGACCATTGGAGACACAGGGCCACCAGGTCTTCAGGGCATGCCAGGAGAAAGAGGAATTGCAGGAACA CCTGGCCCTAAGGGTGACCGAGGTGGCATAGGAGAGAAAGGTGCTGAAGGAACAGCAGGAAATGACGGGG CAAGGGGTCTTCCAGGTCCCTTGGGACCTCCAGGTCCTGCAGGCCCTACTGGAGAAAAGGGTGAACCTGG TCCTAGAGGCTTAGTTGGCCCTCCTGGCTCCCGTGGCAATCCTGGTTCTCGTGGTGAAAATGGTCCAACT GGAGCTGTTGGTTTTGCTGGACCCCAGGGTCCCGATGGACAGCCTGGTGTCAAGGGTGAGCCTGGAGAGC CAGGACAGAAGGGAGATGCTGGGTCTCCTGGACCCCAAGGGCTAGCAGGATCACCTGGCCCCCATGGTCC ACATGGTGTTCCTGGACTGAAAGGCGGTAGAGGAACCCAGGGTCCACCTGGTGCTACAGGGTTTCCTGGT TCTGCTGGCAGAGTTGGGCCTCCAGGCCCTGCTGGAGCTCCAGGACCTGCAGGGCCCGCAGGAGAACCTG GGAAGGAAGGACCTCCTGGTCTTCGTGGTGACCCTGGCTCTCATGGGCGAGTGGGAGATAGAGGACCAGC TGGCCCACCTGGTAGCCCAGGAGATAAAGGGGACCCAGGAGAAGATGGACAACCTGGTCCAGATGGTCCT CCTGGTCCCGCTGGTACCACTGGGCAAAGAGGAATTGTTGGAATGCCCGGACAACGTGGGGAACGAGGAA TGCCTGGCCTTCCTGGCCCAGCGGGTACACCAGGAAAAGTAGGACCAACTGGTGCGACAGGAGATAAAGG TCCCCCTGGACCTGTAGGACCTCCAGGTTCCAATGGTCCTGTAGGAGAACCTGGGCCAGAAGGTCCGGCT GGTAATGATGGTACCCCAGGACGGGATGGTGCTGTTGGAGAACGGGGTGACCGTGGAGACCCCGGGCCTG CTGGTCTACCAGGCTCTCAGGGTGCCCCTGGAACTCCTGGCCCTGTTGGTGCCCCTGGAGATGCAGGACA AAGAGGAGAGCCAGGTTCTCGAGGCCCTGTAGGACCACCTGGTCGAGCTGGAAAGCGTGGTTTACCTGGA CCACAAGGACCTCGAGGTGACAAGGGTGACAATGGAGACAGAGGTGACCGAGGTCAGAAAGGCCACAGAG GGTTCACTGGCCTGCAGGGTCTTCCTGGACCTCCTGGTCCAAATGGTGAACAAGGAAGTGCTGGCATTCC TGGTCCTTTCGGTCCAAGAGGTCCTCCAGGACCAGTAGGTCCTTCAGGAAAGGAAGGAAACCCTGGGCCA CTTGGACCAATTGGACCTCCTGGTGTGCGGGGCAGTGTAGGAGAAGCAGGACCAGAGGGTCCTCCTGGTG AACCTGGGCCTCCTGGCCCTCCAGGTCCCCCTGGCCATCTTACTGCTGCTCTTGGTGATATCATGGGACA CTATGATGAGAACATGCCAGATCCACTTCCAGAGTTTACGGAAGACCAGGCAGCTCCAGATGACACAAAC AAAACAGACCCTGGGATCCATGTTACCCTGAAGTCTCTCAGTAGTCAGATTGAAACCATGCGTAGCCCTG ATGGTTCCAAGAAACACCCAGCCCGCACTTGTGATGATTTAAAGCTTTGCCATCCCACAAAGCAGAGCGG TGAATATTGGATTGATCCTAACCAGGGATCAGCTGAAGATGCAATCAAAGTTTACTGCAATATGGAAACA GGAGAGACATGTATTTCAGCAAACCCAGCCAGTGTTCCACGTAAAACCTGGTGGGCCAGCAAATCCCCTG ACAATAAGCCTGTGTGGTACGGTCTTGACATGAATAGAGGATCTCAGTTCACATATGGAGATTACCAGTC TCCTAACACAGCTATCACTCAGATGACCTTTTTGCGTCTTTTATCAAAAGAAGCCTCGCAGAACCTTACT TACATCTGTAGGAACACTGTTGGATATATGGATGATCAAGCTAAAAACCTCAAGAAAGCTGTGGTCCTTA AAGGGTCAAACGACTTAGAAATAAAAGGAGAAGGAAACATCAGATTCAGATACACAGTTCTTCAAGACAC CTGCTCTAAGCGAAATGGAAATGTAGGCAAAACTATCTTTGAATACAGAACACAGAATGTGGCCCGCTTG CCCATCATAGATGTTGGTCCTGTGGATATTGGCAATGCAGACCAGGAATTTGGCCTTGATATTGGGCCAG TTTGTTTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212011 representing NM_007737
Red=Cloning site Green=Tags(s) MMANWVGARPLLILSVLLGYCVSIKAQEQENDEYDEEIACTQHGQMYLNRDIWKPSPCQICVCDNGAILC DKIECPEVLNCANPITPTGECCPVCPQTGGGDTSFGRGRKGQKGEPGLVPVVTGIRGRPGPAGPPGSQGP RGDRGPKGRPGPRGPQGIDGEPGVPGQPGAPGPPGHPSHPGPDGMSRPFSAQMAGLDEKSGLGSQVGLMP GSVGPVGPRGPQGLQGQQGGVGPAGPPGEPGEPGPMGPIGSRGPEGPPGKPGEDGEPGRNGNTGEVGFSG SPGARGFPGAPGLPGLKGHRGHKGLEGPKGEIGAPGAKGEAGPTGPMGAMGPLGPRGMPGERGRLGPQGA PGKRGAHGMPGKPGPMGPLGIPGSSGFPGNPGMKGEAGPTGARGPEGPQGQRGETGPPGPAGSQGLPGAV GTDGTPGAKGPTGSAGTSGPPGLAGPPGSPGPQGSTGPQGIRGQSGDPGVPGFKGEAGPKGEPGPHGIQG PIGPPGEEGKRGPRGDPGTVGPPGPMGERGAPGNRGFPGSDGLPGPKGAQGERGPVGSSGPKGGQGDPGR PGEPGLPGARGLTGNPGVQGPEGKLGPLGAPGEDGRPGPPGSIGIRGQPGSMGLPGPKGSSGDLGKPGEA GNAGVPGQRGAPGKDGEVGPSGPVGPPGLAGERGEQGPPGPTGFQGLPGPPGPPGEGGKAGDQGVPGEPG AVGPLGPRGERGNPGERGEPGITGLPGEKGMAGGHGPDGPKGNPGPTGTIGDTGPPGLQGMPGERGIAGT PGPKGDRGGIGEKGAEGTAGNDGARGLPGPLGPPGPAGPTGEKGEPGPRGLVGPPGSRGNPGSRGENGPT GAVGFAGPQGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGSPGPHGPHGVPGLKGGRGTQGPPGATGFPG SAGRVGPPGPAGAPGPAGPAGEPGKEGPPGLRGDPGSHGRVGDRGPAGPPGSPGDKGDPGEDGQPGPDGP PGPAGTTGQRGIVGMPGQRGERGMPGLPGPAGTPGKVGPTGATGDKGPPGPVGPPGSNGPVGEPGPEGPA GNDGTPGRDGAVGERGDRGDPGPAGLPGSQGAPGTPGPVGAPGDAGQRGEPGSRGPVGPPGRAGKRGLPG PQGPRGDKGDNGDRGDRGQKGHRGFTGLQGLPGPPGPNGEQGSAGIPGPFGPRGPPGPVGPSGKEGNPGP LGPIGPPGVRGSVGEAGPEGPPGEPGPPGPPGPPGHLTAALGDIMGHYDENMPDPLPEFTEDQAAPDDTN KTDPGIHVTLKSLSSQIETMRSPDGSKKHPARTCDDLKLCHPTKQSGEYWIDPNQGSAEDAIKVYCNMET GETCISANPASVPRKTWWASKSPDNKPVWYGLDMNRGSQFTYGDYQSPNTAITQMTFLRLLSKEASQNLT YICRNTVGYMDDQAKNLKKAVVLKGSNDLEIKGEGNIRFRYTVLQDTCSKRNGNVGKTIFEYRTQNVARL PIIDVGPVDIGNADQEFGLDIGPVCFM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007737 |
| ORF Size | 4491 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007737.2, NP_031763.2 |
| RefSeq Size | 6615 bp |
| RefSeq ORF | 4494 bp |
| Locus ID | 12832 |
| UniProt ID | Q3U962 |
| MW | 145 kDa |
| Gene Summary | This gene encodes the alpha-2 subunit of type V collagen, one of the low abundance fibrillar collagens that gets incorporated into growing fibrils with type I collagen. The encoded protein, in association with alpha-1 and/or alpha-3 subunits, forms homo- or heterotrimeric type V procollagen that undergoes proteolytic processing. Mice lacking the encoded protein die in utero. Transgenic mice that produce a structurally abnormal form of the encoded protein survive poorly and exhibit skin fragility, skeletal abnormalities and alterations in the collagen fiber organization. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204736 | Col5a2 (untagged) - Mouse collagen, type V, alpha 2 (Col5a2), (10ug) |
CNY 10,248.00 |
|
| MG212011 | Col5a2 (tGFP-tagged) - Mouse procollagen, type V, alpha 2 (Col5a2) |
CNY 14,440.00 |
|
| MR212011L3 | Lenti ORF clone of Col5a2 (Myc-DDK-tagged) - Mouse collagen, type V, alpha 2 (Col5a2) |
CNY 15,010.00 |
|
| MR212011L4 | Lenti ORF clone of Col5a2 (mGFP-tagged) - Mouse collagen, type V, alpha 2 (Col5a2) |
CNY 15,010.00 |
